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Human monocyte heat shock protein 72 responses to acute hypoxic exercise after 3 days of exercise heat acclimation.

Lee BJ, Mackenzie RW, Cox V, James RS, Thake CD - Biomed Res Int (2015)

Bottom Line: Basal mHSP72 remained elevated before HST2 for the STHA group (P < 0.05) and was unchanged from HST1 in CON (P > 0.05).Percent change in mHSP72 was lower after HST2 in STHA compared to CON (P = 0.02).This is indicative of improved tolerance and ability to cope with the hypoxic insult, potentially mediated in part by increased basal reserves of HSP72.

View Article: PubMed Central - PubMed

Affiliation: Exercise Science Applied Research Group, Coventry University, Priory Street, Coventry CV1 5FB, UK.

ABSTRACT
The aim of this study was to determine whether short-term heat acclimation (STHA) could confer increased cellular tolerance to acute hypoxic exercise in humans as determined via monocyte HSP72 (mHSP72) expression. Sixteen males were separated into two matched groups. The STHA group completed 3 days of exercise heat acclimation; 60 minutes cycling at 50% V̇O2peak in 40°C 20% relative humidity (RH). The control group (CON) completed 3 days of exercise training in 20°C, 40% RH. Each group completed a hypoxic stress test (HST) one week before and 48 hours following the final day of CON or STHA. Percentage changes in HSP72 concentrations were similar between STHA and CON following HST1 (P = 0.97). STHA induced an increase in basal HSP72 (P = 0.03) with no change observed in CON (P = 0.218). Basal mHSP72 remained elevated before HST2 for the STHA group (P < 0.05) and was unchanged from HST1 in CON (P > 0.05). Percent change in mHSP72 was lower after HST2 in STHA compared to CON (P = 0.02). The mHSP72 response to hypoxic exercise was attenuated following 3 days of heat acclimation. This is indicative of improved tolerance and ability to cope with the hypoxic insult, potentially mediated in part by increased basal reserves of HSP72.

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Regression analysis between preexercise monocyte expression of mHSP72 and the fold change (%) in mHSP72 following HST1 (a) and HST2 (b). Black circles denote CON and red diamonds denote STHA. Prior to HST1 the percentage change in mHSP72 after exercise had an inverse relationship with basal mHSP72. This feature was present in both CON (black circles) and STHA (red diamonds). After the intervention period the inverse relationship was still present in the CON (b), but no longer a feature of the STHA group, possibly as a result of the increase in basal mHSP72 observed prior to onset of HST2 (Figure 6(b)).
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fig7: Regression analysis between preexercise monocyte expression of mHSP72 and the fold change (%) in mHSP72 following HST1 (a) and HST2 (b). Black circles denote CON and red diamonds denote STHA. Prior to HST1 the percentage change in mHSP72 after exercise had an inverse relationship with basal mHSP72. This feature was present in both CON (black circles) and STHA (red diamonds). After the intervention period the inverse relationship was still present in the CON (b), but no longer a feature of the STHA group, possibly as a result of the increase in basal mHSP72 observed prior to onset of HST2 (Figure 6(b)).

Mentions: The initial HST produced an increase in mHSP72 in CON (34 ± 51%) and STHA (39 ± 37%). This response was not different between groups (P > 0.05). Resting mHSP72 was elevated from HST1 to HST2 in STHA (28 ± 26%, P < 0.05; d = 0.94) and unchanged for CON (3 ± 27%; d = −0.08). The mHSP72 response to HST2 was similar to HST1 in the CON group (48 ± 30%). STHA attenuated in post-HST2 mHSP72 expression compared to HST1 (98 ± 12%; P < 0.05) and was lower after exercise in STHA compared to CON (P < 0.05; d = −0.45) (Figure 6). Significant correlations were observed for the preexercise mHSP72 expression and the percentage change in expression following exercise for HST1 in both the CON and STHA groups (Figure 7(a)) and were also present during HST2 for the CON group (Figure 7(b)). This relationship was weakened for HST2 in the STHA group (Figure 7(b)).


Human monocyte heat shock protein 72 responses to acute hypoxic exercise after 3 days of exercise heat acclimation.

Lee BJ, Mackenzie RW, Cox V, James RS, Thake CD - Biomed Res Int (2015)

Regression analysis between preexercise monocyte expression of mHSP72 and the fold change (%) in mHSP72 following HST1 (a) and HST2 (b). Black circles denote CON and red diamonds denote STHA. Prior to HST1 the percentage change in mHSP72 after exercise had an inverse relationship with basal mHSP72. This feature was present in both CON (black circles) and STHA (red diamonds). After the intervention period the inverse relationship was still present in the CON (b), but no longer a feature of the STHA group, possibly as a result of the increase in basal mHSP72 observed prior to onset of HST2 (Figure 6(b)).
© Copyright Policy
Related In: Results  -  Collection

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Show All Figures
getmorefigures.php?uid=PMC4385626&req=5

fig7: Regression analysis between preexercise monocyte expression of mHSP72 and the fold change (%) in mHSP72 following HST1 (a) and HST2 (b). Black circles denote CON and red diamonds denote STHA. Prior to HST1 the percentage change in mHSP72 after exercise had an inverse relationship with basal mHSP72. This feature was present in both CON (black circles) and STHA (red diamonds). After the intervention period the inverse relationship was still present in the CON (b), but no longer a feature of the STHA group, possibly as a result of the increase in basal mHSP72 observed prior to onset of HST2 (Figure 6(b)).
Mentions: The initial HST produced an increase in mHSP72 in CON (34 ± 51%) and STHA (39 ± 37%). This response was not different between groups (P > 0.05). Resting mHSP72 was elevated from HST1 to HST2 in STHA (28 ± 26%, P < 0.05; d = 0.94) and unchanged for CON (3 ± 27%; d = −0.08). The mHSP72 response to HST2 was similar to HST1 in the CON group (48 ± 30%). STHA attenuated in post-HST2 mHSP72 expression compared to HST1 (98 ± 12%; P < 0.05) and was lower after exercise in STHA compared to CON (P < 0.05; d = −0.45) (Figure 6). Significant correlations were observed for the preexercise mHSP72 expression and the percentage change in expression following exercise for HST1 in both the CON and STHA groups (Figure 7(a)) and were also present during HST2 for the CON group (Figure 7(b)). This relationship was weakened for HST2 in the STHA group (Figure 7(b)).

Bottom Line: Basal mHSP72 remained elevated before HST2 for the STHA group (P < 0.05) and was unchanged from HST1 in CON (P > 0.05).Percent change in mHSP72 was lower after HST2 in STHA compared to CON (P = 0.02).This is indicative of improved tolerance and ability to cope with the hypoxic insult, potentially mediated in part by increased basal reserves of HSP72.

View Article: PubMed Central - PubMed

Affiliation: Exercise Science Applied Research Group, Coventry University, Priory Street, Coventry CV1 5FB, UK.

ABSTRACT
The aim of this study was to determine whether short-term heat acclimation (STHA) could confer increased cellular tolerance to acute hypoxic exercise in humans as determined via monocyte HSP72 (mHSP72) expression. Sixteen males were separated into two matched groups. The STHA group completed 3 days of exercise heat acclimation; 60 minutes cycling at 50% V̇O2peak in 40°C 20% relative humidity (RH). The control group (CON) completed 3 days of exercise training in 20°C, 40% RH. Each group completed a hypoxic stress test (HST) one week before and 48 hours following the final day of CON or STHA. Percentage changes in HSP72 concentrations were similar between STHA and CON following HST1 (P = 0.97). STHA induced an increase in basal HSP72 (P = 0.03) with no change observed in CON (P = 0.218). Basal mHSP72 remained elevated before HST2 for the STHA group (P < 0.05) and was unchanged from HST1 in CON (P > 0.05). Percent change in mHSP72 was lower after HST2 in STHA compared to CON (P = 0.02). The mHSP72 response to hypoxic exercise was attenuated following 3 days of heat acclimation. This is indicative of improved tolerance and ability to cope with the hypoxic insult, potentially mediated in part by increased basal reserves of HSP72.

Show MeSH
Related in: MedlinePlus