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Human monocyte heat shock protein 72 responses to acute hypoxic exercise after 3 days of exercise heat acclimation.

Lee BJ, Mackenzie RW, Cox V, James RS, Thake CD - Biomed Res Int (2015)

Bottom Line: Basal mHSP72 remained elevated before HST2 for the STHA group (P < 0.05) and was unchanged from HST1 in CON (P > 0.05).Percent change in mHSP72 was lower after HST2 in STHA compared to CON (P = 0.02).This is indicative of improved tolerance and ability to cope with the hypoxic insult, potentially mediated in part by increased basal reserves of HSP72.

View Article: PubMed Central - PubMed

Affiliation: Exercise Science Applied Research Group, Coventry University, Priory Street, Coventry CV1 5FB, UK.

ABSTRACT
The aim of this study was to determine whether short-term heat acclimation (STHA) could confer increased cellular tolerance to acute hypoxic exercise in humans as determined via monocyte HSP72 (mHSP72) expression. Sixteen males were separated into two matched groups. The STHA group completed 3 days of exercise heat acclimation; 60 minutes cycling at 50% V̇O2peak in 40°C 20% relative humidity (RH). The control group (CON) completed 3 days of exercise training in 20°C, 40% RH. Each group completed a hypoxic stress test (HST) one week before and 48 hours following the final day of CON or STHA. Percentage changes in HSP72 concentrations were similar between STHA and CON following HST1 (P = 0.97). STHA induced an increase in basal HSP72 (P = 0.03) with no change observed in CON (P = 0.218). Basal mHSP72 remained elevated before HST2 for the STHA group (P < 0.05) and was unchanged from HST1 in CON (P > 0.05). Percent change in mHSP72 was lower after HST2 in STHA compared to CON (P = 0.02). The mHSP72 response to hypoxic exercise was attenuated following 3 days of heat acclimation. This is indicative of improved tolerance and ability to cope with the hypoxic insult, potentially mediated in part by increased basal reserves of HSP72.

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The intervention period did not induce any changes in postexercise eHSP72 in CON (black lines (a)). Postexercise eHSP72 was elevated from rest after exercise on HA1 and HA3 in the STHA group (P < 0.001, red lines (b)). Lines represent individual data.
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fig3: The intervention period did not induce any changes in postexercise eHSP72 in CON (black lines (a)). Postexercise eHSP72 was elevated from rest after exercise on HA1 and HA3 in the STHA group (P < 0.001, red lines (b)). Lines represent individual data.

Mentions: eHSP72 remained unchanged from rest following exercise on each day of the 3-day protocol in the CON group (P > 0.05, Figure 3(a)). eHSP72 increased following exercise on day 1 and day 3 of the acclimation period (day 1: 1.06 ± 0.74 ng·mL−1; day 3: 1.04 ± 1.06 ng·mL−1; P < 0.001) in STHA (Figure 3(b)). Resting eHSP72 was lower (n = 6) on day 3 of STHA compared to day 1 (day 1: 1.43 ± 0.15 ng·mL−1; day 3: 1.12 ± 0.54 ng·mL−1), although this observation failed to reach statistical significance due to the high intersubject variability (P > 0.05; Figure 3(b)).


Human monocyte heat shock protein 72 responses to acute hypoxic exercise after 3 days of exercise heat acclimation.

Lee BJ, Mackenzie RW, Cox V, James RS, Thake CD - Biomed Res Int (2015)

The intervention period did not induce any changes in postexercise eHSP72 in CON (black lines (a)). Postexercise eHSP72 was elevated from rest after exercise on HA1 and HA3 in the STHA group (P < 0.001, red lines (b)). Lines represent individual data.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4385626&req=5

fig3: The intervention period did not induce any changes in postexercise eHSP72 in CON (black lines (a)). Postexercise eHSP72 was elevated from rest after exercise on HA1 and HA3 in the STHA group (P < 0.001, red lines (b)). Lines represent individual data.
Mentions: eHSP72 remained unchanged from rest following exercise on each day of the 3-day protocol in the CON group (P > 0.05, Figure 3(a)). eHSP72 increased following exercise on day 1 and day 3 of the acclimation period (day 1: 1.06 ± 0.74 ng·mL−1; day 3: 1.04 ± 1.06 ng·mL−1; P < 0.001) in STHA (Figure 3(b)). Resting eHSP72 was lower (n = 6) on day 3 of STHA compared to day 1 (day 1: 1.43 ± 0.15 ng·mL−1; day 3: 1.12 ± 0.54 ng·mL−1), although this observation failed to reach statistical significance due to the high intersubject variability (P > 0.05; Figure 3(b)).

Bottom Line: Basal mHSP72 remained elevated before HST2 for the STHA group (P < 0.05) and was unchanged from HST1 in CON (P > 0.05).Percent change in mHSP72 was lower after HST2 in STHA compared to CON (P = 0.02).This is indicative of improved tolerance and ability to cope with the hypoxic insult, potentially mediated in part by increased basal reserves of HSP72.

View Article: PubMed Central - PubMed

Affiliation: Exercise Science Applied Research Group, Coventry University, Priory Street, Coventry CV1 5FB, UK.

ABSTRACT
The aim of this study was to determine whether short-term heat acclimation (STHA) could confer increased cellular tolerance to acute hypoxic exercise in humans as determined via monocyte HSP72 (mHSP72) expression. Sixteen males were separated into two matched groups. The STHA group completed 3 days of exercise heat acclimation; 60 minutes cycling at 50% V̇O2peak in 40°C 20% relative humidity (RH). The control group (CON) completed 3 days of exercise training in 20°C, 40% RH. Each group completed a hypoxic stress test (HST) one week before and 48 hours following the final day of CON or STHA. Percentage changes in HSP72 concentrations were similar between STHA and CON following HST1 (P = 0.97). STHA induced an increase in basal HSP72 (P = 0.03) with no change observed in CON (P = 0.218). Basal mHSP72 remained elevated before HST2 for the STHA group (P < 0.05) and was unchanged from HST1 in CON (P > 0.05). Percent change in mHSP72 was lower after HST2 in STHA compared to CON (P = 0.02). The mHSP72 response to hypoxic exercise was attenuated following 3 days of heat acclimation. This is indicative of improved tolerance and ability to cope with the hypoxic insult, potentially mediated in part by increased basal reserves of HSP72.

Show MeSH
Related in: MedlinePlus