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Crocetin downregulates the proinflammatory cytokines in methylcholanthrene-induced rodent tumor model and inhibits COX-2 expression in cervical cancer cells.

Chen B, Hou ZH, Dong Z, Li CD - Biomed Res Int (2015)

Bottom Line: The inducible nitric oxide synthase (iNOS) activation in HeLa cells was analyzed using fluorescence microscopy for light microscopic examination.At the same time, the mRNA level of COX-2 in HeLa cells was also significantly increased.These results suggest that crocetin may act as a chemopreventive and an anti-inflammatory agent.

View Article: PubMed Central - PubMed

Affiliation: Department of Obstetrics and Gynaecology, Air Force General Hospital, PLA, Beijing 100142, China.

ABSTRACT
The effect of crocetin (C20H24O4) on methylcholanthrene- (MCA-) induced uterine cervical cancer in mice was studied in this paper. After the mice were treated orally with crocetin, maleic dialdehyde (MDA), polymorphonuclear cells (PMN), interleukin-1β (IL-1β), and tumor necrosis factor-α (TNF-α) were examined by ELISA or immunohistochemistry. The inducible nitric oxide synthase (iNOS) activation in HeLa cells was analyzed using fluorescence microscopy for light microscopic examination. The MCA mice showed a significant increase in plasma MDA, PMN, IL-1β, TNF-α, and nitrates levels. At the same time, the mRNA level of COX-2 in HeLa cells was also significantly increased. These changes were attenuated by crocetin supplementation in the MCA mice. Crocetin supplementation in the MCA mice also showed protection against cervical cancer. These results suggest that crocetin may act as a chemopreventive and an anti-inflammatory agent.

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Crocetin inhibited COX-2 production and expression in HeLa cells. (a) Expression analysis of COX-2 in HeLa cells. Lane U shows untreated HeLa cells; lane T shows crocetin-treated HeLa cells; lane N shows negative control for RT-PCR. β-Actin was used as an internal control. Representative gels from one of the three experiments were used. (b) The mRNA level of COX-2 was measured in HeLa cells treated with various concentrations of crocetin for 24 h. Values represent the mean ± SEM. *P < 0.05 versus control group.
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fig5: Crocetin inhibited COX-2 production and expression in HeLa cells. (a) Expression analysis of COX-2 in HeLa cells. Lane U shows untreated HeLa cells; lane T shows crocetin-treated HeLa cells; lane N shows negative control for RT-PCR. β-Actin was used as an internal control. Representative gels from one of the three experiments were used. (b) The mRNA level of COX-2 was measured in HeLa cells treated with various concentrations of crocetin for 24 h. Values represent the mean ± SEM. *P < 0.05 versus control group.

Mentions: According to a previous research by Subbaramaiah and Dannenberg, COX-2 is involved in inflammation and its upregulation has been reported in various cancers [29]. In the present study, HeLa cells were shown to express high levels of COX-2, which was subsequently downregulated on treatment with crocetin (Figure 5(a)). Relative to nontreated controls, crocetin dose-dependently decreased COX-2 production in cervical cancer cells (Figure 5(b)). It has been suggested that COX-2 is an important target for the chemopreventive effects of these agents. The present study is consistent with this study [30]. Our observations prompt us to suggest that inhibition of COX-2 production by crocetin may suppress growth and invasiveness of cervical carcinomas.


Crocetin downregulates the proinflammatory cytokines in methylcholanthrene-induced rodent tumor model and inhibits COX-2 expression in cervical cancer cells.

Chen B, Hou ZH, Dong Z, Li CD - Biomed Res Int (2015)

Crocetin inhibited COX-2 production and expression in HeLa cells. (a) Expression analysis of COX-2 in HeLa cells. Lane U shows untreated HeLa cells; lane T shows crocetin-treated HeLa cells; lane N shows negative control for RT-PCR. β-Actin was used as an internal control. Representative gels from one of the three experiments were used. (b) The mRNA level of COX-2 was measured in HeLa cells treated with various concentrations of crocetin for 24 h. Values represent the mean ± SEM. *P < 0.05 versus control group.
© Copyright Policy
Related In: Results  -  Collection

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Show All Figures
getmorefigures.php?uid=PMC4385625&req=5

fig5: Crocetin inhibited COX-2 production and expression in HeLa cells. (a) Expression analysis of COX-2 in HeLa cells. Lane U shows untreated HeLa cells; lane T shows crocetin-treated HeLa cells; lane N shows negative control for RT-PCR. β-Actin was used as an internal control. Representative gels from one of the three experiments were used. (b) The mRNA level of COX-2 was measured in HeLa cells treated with various concentrations of crocetin for 24 h. Values represent the mean ± SEM. *P < 0.05 versus control group.
Mentions: According to a previous research by Subbaramaiah and Dannenberg, COX-2 is involved in inflammation and its upregulation has been reported in various cancers [29]. In the present study, HeLa cells were shown to express high levels of COX-2, which was subsequently downregulated on treatment with crocetin (Figure 5(a)). Relative to nontreated controls, crocetin dose-dependently decreased COX-2 production in cervical cancer cells (Figure 5(b)). It has been suggested that COX-2 is an important target for the chemopreventive effects of these agents. The present study is consistent with this study [30]. Our observations prompt us to suggest that inhibition of COX-2 production by crocetin may suppress growth and invasiveness of cervical carcinomas.

Bottom Line: The inducible nitric oxide synthase (iNOS) activation in HeLa cells was analyzed using fluorescence microscopy for light microscopic examination.At the same time, the mRNA level of COX-2 in HeLa cells was also significantly increased.These results suggest that crocetin may act as a chemopreventive and an anti-inflammatory agent.

View Article: PubMed Central - PubMed

Affiliation: Department of Obstetrics and Gynaecology, Air Force General Hospital, PLA, Beijing 100142, China.

ABSTRACT
The effect of crocetin (C20H24O4) on methylcholanthrene- (MCA-) induced uterine cervical cancer in mice was studied in this paper. After the mice were treated orally with crocetin, maleic dialdehyde (MDA), polymorphonuclear cells (PMN), interleukin-1β (IL-1β), and tumor necrosis factor-α (TNF-α) were examined by ELISA or immunohistochemistry. The inducible nitric oxide synthase (iNOS) activation in HeLa cells was analyzed using fluorescence microscopy for light microscopic examination. The MCA mice showed a significant increase in plasma MDA, PMN, IL-1β, TNF-α, and nitrates levels. At the same time, the mRNA level of COX-2 in HeLa cells was also significantly increased. These changes were attenuated by crocetin supplementation in the MCA mice. Crocetin supplementation in the MCA mice also showed protection against cervical cancer. These results suggest that crocetin may act as a chemopreventive and an anti-inflammatory agent.

Show MeSH
Related in: MedlinePlus