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Crocetin downregulates the proinflammatory cytokines in methylcholanthrene-induced rodent tumor model and inhibits COX-2 expression in cervical cancer cells.

Chen B, Hou ZH, Dong Z, Li CD - Biomed Res Int (2015)

Bottom Line: The inducible nitric oxide synthase (iNOS) activation in HeLa cells was analyzed using fluorescence microscopy for light microscopic examination.At the same time, the mRNA level of COX-2 in HeLa cells was also significantly increased.These results suggest that crocetin may act as a chemopreventive and an anti-inflammatory agent.

View Article: PubMed Central - PubMed

Affiliation: Department of Obstetrics and Gynaecology, Air Force General Hospital, PLA, Beijing 100142, China.

ABSTRACT
The effect of crocetin (C20H24O4) on methylcholanthrene- (MCA-) induced uterine cervical cancer in mice was studied in this paper. After the mice were treated orally with crocetin, maleic dialdehyde (MDA), polymorphonuclear cells (PMN), interleukin-1β (IL-1β), and tumor necrosis factor-α (TNF-α) were examined by ELISA or immunohistochemistry. The inducible nitric oxide synthase (iNOS) activation in HeLa cells was analyzed using fluorescence microscopy for light microscopic examination. The MCA mice showed a significant increase in plasma MDA, PMN, IL-1β, TNF-α, and nitrates levels. At the same time, the mRNA level of COX-2 in HeLa cells was also significantly increased. These changes were attenuated by crocetin supplementation in the MCA mice. Crocetin supplementation in the MCA mice also showed protection against cervical cancer. These results suggest that crocetin may act as a chemopreventive and an anti-inflammatory agent.

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Demonstration of iNOS protein in tumor. All photographs were taken at an exposure time of 1 s. Magnification ×400. (a) iNOS in cytoplasm of tumor cells of control rat. (b) iNOS in cytoplasm of tumor cells of IMO rat. (c) iNOS in cytoplasm of tumor cells of crocetin (40 mg/kg) rat.
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fig4: Demonstration of iNOS protein in tumor. All photographs were taken at an exposure time of 1 s. Magnification ×400. (a) iNOS in cytoplasm of tumor cells of control rat. (b) iNOS in cytoplasm of tumor cells of IMO rat. (c) iNOS in cytoplasm of tumor cells of crocetin (40 mg/kg) rat.

Mentions: Inducible NOS is induced in response to inflammatory-like stimuli and is capable of sustained production of high levels of NO that predominate during inflammation [27]. The excessive or inappropriate production of NO can damage tissue through the superoxide anion (O2−) [28]. The fluorescence microscopy analyses showed evidence of widespread iNOS expression in tumor cells from animals with MCA but no evidence of iNOS immunoreactivity in controls. Treatment with crocetin (40 mg/kg) also inhibited the iNOS expression in bone marrow cells (Figure 4).


Crocetin downregulates the proinflammatory cytokines in methylcholanthrene-induced rodent tumor model and inhibits COX-2 expression in cervical cancer cells.

Chen B, Hou ZH, Dong Z, Li CD - Biomed Res Int (2015)

Demonstration of iNOS protein in tumor. All photographs were taken at an exposure time of 1 s. Magnification ×400. (a) iNOS in cytoplasm of tumor cells of control rat. (b) iNOS in cytoplasm of tumor cells of IMO rat. (c) iNOS in cytoplasm of tumor cells of crocetin (40 mg/kg) rat.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4385625&req=5

fig4: Demonstration of iNOS protein in tumor. All photographs were taken at an exposure time of 1 s. Magnification ×400. (a) iNOS in cytoplasm of tumor cells of control rat. (b) iNOS in cytoplasm of tumor cells of IMO rat. (c) iNOS in cytoplasm of tumor cells of crocetin (40 mg/kg) rat.
Mentions: Inducible NOS is induced in response to inflammatory-like stimuli and is capable of sustained production of high levels of NO that predominate during inflammation [27]. The excessive or inappropriate production of NO can damage tissue through the superoxide anion (O2−) [28]. The fluorescence microscopy analyses showed evidence of widespread iNOS expression in tumor cells from animals with MCA but no evidence of iNOS immunoreactivity in controls. Treatment with crocetin (40 mg/kg) also inhibited the iNOS expression in bone marrow cells (Figure 4).

Bottom Line: The inducible nitric oxide synthase (iNOS) activation in HeLa cells was analyzed using fluorescence microscopy for light microscopic examination.At the same time, the mRNA level of COX-2 in HeLa cells was also significantly increased.These results suggest that crocetin may act as a chemopreventive and an anti-inflammatory agent.

View Article: PubMed Central - PubMed

Affiliation: Department of Obstetrics and Gynaecology, Air Force General Hospital, PLA, Beijing 100142, China.

ABSTRACT
The effect of crocetin (C20H24O4) on methylcholanthrene- (MCA-) induced uterine cervical cancer in mice was studied in this paper. After the mice were treated orally with crocetin, maleic dialdehyde (MDA), polymorphonuclear cells (PMN), interleukin-1β (IL-1β), and tumor necrosis factor-α (TNF-α) were examined by ELISA or immunohistochemistry. The inducible nitric oxide synthase (iNOS) activation in HeLa cells was analyzed using fluorescence microscopy for light microscopic examination. The MCA mice showed a significant increase in plasma MDA, PMN, IL-1β, TNF-α, and nitrates levels. At the same time, the mRNA level of COX-2 in HeLa cells was also significantly increased. These changes were attenuated by crocetin supplementation in the MCA mice. Crocetin supplementation in the MCA mice also showed protection against cervical cancer. These results suggest that crocetin may act as a chemopreventive and an anti-inflammatory agent.

Show MeSH
Related in: MedlinePlus