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Crocetin downregulates the proinflammatory cytokines in methylcholanthrene-induced rodent tumor model and inhibits COX-2 expression in cervical cancer cells.

Chen B, Hou ZH, Dong Z, Li CD - Biomed Res Int (2015)

Bottom Line: The inducible nitric oxide synthase (iNOS) activation in HeLa cells was analyzed using fluorescence microscopy for light microscopic examination.At the same time, the mRNA level of COX-2 in HeLa cells was also significantly increased.These results suggest that crocetin may act as a chemopreventive and an anti-inflammatory agent.

View Article: PubMed Central - PubMed

Affiliation: Department of Obstetrics and Gynaecology, Air Force General Hospital, PLA, Beijing 100142, China.

ABSTRACT
The effect of crocetin (C20H24O4) on methylcholanthrene- (MCA-) induced uterine cervical cancer in mice was studied in this paper. After the mice were treated orally with crocetin, maleic dialdehyde (MDA), polymorphonuclear cells (PMN), interleukin-1β (IL-1β), and tumor necrosis factor-α (TNF-α) were examined by ELISA or immunohistochemistry. The inducible nitric oxide synthase (iNOS) activation in HeLa cells was analyzed using fluorescence microscopy for light microscopic examination. The MCA mice showed a significant increase in plasma MDA, PMN, IL-1β, TNF-α, and nitrates levels. At the same time, the mRNA level of COX-2 in HeLa cells was also significantly increased. These changes were attenuated by crocetin supplementation in the MCA mice. Crocetin supplementation in the MCA mice also showed protection against cervical cancer. These results suggest that crocetin may act as a chemopreventive and an anti-inflammatory agent.

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Effect of crocetin on TNF-α concentration. Values represent the mean ± SEM. *P < 0.05 versus MCA group.
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fig3: Effect of crocetin on TNF-α concentration. Values represent the mean ± SEM. *P < 0.05 versus MCA group.

Mentions: Key features of cancer-related inflammation include the infiltration of white blood cells and cytokines such as interleukin (IL)-1, IL-6, and tumor necrosis factor-α (TNF-α) [24]. IL-1β has been shown to be upregulated in many cancers and confers chemoresistance in pancreatic carcinoma [25]. Our study is consistent with these studies. Figure 2 shows that MCA significantly increased protein concentration of IL-1β. Crocetin (40 mg/kg) and crocetin (20 mg/kg) treatment decreased the level of IL-1β by 34% and 56% as compared to the MCA group, respectively (P < 0.05). TNF-α is an inflammatory cytokine which may play important roles in the progression of cervical lesions [26]. TNF-α was measured in blood to evaluate whether the concentration of this cytokine was systemically influenced by crocetin. Furthermore, this helps elucidate whether or not TNF-α can be implicated in the effect that was seen on neutrophil migration. As shown in Figure 3, the levels of TNF-α elevated significantly after induction of tumors with MCA. Crocetin (40 mg/kg) suppressed this response (P < 0.05). We demonstrated in this study that administration of crocetin decreased serum levels of IL-1β and TNF-α that are known to be produced by induction of tumors with MCA. Results from studies on cytokines have given us some insight into the mechanisms involved in the protection of crocetin against cervical cancer (Table 2).


Crocetin downregulates the proinflammatory cytokines in methylcholanthrene-induced rodent tumor model and inhibits COX-2 expression in cervical cancer cells.

Chen B, Hou ZH, Dong Z, Li CD - Biomed Res Int (2015)

Effect of crocetin on TNF-α concentration. Values represent the mean ± SEM. *P < 0.05 versus MCA group.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4385625&req=5

fig3: Effect of crocetin on TNF-α concentration. Values represent the mean ± SEM. *P < 0.05 versus MCA group.
Mentions: Key features of cancer-related inflammation include the infiltration of white blood cells and cytokines such as interleukin (IL)-1, IL-6, and tumor necrosis factor-α (TNF-α) [24]. IL-1β has been shown to be upregulated in many cancers and confers chemoresistance in pancreatic carcinoma [25]. Our study is consistent with these studies. Figure 2 shows that MCA significantly increased protein concentration of IL-1β. Crocetin (40 mg/kg) and crocetin (20 mg/kg) treatment decreased the level of IL-1β by 34% and 56% as compared to the MCA group, respectively (P < 0.05). TNF-α is an inflammatory cytokine which may play important roles in the progression of cervical lesions [26]. TNF-α was measured in blood to evaluate whether the concentration of this cytokine was systemically influenced by crocetin. Furthermore, this helps elucidate whether or not TNF-α can be implicated in the effect that was seen on neutrophil migration. As shown in Figure 3, the levels of TNF-α elevated significantly after induction of tumors with MCA. Crocetin (40 mg/kg) suppressed this response (P < 0.05). We demonstrated in this study that administration of crocetin decreased serum levels of IL-1β and TNF-α that are known to be produced by induction of tumors with MCA. Results from studies on cytokines have given us some insight into the mechanisms involved in the protection of crocetin against cervical cancer (Table 2).

Bottom Line: The inducible nitric oxide synthase (iNOS) activation in HeLa cells was analyzed using fluorescence microscopy for light microscopic examination.At the same time, the mRNA level of COX-2 in HeLa cells was also significantly increased.These results suggest that crocetin may act as a chemopreventive and an anti-inflammatory agent.

View Article: PubMed Central - PubMed

Affiliation: Department of Obstetrics and Gynaecology, Air Force General Hospital, PLA, Beijing 100142, China.

ABSTRACT
The effect of crocetin (C20H24O4) on methylcholanthrene- (MCA-) induced uterine cervical cancer in mice was studied in this paper. After the mice were treated orally with crocetin, maleic dialdehyde (MDA), polymorphonuclear cells (PMN), interleukin-1β (IL-1β), and tumor necrosis factor-α (TNF-α) were examined by ELISA or immunohistochemistry. The inducible nitric oxide synthase (iNOS) activation in HeLa cells was analyzed using fluorescence microscopy for light microscopic examination. The MCA mice showed a significant increase in plasma MDA, PMN, IL-1β, TNF-α, and nitrates levels. At the same time, the mRNA level of COX-2 in HeLa cells was also significantly increased. These changes were attenuated by crocetin supplementation in the MCA mice. Crocetin supplementation in the MCA mice also showed protection against cervical cancer. These results suggest that crocetin may act as a chemopreventive and an anti-inflammatory agent.

Show MeSH
Related in: MedlinePlus