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Crocetin downregulates the proinflammatory cytokines in methylcholanthrene-induced rodent tumor model and inhibits COX-2 expression in cervical cancer cells.

Chen B, Hou ZH, Dong Z, Li CD - Biomed Res Int (2015)

Bottom Line: The inducible nitric oxide synthase (iNOS) activation in HeLa cells was analyzed using fluorescence microscopy for light microscopic examination.At the same time, the mRNA level of COX-2 in HeLa cells was also significantly increased.These results suggest that crocetin may act as a chemopreventive and an anti-inflammatory agent.

View Article: PubMed Central - PubMed

Affiliation: Department of Obstetrics and Gynaecology, Air Force General Hospital, PLA, Beijing 100142, China.

ABSTRACT
The effect of crocetin (C20H24O4) on methylcholanthrene- (MCA-) induced uterine cervical cancer in mice was studied in this paper. After the mice were treated orally with crocetin, maleic dialdehyde (MDA), polymorphonuclear cells (PMN), interleukin-1β (IL-1β), and tumor necrosis factor-α (TNF-α) were examined by ELISA or immunohistochemistry. The inducible nitric oxide synthase (iNOS) activation in HeLa cells was analyzed using fluorescence microscopy for light microscopic examination. The MCA mice showed a significant increase in plasma MDA, PMN, IL-1β, TNF-α, and nitrates levels. At the same time, the mRNA level of COX-2 in HeLa cells was also significantly increased. These changes were attenuated by crocetin supplementation in the MCA mice. Crocetin supplementation in the MCA mice also showed protection against cervical cancer. These results suggest that crocetin may act as a chemopreventive and an anti-inflammatory agent.

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Effect of crocetin on MDA level. Values represent the mean ± SEM. *P < 0.05 versus MCA group. **P < 0.01 versus MCA group.
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fig1: Effect of crocetin on MDA level. Values represent the mean ± SEM. *P < 0.05 versus MCA group. **P < 0.01 versus MCA group.

Mentions: Peroxidation damage plays an important role in the progression of inflammation mediated diseases in particular cervical cancer. The central dogma in chronic inflammation hypothesis emphasizes the role of ROS generated by phagocytes, which cause cytotoxicity and mutagenesis [15–17]. Therefore, the antioxidant effects of crocetin were investigated by measuring MDA levels. The control animals showed low MDA levels; however, the MDA levels in the MCA group were significantly higher (P < 0.05). As shown in Figure 1, MDA levels in the crocetin (40 mg/kg) and crocetin (20 mg/kg) groups were significantly lower than those in the MCA group (P < 0.01 and P < 0.05, resp.). This result is in agreement with previous reports [18, 19]. It suggests that crocetin might exert a profound effect on inhibition of lipid peroxidation and free radical generation.


Crocetin downregulates the proinflammatory cytokines in methylcholanthrene-induced rodent tumor model and inhibits COX-2 expression in cervical cancer cells.

Chen B, Hou ZH, Dong Z, Li CD - Biomed Res Int (2015)

Effect of crocetin on MDA level. Values represent the mean ± SEM. *P < 0.05 versus MCA group. **P < 0.01 versus MCA group.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4385625&req=5

fig1: Effect of crocetin on MDA level. Values represent the mean ± SEM. *P < 0.05 versus MCA group. **P < 0.01 versus MCA group.
Mentions: Peroxidation damage plays an important role in the progression of inflammation mediated diseases in particular cervical cancer. The central dogma in chronic inflammation hypothesis emphasizes the role of ROS generated by phagocytes, which cause cytotoxicity and mutagenesis [15–17]. Therefore, the antioxidant effects of crocetin were investigated by measuring MDA levels. The control animals showed low MDA levels; however, the MDA levels in the MCA group were significantly higher (P < 0.05). As shown in Figure 1, MDA levels in the crocetin (40 mg/kg) and crocetin (20 mg/kg) groups were significantly lower than those in the MCA group (P < 0.01 and P < 0.05, resp.). This result is in agreement with previous reports [18, 19]. It suggests that crocetin might exert a profound effect on inhibition of lipid peroxidation and free radical generation.

Bottom Line: The inducible nitric oxide synthase (iNOS) activation in HeLa cells was analyzed using fluorescence microscopy for light microscopic examination.At the same time, the mRNA level of COX-2 in HeLa cells was also significantly increased.These results suggest that crocetin may act as a chemopreventive and an anti-inflammatory agent.

View Article: PubMed Central - PubMed

Affiliation: Department of Obstetrics and Gynaecology, Air Force General Hospital, PLA, Beijing 100142, China.

ABSTRACT
The effect of crocetin (C20H24O4) on methylcholanthrene- (MCA-) induced uterine cervical cancer in mice was studied in this paper. After the mice were treated orally with crocetin, maleic dialdehyde (MDA), polymorphonuclear cells (PMN), interleukin-1β (IL-1β), and tumor necrosis factor-α (TNF-α) were examined by ELISA or immunohistochemistry. The inducible nitric oxide synthase (iNOS) activation in HeLa cells was analyzed using fluorescence microscopy for light microscopic examination. The MCA mice showed a significant increase in plasma MDA, PMN, IL-1β, TNF-α, and nitrates levels. At the same time, the mRNA level of COX-2 in HeLa cells was also significantly increased. These changes were attenuated by crocetin supplementation in the MCA mice. Crocetin supplementation in the MCA mice also showed protection against cervical cancer. These results suggest that crocetin may act as a chemopreventive and an anti-inflammatory agent.

Show MeSH
Related in: MedlinePlus