Limits...
Osteoprotective effect of cordycepin on estrogen deficiency-induced osteoporosis in vitro and in vivo.

Zhang DW, Deng H, Qi W, Zhao GY, Cao XR - Biomed Res Int (2015)

Bottom Line: At the same time, we further investigated the effect of cordycepin in vitro assay.The beneficial effects of cordycepin on improvement of osteoporosis in rats were attributable mainly to decrease ALP activity, TRAP activity, and CTX level.These experimental results suggest that complement cordycepin is protective after ovariectomized osteopenic in specific way.

View Article: PubMed Central - PubMed

Affiliation: Department of Orthopedics, Xi Jing Hospital, The Fourth Military Medical University, Xi'an 710032, China.

ABSTRACT
The purpose of this study was to verify the effect of cordycepin on ovariectomized osteopenic rats. Fifty Wistar female rats used were divided into 5 groups: (1) sham-operation rats (control), (2) ovariectomized (OVX) rats with osteopenia, and (3) OVX'd rats with osteopenia treated with cordycepin (5 mg, 10 mg, and 20 mg) for 8 weeks. After the rats were treated orally with cordycepin, serum alkaline phosphatase (ALP), tartrate resistant acid phosphatase (TRAP), serum osteocalcin (OC), homocysteine (HCY) , C-terminal crosslinked telopeptides of collagen type I (CTX) level, and oxidative stress were examined, respectively. The femoral neck was used for mechanical compression testing. At the same time, we further investigated the effect of cordycepin in vitro assay. The beneficial effects of cordycepin on improvement of osteoporosis in rats were attributable mainly to decrease ALP activity, TRAP activity, and CTX level. At the same time, cordycepin also increases the OC level in ovariectomized osteopenic rats. The histological examination clearly showed that dietary cordycepin can prevent bone loss caused by estrogen deficiency. These experimental results suggest that complement cordycepin is protective after ovariectomized osteopenic in specific way.

Show MeSH

Related in: MedlinePlus

Histology of lumbar vertebrae. The bone structure was photographed under a light microscope. It shows that there was a significant trabecular bone loss in the OVX rat (b), whereas the cordycepin-20 treatment rat section (c) seems near normal compared with sham-operated animals (a).
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4385597&req=5

fig4: Histology of lumbar vertebrae. The bone structure was photographed under a light microscope. It shows that there was a significant trabecular bone loss in the OVX rat (b), whereas the cordycepin-20 treatment rat section (c) seems near normal compared with sham-operated animals (a).

Mentions: Bone histomorphometry was also performed to determine the effects of cordycepin treatment on cancellous bone mass and levels of bone formation and resorption. A sample photomicrograph is presented in Figure 4. It is quite clear that trabecular bone loss is much higher in the vertebrae of rat with OVX (Figure 4(b)), whereas the vertebrae of cordycepin-fed OVX rat (Figure 4(a)) appear to be near normal (Figure 4(c)).


Osteoprotective effect of cordycepin on estrogen deficiency-induced osteoporosis in vitro and in vivo.

Zhang DW, Deng H, Qi W, Zhao GY, Cao XR - Biomed Res Int (2015)

Histology of lumbar vertebrae. The bone structure was photographed under a light microscope. It shows that there was a significant trabecular bone loss in the OVX rat (b), whereas the cordycepin-20 treatment rat section (c) seems near normal compared with sham-operated animals (a).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4385597&req=5

fig4: Histology of lumbar vertebrae. The bone structure was photographed under a light microscope. It shows that there was a significant trabecular bone loss in the OVX rat (b), whereas the cordycepin-20 treatment rat section (c) seems near normal compared with sham-operated animals (a).
Mentions: Bone histomorphometry was also performed to determine the effects of cordycepin treatment on cancellous bone mass and levels of bone formation and resorption. A sample photomicrograph is presented in Figure 4. It is quite clear that trabecular bone loss is much higher in the vertebrae of rat with OVX (Figure 4(b)), whereas the vertebrae of cordycepin-fed OVX rat (Figure 4(a)) appear to be near normal (Figure 4(c)).

Bottom Line: At the same time, we further investigated the effect of cordycepin in vitro assay.The beneficial effects of cordycepin on improvement of osteoporosis in rats were attributable mainly to decrease ALP activity, TRAP activity, and CTX level.These experimental results suggest that complement cordycepin is protective after ovariectomized osteopenic in specific way.

View Article: PubMed Central - PubMed

Affiliation: Department of Orthopedics, Xi Jing Hospital, The Fourth Military Medical University, Xi'an 710032, China.

ABSTRACT
The purpose of this study was to verify the effect of cordycepin on ovariectomized osteopenic rats. Fifty Wistar female rats used were divided into 5 groups: (1) sham-operation rats (control), (2) ovariectomized (OVX) rats with osteopenia, and (3) OVX'd rats with osteopenia treated with cordycepin (5 mg, 10 mg, and 20 mg) for 8 weeks. After the rats were treated orally with cordycepin, serum alkaline phosphatase (ALP), tartrate resistant acid phosphatase (TRAP), serum osteocalcin (OC), homocysteine (HCY) , C-terminal crosslinked telopeptides of collagen type I (CTX) level, and oxidative stress were examined, respectively. The femoral neck was used for mechanical compression testing. At the same time, we further investigated the effect of cordycepin in vitro assay. The beneficial effects of cordycepin on improvement of osteoporosis in rats were attributable mainly to decrease ALP activity, TRAP activity, and CTX level. At the same time, cordycepin also increases the OC level in ovariectomized osteopenic rats. The histological examination clearly showed that dietary cordycepin can prevent bone loss caused by estrogen deficiency. These experimental results suggest that complement cordycepin is protective after ovariectomized osteopenic in specific way.

Show MeSH
Related in: MedlinePlus