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Immunogenicity comparison of conjugate vaccines composed of alginate and lipopolysaccharide of Pseudomonas aeruginosa bound to diphtheria toxoid.

Najafzadeh F, Jaberi G, Shapouri R, Rahnema M, Karimi-Nik A, Kianmehr A - Iran J Microbiol (2014)

Bottom Line: The resulting depolymerized alginate (D-ALG) and detoxified LPS (D-LPS) were covalently coupled to diphtheria toxoid (DT) as a carrier protein with adipic acid dihydrazide (ADH) as a spacer molecule and carbodiimide as a linker.The conjugates were non-toxic and non-pyrogenic.ELISA results indicated that antibodies titer of D-ALGDT was more than D-LPSDT.

View Article: PubMed Central - PubMed

Affiliation: Department of Microbiology, Zanjan Branch, Islamic Azad University, Zanjan, Iran.

ABSTRACT

Background and objectives: Treatment of Pseudomonas aeruginosa infections is greatly hampered by innate and acquired antibiotic resistance. The goal of this study was to compure the immunogenicity of conjugates of P. aeruginosa depolymerized alginate-diphtheria toxoid (D-ALGDT) and P. aeruginosa detoxified lipopolysaccharidediphtheria toxoid (D-LPSDT) in mouse model.

Materials and methods: Alginate and LPS were purified from P. aeruginosa strain PAO1. The resulting depolymerized alginate (D-ALG) and detoxified LPS (D-LPS) were covalently coupled to diphtheria toxoid (DT) as a carrier protein with adipic acid dihydrazide (ADH) as a spacer molecule and carbodiimide as a linker. Sterility, safety and pyrogenicity tests were performed. 30 mice in two groups were immunized intraperitoneally on days 0, 14 and 28 with 10 μg of D-ALGDT and D-LPSDT. Conjugates specific antibody levels were also determined by enzyme-linked immunosorbent assay (ELISA).

Results: The conjugates were non-toxic and non-pyrogenic. Conjugates of D-ALGDT and D-LPSDT were shown to be safe and to elicit total IgG, IgM, IgA, IgG1, IgG2a, IgG2b and IgG3 antibodies in mice. ELISA results indicated that antibodies titer of D-ALGDT was more than D-LPSDT.

Conclusion: Immunization with D-ALGDT showed significant increase in all types of antibodies titers in versus D-LPSDT, suggesting D-ALGDT as a vaccine candidate against P. aeruginosa infections.

No MeSH data available.


Related in: MedlinePlus

Induction of antibodies in BALB/c mice for two weeks after first injection (Day 14). The results of inductions for all types of antibodies were observed D-ALG-DT>D-LPS-DT.
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Figure 4: Induction of antibodies in BALB/c mice for two weeks after first injection (Day 14). The results of inductions for all types of antibodies were observed D-ALG-DT>D-LPS-DT.

Mentions: The immunogenicity of the D-LPS-DT and D-ALG-DT conjugate vaccines were analyzed by immunization in mice. Figs. 4, 5 and 6 shows antibody titers in the immunized mice. As shown in these figures, D-ALG-DT displayed higher titers in the IgG and IgM antibodies than D-LPS-DT (P<0.01). The control groups also indicated the lowest antibody titers.


Immunogenicity comparison of conjugate vaccines composed of alginate and lipopolysaccharide of Pseudomonas aeruginosa bound to diphtheria toxoid.

Najafzadeh F, Jaberi G, Shapouri R, Rahnema M, Karimi-Nik A, Kianmehr A - Iran J Microbiol (2014)

Induction of antibodies in BALB/c mice for two weeks after first injection (Day 14). The results of inductions for all types of antibodies were observed D-ALG-DT>D-LPS-DT.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4385571&req=5

Figure 4: Induction of antibodies in BALB/c mice for two weeks after first injection (Day 14). The results of inductions for all types of antibodies were observed D-ALG-DT>D-LPS-DT.
Mentions: The immunogenicity of the D-LPS-DT and D-ALG-DT conjugate vaccines were analyzed by immunization in mice. Figs. 4, 5 and 6 shows antibody titers in the immunized mice. As shown in these figures, D-ALG-DT displayed higher titers in the IgG and IgM antibodies than D-LPS-DT (P<0.01). The control groups also indicated the lowest antibody titers.

Bottom Line: The resulting depolymerized alginate (D-ALG) and detoxified LPS (D-LPS) were covalently coupled to diphtheria toxoid (DT) as a carrier protein with adipic acid dihydrazide (ADH) as a spacer molecule and carbodiimide as a linker.The conjugates were non-toxic and non-pyrogenic.ELISA results indicated that antibodies titer of D-ALGDT was more than D-LPSDT.

View Article: PubMed Central - PubMed

Affiliation: Department of Microbiology, Zanjan Branch, Islamic Azad University, Zanjan, Iran.

ABSTRACT

Background and objectives: Treatment of Pseudomonas aeruginosa infections is greatly hampered by innate and acquired antibiotic resistance. The goal of this study was to compure the immunogenicity of conjugates of P. aeruginosa depolymerized alginate-diphtheria toxoid (D-ALGDT) and P. aeruginosa detoxified lipopolysaccharidediphtheria toxoid (D-LPSDT) in mouse model.

Materials and methods: Alginate and LPS were purified from P. aeruginosa strain PAO1. The resulting depolymerized alginate (D-ALG) and detoxified LPS (D-LPS) were covalently coupled to diphtheria toxoid (DT) as a carrier protein with adipic acid dihydrazide (ADH) as a spacer molecule and carbodiimide as a linker. Sterility, safety and pyrogenicity tests were performed. 30 mice in two groups were immunized intraperitoneally on days 0, 14 and 28 with 10 μg of D-ALGDT and D-LPSDT. Conjugates specific antibody levels were also determined by enzyme-linked immunosorbent assay (ELISA).

Results: The conjugates were non-toxic and non-pyrogenic. Conjugates of D-ALGDT and D-LPSDT were shown to be safe and to elicit total IgG, IgM, IgA, IgG1, IgG2a, IgG2b and IgG3 antibodies in mice. ELISA results indicated that antibodies titer of D-ALGDT was more than D-LPSDT.

Conclusion: Immunization with D-ALGDT showed significant increase in all types of antibodies titers in versus D-LPSDT, suggesting D-ALGDT as a vaccine candidate against P. aeruginosa infections.

No MeSH data available.


Related in: MedlinePlus