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Prevalence assessment of magA gene and antimicrobial susceptibility of Klebsiella pneumoniae isolated from clinical specimens in Shahrekord, Iran.

Amraie H, Shakib P, Rouhi S, Bakhshandeh N, Zamanzad B - Iran J Microbiol (2014)

Bottom Line: The highest and lowest rates of resistance were related to amoxicillin 79.19% and ciprofloxacin 15.60%, respectively.Also 4 samples were positive for magA gene.Based on our results, K. pneumoniae strains were resistant to different antibiotics.

View Article: PubMed Central - PubMed

Affiliation: Department of Microbiology, Lorestan University of Medical Sciences, Khorramabad, Iran.

ABSTRACT

Background and objectives: Klebsiella pneumoniae (K. pneumoniae) is an opportunistic microorganism. This study aimed to investigate the presence of magA gene and antimicrobial susceptibility in K. pneumoniae.

Materials and methods: clinical specimens were collected from hospitals of Shahrekord, Iran. Bacterial culture, biochemical diagnostic standard test, determination of antibiotic sensitivity, phenotypic testing hypermucoviscosity (HV) and polymerase chain reaction (PCR) was performed for isolation and characterization of K. pneumoniae.

Results: 173 samples were positive for K. pneumoniae. The highest and lowest rates of resistance were related to amoxicillin 79.19% and ciprofloxacin 15.60%, respectively. Also 4 samples were positive for magA gene.

Conclusion: Based on our results, K. pneumoniae strains were resistant to different antibiotics. Knowing how to identify strains of K. pneumoniae, spreading of its virulence and also antimicrobial resistance genes can be useful in treatment of infection caused by this bacterium.

No MeSH data available.


Related in: MedlinePlus

Result of the PCR assay for identification of K. pneumoniae magA and genes blaSHV-1agenesM, marker 100 bpN, negative controlNumber 1, positive control strains of K. pneumoniaeNumbers 1 to 8, isolated K. pneumoniae with blaSHV-1a geneNumbers 2 to 8, isolated K. pneumoniae from patientsNumbers 2, 3, 4, 6, 7 and 8, isolated K. pneumoniae without magA geneNumber 5, isolated K. pneumoniae with magA gene
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Figure 1: Result of the PCR assay for identification of K. pneumoniae magA and genes blaSHV-1agenesM, marker 100 bpN, negative controlNumber 1, positive control strains of K. pneumoniaeNumbers 1 to 8, isolated K. pneumoniae with blaSHV-1a geneNumbers 2 to 8, isolated K. pneumoniae from patientsNumbers 2, 3, 4, 6, 7 and 8, isolated K. pneumoniae without magA geneNumber 5, isolated K. pneumoniae with magA gene

Mentions: The presence of magA gene was investigated among 173 of K. pneumoniae isolates. Of 173 isolates of K. pneumoniae, 4 (2.31%) were positive and 169 (97.68%) were negative for magA gene (Fig.1).


Prevalence assessment of magA gene and antimicrobial susceptibility of Klebsiella pneumoniae isolated from clinical specimens in Shahrekord, Iran.

Amraie H, Shakib P, Rouhi S, Bakhshandeh N, Zamanzad B - Iran J Microbiol (2014)

Result of the PCR assay for identification of K. pneumoniae magA and genes blaSHV-1agenesM, marker 100 bpN, negative controlNumber 1, positive control strains of K. pneumoniaeNumbers 1 to 8, isolated K. pneumoniae with blaSHV-1a geneNumbers 2 to 8, isolated K. pneumoniae from patientsNumbers 2, 3, 4, 6, 7 and 8, isolated K. pneumoniae without magA geneNumber 5, isolated K. pneumoniae with magA gene
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4385570&req=5

Figure 1: Result of the PCR assay for identification of K. pneumoniae magA and genes blaSHV-1agenesM, marker 100 bpN, negative controlNumber 1, positive control strains of K. pneumoniaeNumbers 1 to 8, isolated K. pneumoniae with blaSHV-1a geneNumbers 2 to 8, isolated K. pneumoniae from patientsNumbers 2, 3, 4, 6, 7 and 8, isolated K. pneumoniae without magA geneNumber 5, isolated K. pneumoniae with magA gene
Mentions: The presence of magA gene was investigated among 173 of K. pneumoniae isolates. Of 173 isolates of K. pneumoniae, 4 (2.31%) were positive and 169 (97.68%) were negative for magA gene (Fig.1).

Bottom Line: The highest and lowest rates of resistance were related to amoxicillin 79.19% and ciprofloxacin 15.60%, respectively.Also 4 samples were positive for magA gene.Based on our results, K. pneumoniae strains were resistant to different antibiotics.

View Article: PubMed Central - PubMed

Affiliation: Department of Microbiology, Lorestan University of Medical Sciences, Khorramabad, Iran.

ABSTRACT

Background and objectives: Klebsiella pneumoniae (K. pneumoniae) is an opportunistic microorganism. This study aimed to investigate the presence of magA gene and antimicrobial susceptibility in K. pneumoniae.

Materials and methods: clinical specimens were collected from hospitals of Shahrekord, Iran. Bacterial culture, biochemical diagnostic standard test, determination of antibiotic sensitivity, phenotypic testing hypermucoviscosity (HV) and polymerase chain reaction (PCR) was performed for isolation and characterization of K. pneumoniae.

Results: 173 samples were positive for K. pneumoniae. The highest and lowest rates of resistance were related to amoxicillin 79.19% and ciprofloxacin 15.60%, respectively. Also 4 samples were positive for magA gene.

Conclusion: Based on our results, K. pneumoniae strains were resistant to different antibiotics. Knowing how to identify strains of K. pneumoniae, spreading of its virulence and also antimicrobial resistance genes can be useful in treatment of infection caused by this bacterium.

No MeSH data available.


Related in: MedlinePlus