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Effect of single-walled carbon nanotubes on tumor cells viability and formation of multicellular tumor spheroids.

Yakymchuk OM, Perepelytsina OM, Dobrydnev AV, Sydorenko MV - Nanoscale Res Lett (2015)

Bottom Line: Our results demonstrated that SWCNTs at concentrations ranging from 12.5 to 50 μg/ml did not have cytotoxic influence on tumor cells; instead, they had weak cytostatic effect.The result of this influence was in formation of more MTS in cell culture with SWCNTs compared with UDDs and control samples.Our results could be useful for the control of cell growth in three-dimensional culture. 61. 46 + w; 61.48 + c; 61.48De; 87.15-v; 87.64-t.

View Article: PubMed Central - PubMed

Affiliation: Department for Biotechnical Problems of Diagnostic, Institute for Problems of Cryobiology and Cryomedicine of NAS Ukraine, 42/1 Nauky str., 03028 Kiev, Ukraine.

ABSTRACT

Abstract: This paper describes the impact of different concentrations of single-walled carbon nanotubes (SWCNTs) on cell viability of breast adenocarcinoma, MCF-7 line, and formation of multicellular tumor spheroids (MTS). Chemical composition and purity of nanotubes is controlled by Fourier transform infrared spectroscopy. The strength and direction of the influence of SWCNTs on the tumor cell population was assessed by cell counting and measurement of the volume of multicellular tumor spheroids. Effect of SWCNTs on the formation of multicellular spheroids was compared with the results obtained by culturing tumor cells with ultra dispersed diamonds (UDDs). Our results demonstrated that SWCNTs at concentrations ranging from 12.5 to 50 μg/ml did not have cytotoxic influence on tumor cells; instead, they had weak cytostatic effect. The increasing of SWCNTs concentration to 100 to 200 μg/ml stimulated proliferation of tumor cells, especially in suspension fractions. The result of this influence was in formation of more MTS in cell culture with SWCNTs compared with UDDs and control samples. In result, the median volume of MTS after cultivation with SWCNTs at 100 to 200 μg/ml concentrations is 3 to 5 times greater than that in samples which were incubated with the UDDs and is 2.5 times greater than that in control cultures. So, if SWCNTs reduced cell adhesion to substrate and stimulated formation of tumor cell aggregates volume near 7 · 10(-3) mm(3), at the same time, UDDs reduced adhesion and cohesive ability of cells and stimulated generation of cell spheroids volume no more than 4 · 10(-3) mm(3). Our results could be useful for the control of cell growth in three-dimensional culture.

Pacs: 61. 46 + w; 61.48 + c; 61.48De; 87.15-v; 87.64-t.

No MeSH data available.


Related in: MedlinePlus

FTIR spectra of SWCNTs.
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Fig2: FTIR spectra of SWCNTs.

Mentions: The results of SWCNTs FTIR assay are presented in Figure 2. The main features of SWCNTs (Table 1, Figure 2) correspond to the groups O-H stretched (3,600 to 3,200 cm−1). The amino N-H groups showed a strong signal at 3,431 and 3,411 cm−1. In SWCNTs samples, these were not vibration peaks of acid O-H (at 2,924 to 2,913 cm−1), alkane C-H (at 2,845 to 2,851 cm−1), and alkene = C-H groups (675 to 1,000 cm−1). Also, there was no absorption of the alkene C = C covalent band vibrations, which were fixed at 1,619 and 1,653 cm−1. Instead, there was quite intense peaks of acid C = O linkages in the region of 1,717 cm−1; it indicated the presence of carboxyl groups. Some C = O groups can emerge from adsorbed CO and CO2 (at 1,717 cm−1) in SWCNTs specters. The appearance of vibration at 1,400 cm−1 was the evidence of presence in SWCNTs samples of aromatic C = C carbon. Weak signals in the area of wave number 1,025 cm−1 were observed for CNT, which can be attributed to the presence of a small fraction of ether/ester C-O groups (Figure 2). In addition, according to the obtained spectra, SWCNTs have a small amount of absorbed or covalent chemical additives. Our spectra were similar to those presented by other authors [18,19], which allowed us to conclude that compliance with the chemical structure of the samples; their proper degree of purification are relevant for use in further biological studies.Figure 2


Effect of single-walled carbon nanotubes on tumor cells viability and formation of multicellular tumor spheroids.

Yakymchuk OM, Perepelytsina OM, Dobrydnev AV, Sydorenko MV - Nanoscale Res Lett (2015)

FTIR spectra of SWCNTs.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4385323&req=5

Fig2: FTIR spectra of SWCNTs.
Mentions: The results of SWCNTs FTIR assay are presented in Figure 2. The main features of SWCNTs (Table 1, Figure 2) correspond to the groups O-H stretched (3,600 to 3,200 cm−1). The amino N-H groups showed a strong signal at 3,431 and 3,411 cm−1. In SWCNTs samples, these were not vibration peaks of acid O-H (at 2,924 to 2,913 cm−1), alkane C-H (at 2,845 to 2,851 cm−1), and alkene = C-H groups (675 to 1,000 cm−1). Also, there was no absorption of the alkene C = C covalent band vibrations, which were fixed at 1,619 and 1,653 cm−1. Instead, there was quite intense peaks of acid C = O linkages in the region of 1,717 cm−1; it indicated the presence of carboxyl groups. Some C = O groups can emerge from adsorbed CO and CO2 (at 1,717 cm−1) in SWCNTs specters. The appearance of vibration at 1,400 cm−1 was the evidence of presence in SWCNTs samples of aromatic C = C carbon. Weak signals in the area of wave number 1,025 cm−1 were observed for CNT, which can be attributed to the presence of a small fraction of ether/ester C-O groups (Figure 2). In addition, according to the obtained spectra, SWCNTs have a small amount of absorbed or covalent chemical additives. Our spectra were similar to those presented by other authors [18,19], which allowed us to conclude that compliance with the chemical structure of the samples; their proper degree of purification are relevant for use in further biological studies.Figure 2

Bottom Line: Our results demonstrated that SWCNTs at concentrations ranging from 12.5 to 50 μg/ml did not have cytotoxic influence on tumor cells; instead, they had weak cytostatic effect.The result of this influence was in formation of more MTS in cell culture with SWCNTs compared with UDDs and control samples.Our results could be useful for the control of cell growth in three-dimensional culture. 61. 46 + w; 61.48 + c; 61.48De; 87.15-v; 87.64-t.

View Article: PubMed Central - PubMed

Affiliation: Department for Biotechnical Problems of Diagnostic, Institute for Problems of Cryobiology and Cryomedicine of NAS Ukraine, 42/1 Nauky str., 03028 Kiev, Ukraine.

ABSTRACT

Abstract: This paper describes the impact of different concentrations of single-walled carbon nanotubes (SWCNTs) on cell viability of breast adenocarcinoma, MCF-7 line, and formation of multicellular tumor spheroids (MTS). Chemical composition and purity of nanotubes is controlled by Fourier transform infrared spectroscopy. The strength and direction of the influence of SWCNTs on the tumor cell population was assessed by cell counting and measurement of the volume of multicellular tumor spheroids. Effect of SWCNTs on the formation of multicellular spheroids was compared with the results obtained by culturing tumor cells with ultra dispersed diamonds (UDDs). Our results demonstrated that SWCNTs at concentrations ranging from 12.5 to 50 μg/ml did not have cytotoxic influence on tumor cells; instead, they had weak cytostatic effect. The increasing of SWCNTs concentration to 100 to 200 μg/ml stimulated proliferation of tumor cells, especially in suspension fractions. The result of this influence was in formation of more MTS in cell culture with SWCNTs compared with UDDs and control samples. In result, the median volume of MTS after cultivation with SWCNTs at 100 to 200 μg/ml concentrations is 3 to 5 times greater than that in samples which were incubated with the UDDs and is 2.5 times greater than that in control cultures. So, if SWCNTs reduced cell adhesion to substrate and stimulated formation of tumor cell aggregates volume near 7 · 10(-3) mm(3), at the same time, UDDs reduced adhesion and cohesive ability of cells and stimulated generation of cell spheroids volume no more than 4 · 10(-3) mm(3). Our results could be useful for the control of cell growth in three-dimensional culture.

Pacs: 61. 46 + w; 61.48 + c; 61.48De; 87.15-v; 87.64-t.

No MeSH data available.


Related in: MedlinePlus