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Effect of single-walled carbon nanotubes on tumor cells viability and formation of multicellular tumor spheroids.

Yakymchuk OM, Perepelytsina OM, Dobrydnev AV, Sydorenko MV - Nanoscale Res Lett (2015)

Bottom Line: Our results demonstrated that SWCNTs at concentrations ranging from 12.5 to 50 μg/ml did not have cytotoxic influence on tumor cells; instead, they had weak cytostatic effect.The result of this influence was in formation of more MTS in cell culture with SWCNTs compared with UDDs and control samples.Our results could be useful for the control of cell growth in three-dimensional culture. 61. 46 + w; 61.48 + c; 61.48De; 87.15-v; 87.64-t.

View Article: PubMed Central - PubMed

Affiliation: Department for Biotechnical Problems of Diagnostic, Institute for Problems of Cryobiology and Cryomedicine of NAS Ukraine, 42/1 Nauky str., 03028 Kiev, Ukraine.

ABSTRACT

Abstract: This paper describes the impact of different concentrations of single-walled carbon nanotubes (SWCNTs) on cell viability of breast adenocarcinoma, MCF-7 line, and formation of multicellular tumor spheroids (MTS). Chemical composition and purity of nanotubes is controlled by Fourier transform infrared spectroscopy. The strength and direction of the influence of SWCNTs on the tumor cell population was assessed by cell counting and measurement of the volume of multicellular tumor spheroids. Effect of SWCNTs on the formation of multicellular spheroids was compared with the results obtained by culturing tumor cells with ultra dispersed diamonds (UDDs). Our results demonstrated that SWCNTs at concentrations ranging from 12.5 to 50 μg/ml did not have cytotoxic influence on tumor cells; instead, they had weak cytostatic effect. The increasing of SWCNTs concentration to 100 to 200 μg/ml stimulated proliferation of tumor cells, especially in suspension fractions. The result of this influence was in formation of more MTS in cell culture with SWCNTs compared with UDDs and control samples. In result, the median volume of MTS after cultivation with SWCNTs at 100 to 200 μg/ml concentrations is 3 to 5 times greater than that in samples which were incubated with the UDDs and is 2.5 times greater than that in control cultures. So, if SWCNTs reduced cell adhesion to substrate and stimulated formation of tumor cell aggregates volume near 7 · 10(-3) mm(3), at the same time, UDDs reduced adhesion and cohesive ability of cells and stimulated generation of cell spheroids volume no more than 4 · 10(-3) mm(3). Our results could be useful for the control of cell growth in three-dimensional culture.

Pacs: 61. 46 + w; 61.48 + c; 61.48De; 87.15-v; 87.64-t.

No MeSH data available.


Related in: MedlinePlus

Three-dimensional structure of the carbon nanotubes according to the literature[31,32].
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Fig1: Three-dimensional structure of the carbon nanotubes according to the literature[31,32].

Mentions: Single-walled carbon nanotubes (SWCNTs) are a unique structure. SWCNTs are usually produced by twisting two hexagonal fibers of graphite without seams. Nanotubes come in different forms: single-walled, multiwalled, straight, and spiral, with opened and closed ends. SWCNTs have a lot of unique properties. First, it has a high mechanical strength, which is a hundred times more strength than steel and provides the possibility to use it as probes in scanning tunneling microscopy. Nanotubes are extremely durable material like tensile and bending. Moreover, under the influence of mechanical stress exceeding the critical, nanotubes do not ‘rush’ and are tunable [1]. The nanotubes can be obtained simultaneously as a rugged and elastic tissue. The electrical properties of nanotubes are determined by their chirality [2,3]. Depending on chirality, single-walled tube can exhibit properties as graphite - semimetal [4,5]. Due to the small size of carbon nanotubes, the measurement of their electrical resistivity by four-pin way was conducted as early as in 1996. The resistivity of nanotubes can vary in considerable range. The minimal resistivity of nanotubes is much lower than that for graphite. Most of the nanotubes have metallic conductivity and properties of semiconductor with band gap from 0.1 to 0.3 eV (Figure 1). The researchers also opened another property of SWCNTs such as superconductivity [6]. Unique physical and chemical properties of nanotubes are complemented by extremely interesting biological properties. Carbon nanotubes (CNTs) have great potential for application in biology and medicine for bioimaging, targeted delivery vehicles for drugs, plasmid DNA, or small interfering RNA into cells by endocytosis and biosensors, and for mimicking extracellular matrix in tissue regeneration biotechnology. There are many efforts to employ CNTs as tool for anticancer therapy. In this field, carbon nanotubes were subject for fundamental works in cell biology. But there are a lot of unclear aspects of the interaction of nanotubes with tumor cells. Toxicity and carcinogenicity has been one of the major concerns for CNTs’ use in biomedical application. Therefore, in our study, we were looking for answers on a few questions. How do carbon nanotubes affect the survival of tumor cells in monolayer culture? Which properties of nanomaterial determine its relationship with the cells? Finally, which properties of nanotubes can be used in tissue biotechnology? To answer these questions, we chose a model of multicellular tumor spheroids.Figure 1


Effect of single-walled carbon nanotubes on tumor cells viability and formation of multicellular tumor spheroids.

Yakymchuk OM, Perepelytsina OM, Dobrydnev AV, Sydorenko MV - Nanoscale Res Lett (2015)

Three-dimensional structure of the carbon nanotubes according to the literature[31,32].
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4385323&req=5

Fig1: Three-dimensional structure of the carbon nanotubes according to the literature[31,32].
Mentions: Single-walled carbon nanotubes (SWCNTs) are a unique structure. SWCNTs are usually produced by twisting two hexagonal fibers of graphite without seams. Nanotubes come in different forms: single-walled, multiwalled, straight, and spiral, with opened and closed ends. SWCNTs have a lot of unique properties. First, it has a high mechanical strength, which is a hundred times more strength than steel and provides the possibility to use it as probes in scanning tunneling microscopy. Nanotubes are extremely durable material like tensile and bending. Moreover, under the influence of mechanical stress exceeding the critical, nanotubes do not ‘rush’ and are tunable [1]. The nanotubes can be obtained simultaneously as a rugged and elastic tissue. The electrical properties of nanotubes are determined by their chirality [2,3]. Depending on chirality, single-walled tube can exhibit properties as graphite - semimetal [4,5]. Due to the small size of carbon nanotubes, the measurement of their electrical resistivity by four-pin way was conducted as early as in 1996. The resistivity of nanotubes can vary in considerable range. The minimal resistivity of nanotubes is much lower than that for graphite. Most of the nanotubes have metallic conductivity and properties of semiconductor with band gap from 0.1 to 0.3 eV (Figure 1). The researchers also opened another property of SWCNTs such as superconductivity [6]. Unique physical and chemical properties of nanotubes are complemented by extremely interesting biological properties. Carbon nanotubes (CNTs) have great potential for application in biology and medicine for bioimaging, targeted delivery vehicles for drugs, plasmid DNA, or small interfering RNA into cells by endocytosis and biosensors, and for mimicking extracellular matrix in tissue regeneration biotechnology. There are many efforts to employ CNTs as tool for anticancer therapy. In this field, carbon nanotubes were subject for fundamental works in cell biology. But there are a lot of unclear aspects of the interaction of nanotubes with tumor cells. Toxicity and carcinogenicity has been one of the major concerns for CNTs’ use in biomedical application. Therefore, in our study, we were looking for answers on a few questions. How do carbon nanotubes affect the survival of tumor cells in monolayer culture? Which properties of nanomaterial determine its relationship with the cells? Finally, which properties of nanotubes can be used in tissue biotechnology? To answer these questions, we chose a model of multicellular tumor spheroids.Figure 1

Bottom Line: Our results demonstrated that SWCNTs at concentrations ranging from 12.5 to 50 μg/ml did not have cytotoxic influence on tumor cells; instead, they had weak cytostatic effect.The result of this influence was in formation of more MTS in cell culture with SWCNTs compared with UDDs and control samples.Our results could be useful for the control of cell growth in three-dimensional culture. 61. 46 + w; 61.48 + c; 61.48De; 87.15-v; 87.64-t.

View Article: PubMed Central - PubMed

Affiliation: Department for Biotechnical Problems of Diagnostic, Institute for Problems of Cryobiology and Cryomedicine of NAS Ukraine, 42/1 Nauky str., 03028 Kiev, Ukraine.

ABSTRACT

Abstract: This paper describes the impact of different concentrations of single-walled carbon nanotubes (SWCNTs) on cell viability of breast adenocarcinoma, MCF-7 line, and formation of multicellular tumor spheroids (MTS). Chemical composition and purity of nanotubes is controlled by Fourier transform infrared spectroscopy. The strength and direction of the influence of SWCNTs on the tumor cell population was assessed by cell counting and measurement of the volume of multicellular tumor spheroids. Effect of SWCNTs on the formation of multicellular spheroids was compared with the results obtained by culturing tumor cells with ultra dispersed diamonds (UDDs). Our results demonstrated that SWCNTs at concentrations ranging from 12.5 to 50 μg/ml did not have cytotoxic influence on tumor cells; instead, they had weak cytostatic effect. The increasing of SWCNTs concentration to 100 to 200 μg/ml stimulated proliferation of tumor cells, especially in suspension fractions. The result of this influence was in formation of more MTS in cell culture with SWCNTs compared with UDDs and control samples. In result, the median volume of MTS after cultivation with SWCNTs at 100 to 200 μg/ml concentrations is 3 to 5 times greater than that in samples which were incubated with the UDDs and is 2.5 times greater than that in control cultures. So, if SWCNTs reduced cell adhesion to substrate and stimulated formation of tumor cell aggregates volume near 7 · 10(-3) mm(3), at the same time, UDDs reduced adhesion and cohesive ability of cells and stimulated generation of cell spheroids volume no more than 4 · 10(-3) mm(3). Our results could be useful for the control of cell growth in three-dimensional culture.

Pacs: 61. 46 + w; 61.48 + c; 61.48De; 87.15-v; 87.64-t.

No MeSH data available.


Related in: MedlinePlus