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Protamine nanoparticles for improving shRNA-mediated anti-cancer effects.

Liu M, Feng B, Shi Y, Su C, Song H, Cheng W, Zhao L - Nanoscale Res Lett (2015)

Bottom Line: Protamine nanoparticles were designed by encapsulating small hairpin RNA (shRNA)-expressing plasmid DNA targeting the Bcl-2 gene (shBcl-2) to silence apoptosis-related Bcl-2 protein for improving the transfection efficiency and cytotoxicity in cancer therapy.Our findings demonstrated that the obtained protamine nanoparticles possessed excellent characterizations of small particle size, homogenous distribution, positive charge, and high encapsulation efficiency of gene. shBcl-2 loaded in nanoparticles (NPs) was protected effectively from the degradation of DNase I and serum.More importantly, it significantly improved the efficiency of transfection of shRNA in vitro in A549 cells and increased its cytotoxicity and induced more cell apoptosis by silencing Bcl-2.

View Article: PubMed Central - PubMed

Affiliation: School of Pharmacy, Liaoning Medical University, Jinzhou, 121000 People's Republic of China.

ABSTRACT
Protamine nanoparticles were designed by encapsulating small hairpin RNA (shRNA)-expressing plasmid DNA targeting the Bcl-2 gene (shBcl-2) to silence apoptosis-related Bcl-2 protein for improving the transfection efficiency and cytotoxicity in cancer therapy. Our findings demonstrated that the obtained protamine nanoparticles possessed excellent characterizations of small particle size, homogenous distribution, positive charge, and high encapsulation efficiency of gene. shBcl-2 loaded in nanoparticles (NPs) was protected effectively from the degradation of DNase I and serum. More importantly, it significantly improved the efficiency of transfection of shRNA in vitro in A549 cells and increased its cytotoxicity and induced more cell apoptosis by silencing Bcl-2.

No MeSH data available.


Related in: MedlinePlus

TEM images of NPs. shBcl-2-loaded NPs (50:1) (A), shBcl-2-loaded NPs (100:1) (B), and shBcl-2-loaded NPs (200:1) (C). DLS analysis of the obtained shBcl-2-loaded NPs (D).
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Fig1: TEM images of NPs. shBcl-2-loaded NPs (50:1) (A), shBcl-2-loaded NPs (100:1) (B), and shBcl-2-loaded NPs (200:1) (C). DLS analysis of the obtained shBcl-2-loaded NPs (D).

Mentions: The morphology and size distribution of the prepared nanoparticles were determined by TEM and DLS, and the results were displayed in Table 1. It can be seen from Table 1 that with the increasing amount of protamine, the particle size was enhanced and the zeta potential also continued to rise. In term of encapsulation efficiency of shBcl-2 in NPs, about 85% of shBcl-2 was loaded in NPs with the mass ratio at 100:1 in contrast with the other two NPs. Judging by the TEM in Figure 1, it was observed that shBcl-2-loaded NPs with different mass ratios possessed an excellent characterization of suitable particle size, homogenous size distribution, and high monodispersion, indicating that shBcl-2-loaded NPs with the mass ratio of 100:1 was a potential optimized gene carrier with good characterization and higher encapsulation efficiency.Table 1


Protamine nanoparticles for improving shRNA-mediated anti-cancer effects.

Liu M, Feng B, Shi Y, Su C, Song H, Cheng W, Zhao L - Nanoscale Res Lett (2015)

TEM images of NPs. shBcl-2-loaded NPs (50:1) (A), shBcl-2-loaded NPs (100:1) (B), and shBcl-2-loaded NPs (200:1) (C). DLS analysis of the obtained shBcl-2-loaded NPs (D).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4385308&req=5

Fig1: TEM images of NPs. shBcl-2-loaded NPs (50:1) (A), shBcl-2-loaded NPs (100:1) (B), and shBcl-2-loaded NPs (200:1) (C). DLS analysis of the obtained shBcl-2-loaded NPs (D).
Mentions: The morphology and size distribution of the prepared nanoparticles were determined by TEM and DLS, and the results were displayed in Table 1. It can be seen from Table 1 that with the increasing amount of protamine, the particle size was enhanced and the zeta potential also continued to rise. In term of encapsulation efficiency of shBcl-2 in NPs, about 85% of shBcl-2 was loaded in NPs with the mass ratio at 100:1 in contrast with the other two NPs. Judging by the TEM in Figure 1, it was observed that shBcl-2-loaded NPs with different mass ratios possessed an excellent characterization of suitable particle size, homogenous size distribution, and high monodispersion, indicating that shBcl-2-loaded NPs with the mass ratio of 100:1 was a potential optimized gene carrier with good characterization and higher encapsulation efficiency.Table 1

Bottom Line: Protamine nanoparticles were designed by encapsulating small hairpin RNA (shRNA)-expressing plasmid DNA targeting the Bcl-2 gene (shBcl-2) to silence apoptosis-related Bcl-2 protein for improving the transfection efficiency and cytotoxicity in cancer therapy.Our findings demonstrated that the obtained protamine nanoparticles possessed excellent characterizations of small particle size, homogenous distribution, positive charge, and high encapsulation efficiency of gene. shBcl-2 loaded in nanoparticles (NPs) was protected effectively from the degradation of DNase I and serum.More importantly, it significantly improved the efficiency of transfection of shRNA in vitro in A549 cells and increased its cytotoxicity and induced more cell apoptosis by silencing Bcl-2.

View Article: PubMed Central - PubMed

Affiliation: School of Pharmacy, Liaoning Medical University, Jinzhou, 121000 People's Republic of China.

ABSTRACT
Protamine nanoparticles were designed by encapsulating small hairpin RNA (shRNA)-expressing plasmid DNA targeting the Bcl-2 gene (shBcl-2) to silence apoptosis-related Bcl-2 protein for improving the transfection efficiency and cytotoxicity in cancer therapy. Our findings demonstrated that the obtained protamine nanoparticles possessed excellent characterizations of small particle size, homogenous distribution, positive charge, and high encapsulation efficiency of gene. shBcl-2 loaded in nanoparticles (NPs) was protected effectively from the degradation of DNase I and serum. More importantly, it significantly improved the efficiency of transfection of shRNA in vitro in A549 cells and increased its cytotoxicity and induced more cell apoptosis by silencing Bcl-2.

No MeSH data available.


Related in: MedlinePlus