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Biosynthesis of gold nanoparticles assisted by the intracellular protein extract of Pycnoporus sanguineus and its catalysis in degradation of 4-nitroaniline.

Shi C, Zhu N, Cao Y, Wu P - Nanoscale Res Lett (2015)

Bottom Line: Biological synthetic routes are considered to be environmentally benign and cost-effective.The catalysis results showed that 0.019 mg of AuNPs with average size of 6.07 nm could catalyze the complete degradation of 12.5 μmol of 4-nitroaniline within 6 min and the degradation rate increased drastically with the addition of AuNPs.All the results suggested that the IPE of P. sanguineus could be potentially applied for the eco-friendly synthesis of AuNPs.

View Article: PubMed Central - PubMed

Affiliation: School of Environment and Energy, South China University of Technology, Guangzhou, 510006 China.

ABSTRACT
The development of green procedure for the synthesis of gold nanoparticles (AuNPs) has gained great interest in the field of nanotechnology. Biological synthetic routes are considered to be environmentally benign and cost-effective. In the present study, the feasibility of AuNPs' synthesis via intracellular protein extract (IPE) of Pycnoporus sanguineus was explored. The characteristics of generated particles of formation, crystalline nature, and morphology and dimension were analyzed by UV-vis spectroscopy, X-ray diffraction (XRD) and transmission electron microscopy (TEM), respectively. UV-vis spectra exhibited strong absorption peaks in 520 to 560 nm, indicating the formation of AuNPs. XRD analysis revealed that the formed AuNPs were purely crystalline in nature. TEM observation showed that AuNPs with various shapes including spherical, pseudo-spherical, triangular, truncated triangular, pentagonal, and hexagonal, ranging from several to several hundred nanometers, were synthesized under different conditions. The average size of AuNPs decreased from 61.47 to 29.30 nm as the IPE addition increased from 10 to 80 mL. When the initial gold ion concentration changed from 0.5 to 2.0 mM, the average size rose from 25.88 to 51.99 nm. As in the case of solution pH, the average size was 84.29 nm with solution pH of 2.0, which diminished to 6.07 nm with solution pH of 12.0. Fourier transform infrared (FTIR) analysis implied that the functional groups including hydroxyl, amine, and carboxyl were involved in the reduction of gold ions and stabilization of AuNPs. The catalysis results showed that 0.019 mg of AuNPs with average size of 6.07 nm could catalyze the complete degradation of 12.5 μmol of 4-nitroaniline within 6 min and the degradation rate increased drastically with the addition of AuNPs. All the results suggested that the IPE of P. sanguineus could be potentially applied for the eco-friendly synthesis of AuNPs.

No MeSH data available.


Representative TEM images of AuNPs obtained under different conditions. (a) 40 mL IPE, 1.0 mM, pH = 2.7. (b) 80 mL IPE, 1.5 mM, pH = 2.5. (c) 80 mL IPE, 1.0 mM, pH = 4.0.
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Fig3: Representative TEM images of AuNPs obtained under different conditions. (a) 40 mL IPE, 1.0 mM, pH = 2.7. (b) 80 mL IPE, 1.5 mM, pH = 2.5. (c) 80 mL IPE, 1.0 mM, pH = 4.0.

Mentions: The morphology and size of biosynthesized AuNPs were estimated by TEM study. Figure 3 showed the representative TEM images of AuNPs obtained under different conditions, which illustrated the formation of AuNPs with various shapes including spherical, pseudo-spherical, triangular, truncated triangular, pentagonal, and hexagonal. The effects of IPE addition on the characteristics and particle size distributions of as synthesized AuNPs were depicted in Additional file 1: Figures S1 and S2. It can be observed that most spherical or pseudo-spherical AuNPs were relatively smaller compared with triangular, pentagonal, or hexagonal ones. With 10 mL IPE addition, many AuNPs with triangular or hexagonal shapes in the size of 100 to 500 nm were synthesized and the average size was measured to be 61.47 nm. As the IPE addition increased, the formed AuNPs exhibited narrower size distribution and smaller average particle size. When the IPE addition was increased to 80 mL, more than 90% of the AuNPs were smaller than 50 nm, almost 78% were in the range of 10 to 40 nm, and the average particle size decreased to 29.3 nm.Figure 3


Biosynthesis of gold nanoparticles assisted by the intracellular protein extract of Pycnoporus sanguineus and its catalysis in degradation of 4-nitroaniline.

Shi C, Zhu N, Cao Y, Wu P - Nanoscale Res Lett (2015)

Representative TEM images of AuNPs obtained under different conditions. (a) 40 mL IPE, 1.0 mM, pH = 2.7. (b) 80 mL IPE, 1.5 mM, pH = 2.5. (c) 80 mL IPE, 1.0 mM, pH = 4.0.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4385306&req=5

Fig3: Representative TEM images of AuNPs obtained under different conditions. (a) 40 mL IPE, 1.0 mM, pH = 2.7. (b) 80 mL IPE, 1.5 mM, pH = 2.5. (c) 80 mL IPE, 1.0 mM, pH = 4.0.
Mentions: The morphology and size of biosynthesized AuNPs were estimated by TEM study. Figure 3 showed the representative TEM images of AuNPs obtained under different conditions, which illustrated the formation of AuNPs with various shapes including spherical, pseudo-spherical, triangular, truncated triangular, pentagonal, and hexagonal. The effects of IPE addition on the characteristics and particle size distributions of as synthesized AuNPs were depicted in Additional file 1: Figures S1 and S2. It can be observed that most spherical or pseudo-spherical AuNPs were relatively smaller compared with triangular, pentagonal, or hexagonal ones. With 10 mL IPE addition, many AuNPs with triangular or hexagonal shapes in the size of 100 to 500 nm were synthesized and the average size was measured to be 61.47 nm. As the IPE addition increased, the formed AuNPs exhibited narrower size distribution and smaller average particle size. When the IPE addition was increased to 80 mL, more than 90% of the AuNPs were smaller than 50 nm, almost 78% were in the range of 10 to 40 nm, and the average particle size decreased to 29.3 nm.Figure 3

Bottom Line: Biological synthetic routes are considered to be environmentally benign and cost-effective.The catalysis results showed that 0.019 mg of AuNPs with average size of 6.07 nm could catalyze the complete degradation of 12.5 μmol of 4-nitroaniline within 6 min and the degradation rate increased drastically with the addition of AuNPs.All the results suggested that the IPE of P. sanguineus could be potentially applied for the eco-friendly synthesis of AuNPs.

View Article: PubMed Central - PubMed

Affiliation: School of Environment and Energy, South China University of Technology, Guangzhou, 510006 China.

ABSTRACT
The development of green procedure for the synthesis of gold nanoparticles (AuNPs) has gained great interest in the field of nanotechnology. Biological synthetic routes are considered to be environmentally benign and cost-effective. In the present study, the feasibility of AuNPs' synthesis via intracellular protein extract (IPE) of Pycnoporus sanguineus was explored. The characteristics of generated particles of formation, crystalline nature, and morphology and dimension were analyzed by UV-vis spectroscopy, X-ray diffraction (XRD) and transmission electron microscopy (TEM), respectively. UV-vis spectra exhibited strong absorption peaks in 520 to 560 nm, indicating the formation of AuNPs. XRD analysis revealed that the formed AuNPs were purely crystalline in nature. TEM observation showed that AuNPs with various shapes including spherical, pseudo-spherical, triangular, truncated triangular, pentagonal, and hexagonal, ranging from several to several hundred nanometers, were synthesized under different conditions. The average size of AuNPs decreased from 61.47 to 29.30 nm as the IPE addition increased from 10 to 80 mL. When the initial gold ion concentration changed from 0.5 to 2.0 mM, the average size rose from 25.88 to 51.99 nm. As in the case of solution pH, the average size was 84.29 nm with solution pH of 2.0, which diminished to 6.07 nm with solution pH of 12.0. Fourier transform infrared (FTIR) analysis implied that the functional groups including hydroxyl, amine, and carboxyl were involved in the reduction of gold ions and stabilization of AuNPs. The catalysis results showed that 0.019 mg of AuNPs with average size of 6.07 nm could catalyze the complete degradation of 12.5 μmol of 4-nitroaniline within 6 min and the degradation rate increased drastically with the addition of AuNPs. All the results suggested that the IPE of P. sanguineus could be potentially applied for the eco-friendly synthesis of AuNPs.

No MeSH data available.