Limits...
Biosynthesis of gold nanoparticles assisted by the intracellular protein extract of Pycnoporus sanguineus and its catalysis in degradation of 4-nitroaniline.

Shi C, Zhu N, Cao Y, Wu P - Nanoscale Res Lett (2015)

Bottom Line: Fourier transform infrared (FTIR) analysis implied that the functional groups including hydroxyl, amine, and carboxyl were involved in the reduction of gold ions and stabilization of AuNPs.The catalysis results showed that 0.019 mg of AuNPs with average size of 6.07 nm could catalyze the complete degradation of 12.5 μmol of 4-nitroaniline within 6 min and the degradation rate increased drastically with the addition of AuNPs.All the results suggested that the IPE of P. sanguineus could be potentially applied for the eco-friendly synthesis of AuNPs.

View Article: PubMed Central - PubMed

Affiliation: School of Environment and Energy, South China University of Technology, Guangzhou, 510006 China.

ABSTRACT
The development of green procedure for the synthesis of gold nanoparticles (AuNPs) has gained great interest in the field of nanotechnology. Biological synthetic routes are considered to be environmentally benign and cost-effective. In the present study, the feasibility of AuNPs' synthesis via intracellular protein extract (IPE) of Pycnoporus sanguineus was explored. The characteristics of generated particles of formation, crystalline nature, and morphology and dimension were analyzed by UV-vis spectroscopy, X-ray diffraction (XRD) and transmission electron microscopy (TEM), respectively. UV-vis spectra exhibited strong absorption peaks in 520 to 560 nm, indicating the formation of AuNPs. XRD analysis revealed that the formed AuNPs were purely crystalline in nature. TEM observation showed that AuNPs with various shapes including spherical, pseudo-spherical, triangular, truncated triangular, pentagonal, and hexagonal, ranging from several to several hundred nanometers, were synthesized under different conditions. The average size of AuNPs decreased from 61.47 to 29.30 nm as the IPE addition increased from 10 to 80 mL. When the initial gold ion concentration changed from 0.5 to 2.0 mM, the average size rose from 25.88 to 51.99 nm. As in the case of solution pH, the average size was 84.29 nm with solution pH of 2.0, which diminished to 6.07 nm with solution pH of 12.0. Fourier transform infrared (FTIR) analysis implied that the functional groups including hydroxyl, amine, and carboxyl were involved in the reduction of gold ions and stabilization of AuNPs. The catalysis results showed that 0.019 mg of AuNPs with average size of 6.07 nm could catalyze the complete degradation of 12.5 μmol of 4-nitroaniline within 6 min and the degradation rate increased drastically with the addition of AuNPs. All the results suggested that the IPE of P. sanguineus could be potentially applied for the eco-friendly synthesis of AuNPs.

No MeSH data available.


Related in: MedlinePlus

UV-vis spectra of the reaction mixtures recorded after 24 h of incubation under different conditions. (a) Effect of IPE addition. (b) Effect of initial gold ion concentration. (c) Effect of solution pH.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4385306&req=5

Fig1: UV-vis spectra of the reaction mixtures recorded after 24 h of incubation under different conditions. (a) Effect of IPE addition. (b) Effect of initial gold ion concentration. (c) Effect of solution pH.

Mentions: UV-vis spectroscopy has been widely considered to be a useful technique to ascertain the formation of AuNPs [21]. Figure 1 depicted the UV-vis spectra of the reaction mixtures obtained after 24 h of incubation under various conditions. Strong absorption peaks located in the range of 520 to 560 nm were observed, further confirming the formation of AuNPs with various morphology and dimension, which was in agreement with the previous report [23]. The effect of IPE addition was shown in Figure 1a. It can be seen that the intensity of maximum absorption increased with IPE addition, indicating higher AuNPs production, since the intensity was directly proportional to the density of AuNPs [16]. In addition, a blue shift of the maximum absorption was observed as the IPE addition increased, which might be attributed to the formation of AuNPs with smaller size, further verified by TEM study. Husseiny et al. [24] reported that the wavelength of maximum absorption tended to red shift as the particle size increased, which was in accordance with our results. Figure 1b illustrated the spectra of reaction mixtures with different initial gold ion concentrations. The intensity and wavelength of maximum absorption increased along with the increase of initial gold ion concentration, meaning higher production and larger particle size of AuNPs. No distinct regularity was detected with the effect of solution pH on the spectra as shown in Figure 1c, and the highest intensity was obtained with the solution pH of 4.0, while the reasons needed further research.Figure 1


Biosynthesis of gold nanoparticles assisted by the intracellular protein extract of Pycnoporus sanguineus and its catalysis in degradation of 4-nitroaniline.

Shi C, Zhu N, Cao Y, Wu P - Nanoscale Res Lett (2015)

UV-vis spectra of the reaction mixtures recorded after 24 h of incubation under different conditions. (a) Effect of IPE addition. (b) Effect of initial gold ion concentration. (c) Effect of solution pH.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4385306&req=5

Fig1: UV-vis spectra of the reaction mixtures recorded after 24 h of incubation under different conditions. (a) Effect of IPE addition. (b) Effect of initial gold ion concentration. (c) Effect of solution pH.
Mentions: UV-vis spectroscopy has been widely considered to be a useful technique to ascertain the formation of AuNPs [21]. Figure 1 depicted the UV-vis spectra of the reaction mixtures obtained after 24 h of incubation under various conditions. Strong absorption peaks located in the range of 520 to 560 nm were observed, further confirming the formation of AuNPs with various morphology and dimension, which was in agreement with the previous report [23]. The effect of IPE addition was shown in Figure 1a. It can be seen that the intensity of maximum absorption increased with IPE addition, indicating higher AuNPs production, since the intensity was directly proportional to the density of AuNPs [16]. In addition, a blue shift of the maximum absorption was observed as the IPE addition increased, which might be attributed to the formation of AuNPs with smaller size, further verified by TEM study. Husseiny et al. [24] reported that the wavelength of maximum absorption tended to red shift as the particle size increased, which was in accordance with our results. Figure 1b illustrated the spectra of reaction mixtures with different initial gold ion concentrations. The intensity and wavelength of maximum absorption increased along with the increase of initial gold ion concentration, meaning higher production and larger particle size of AuNPs. No distinct regularity was detected with the effect of solution pH on the spectra as shown in Figure 1c, and the highest intensity was obtained with the solution pH of 4.0, while the reasons needed further research.Figure 1

Bottom Line: Fourier transform infrared (FTIR) analysis implied that the functional groups including hydroxyl, amine, and carboxyl were involved in the reduction of gold ions and stabilization of AuNPs.The catalysis results showed that 0.019 mg of AuNPs with average size of 6.07 nm could catalyze the complete degradation of 12.5 μmol of 4-nitroaniline within 6 min and the degradation rate increased drastically with the addition of AuNPs.All the results suggested that the IPE of P. sanguineus could be potentially applied for the eco-friendly synthesis of AuNPs.

View Article: PubMed Central - PubMed

Affiliation: School of Environment and Energy, South China University of Technology, Guangzhou, 510006 China.

ABSTRACT
The development of green procedure for the synthesis of gold nanoparticles (AuNPs) has gained great interest in the field of nanotechnology. Biological synthetic routes are considered to be environmentally benign and cost-effective. In the present study, the feasibility of AuNPs' synthesis via intracellular protein extract (IPE) of Pycnoporus sanguineus was explored. The characteristics of generated particles of formation, crystalline nature, and morphology and dimension were analyzed by UV-vis spectroscopy, X-ray diffraction (XRD) and transmission electron microscopy (TEM), respectively. UV-vis spectra exhibited strong absorption peaks in 520 to 560 nm, indicating the formation of AuNPs. XRD analysis revealed that the formed AuNPs were purely crystalline in nature. TEM observation showed that AuNPs with various shapes including spherical, pseudo-spherical, triangular, truncated triangular, pentagonal, and hexagonal, ranging from several to several hundred nanometers, were synthesized under different conditions. The average size of AuNPs decreased from 61.47 to 29.30 nm as the IPE addition increased from 10 to 80 mL. When the initial gold ion concentration changed from 0.5 to 2.0 mM, the average size rose from 25.88 to 51.99 nm. As in the case of solution pH, the average size was 84.29 nm with solution pH of 2.0, which diminished to 6.07 nm with solution pH of 12.0. Fourier transform infrared (FTIR) analysis implied that the functional groups including hydroxyl, amine, and carboxyl were involved in the reduction of gold ions and stabilization of AuNPs. The catalysis results showed that 0.019 mg of AuNPs with average size of 6.07 nm could catalyze the complete degradation of 12.5 μmol of 4-nitroaniline within 6 min and the degradation rate increased drastically with the addition of AuNPs. All the results suggested that the IPE of P. sanguineus could be potentially applied for the eco-friendly synthesis of AuNPs.

No MeSH data available.


Related in: MedlinePlus