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Nanosized silver (II) pyridoxine complex to cause greater inflammatory response and less cytotoxicity to RAW264.7 macrophage cells.

Paul A, Ju H, Rangasamy S, Shim Y, Song JM - Nanoscale Res Lett (2015)

Bottom Line: Surprisingly, however, AgPyNPs caused macrophage RAW264.7 cells to secrete a larger amount of interleukin-8 (IL-8) and generate a more active inflammatory response compared to AgNPs.It activated TNF-α, NF-κB p65, and NF-κB p50 to generate a more vigorous immune protection that produces a greater amount of IL-8 compared to AgNPs.Thus, it can be used as a better wound-healing agent than AgNPs.

View Article: PubMed Central - PubMed

Affiliation: College of Pharmacy, Seoul National University, 1 Gwanak-ro, Gwanak-ku, Seoul, 151-742 Korea.

ABSTRACT
With advancements in nanotechnology, silver has been engineered into a nanometre size and has attracted great research interest for use in the treatment of wounds. Silver nanoparticles (AgNPs) have emerged as a potential alternative to conventional antibiotics because of their potential antimicrobial property. However, AgNPs also induce cytotoxicity, generate reactive oxygen species (ROS), and cause mitochondrial damage to human cells. Pyridoxine possesses antioxidant and cell proliferation activity. Therefore, in the present investigation, a nanosilver-pyridoxine complex (AgPyNP) was synthesized, and its cytotoxicity and immune response was compared with AgNPs in macrophage RAW264.7 cells. Results revealed that AgPyNPs showed less cytotoxicity compared with AgNPs by producing a smaller amount of ROS in RAW264.7 cells. Surprisingly, however, AgPyNPs caused macrophage RAW264.7 cells to secrete a larger amount of interleukin-8 (IL-8) and generate a more active inflammatory response compared to AgNPs. It activated TNF-α, NF-κB p65, and NF-κB p50 to generate a more vigorous immune protection that produces a greater amount of IL-8 compared to AgNPs. Overall findings indicate that AgPyNPs exhibited less cytotoxicity and evoked a greater immune response in macrophage RAW264.7 cells. Thus, it can be used as a better wound-healing agent than AgNPs. Graphical AbstractFigurative representation of the comparison of AgNPs and AgPyNPs in macrophage RAW264.7 cells in terms of cytotoxicity and immune response.

No MeSH data available.


Related in: MedlinePlus

AgNP- and AgPyNP-induced cytotoxicity in RAW264.7 cells was analysed using MTT assay. Cells were treated with different concentrations of AgNP and AgPyNP for 24 h. The values represent the mean of at least three independent experiments normalized to untreated controls. Error bars represent the standard deviations of three independent experiments. Student’s t-test was used to determine the statistical significance: **P < 0.01; ***P < 0.001. Presented data were combined from at least three experiments. AgNPs, silver nanoparticles; AgPyNPs, nanosilver-pyridoxine complexes.
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Fig2: AgNP- and AgPyNP-induced cytotoxicity in RAW264.7 cells was analysed using MTT assay. Cells were treated with different concentrations of AgNP and AgPyNP for 24 h. The values represent the mean of at least three independent experiments normalized to untreated controls. Error bars represent the standard deviations of three independent experiments. Student’s t-test was used to determine the statistical significance: **P < 0.01; ***P < 0.001. Presented data were combined from at least three experiments. AgNPs, silver nanoparticles; AgPyNPs, nanosilver-pyridoxine complexes.

Mentions: Cell viability assay was performed by MTT assay using different dosages of AgNPs/AgPyNPs in RAW264.7cells after 24 h of treatment. Untreated cells served as controls. The results of the MTT assay are shown in Figure 2. Both AgNPs/AgPyNPs showed their cytotoxicity at concentrations of 50 μg/mL and higher. AgNPs exhibited greater cytotoxicity compared to that of AgPyNPs. The 84.1%, 68.78%, 30.79%, and 21.56% viable cells were observed after 5, 10, 50, and 70 μg/mL AgNP treatments, respectively. Whereas, 92.96%, 76.28%, 46.47%, and 33.99% viable cells were observed after 5, 10, 50, and 70 μg/mL AgPyNP treatments, respectively, for 24 h. This result indicated that AgPyNPs was less cytotoxic compared to AgNPs.Figure 2


Nanosized silver (II) pyridoxine complex to cause greater inflammatory response and less cytotoxicity to RAW264.7 macrophage cells.

Paul A, Ju H, Rangasamy S, Shim Y, Song JM - Nanoscale Res Lett (2015)

AgNP- and AgPyNP-induced cytotoxicity in RAW264.7 cells was analysed using MTT assay. Cells were treated with different concentrations of AgNP and AgPyNP for 24 h. The values represent the mean of at least three independent experiments normalized to untreated controls. Error bars represent the standard deviations of three independent experiments. Student’s t-test was used to determine the statistical significance: **P < 0.01; ***P < 0.001. Presented data were combined from at least three experiments. AgNPs, silver nanoparticles; AgPyNPs, nanosilver-pyridoxine complexes.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4385296&req=5

Fig2: AgNP- and AgPyNP-induced cytotoxicity in RAW264.7 cells was analysed using MTT assay. Cells were treated with different concentrations of AgNP and AgPyNP for 24 h. The values represent the mean of at least three independent experiments normalized to untreated controls. Error bars represent the standard deviations of three independent experiments. Student’s t-test was used to determine the statistical significance: **P < 0.01; ***P < 0.001. Presented data were combined from at least three experiments. AgNPs, silver nanoparticles; AgPyNPs, nanosilver-pyridoxine complexes.
Mentions: Cell viability assay was performed by MTT assay using different dosages of AgNPs/AgPyNPs in RAW264.7cells after 24 h of treatment. Untreated cells served as controls. The results of the MTT assay are shown in Figure 2. Both AgNPs/AgPyNPs showed their cytotoxicity at concentrations of 50 μg/mL and higher. AgNPs exhibited greater cytotoxicity compared to that of AgPyNPs. The 84.1%, 68.78%, 30.79%, and 21.56% viable cells were observed after 5, 10, 50, and 70 μg/mL AgNP treatments, respectively. Whereas, 92.96%, 76.28%, 46.47%, and 33.99% viable cells were observed after 5, 10, 50, and 70 μg/mL AgPyNP treatments, respectively, for 24 h. This result indicated that AgPyNPs was less cytotoxic compared to AgNPs.Figure 2

Bottom Line: Surprisingly, however, AgPyNPs caused macrophage RAW264.7 cells to secrete a larger amount of interleukin-8 (IL-8) and generate a more active inflammatory response compared to AgNPs.It activated TNF-α, NF-κB p65, and NF-κB p50 to generate a more vigorous immune protection that produces a greater amount of IL-8 compared to AgNPs.Thus, it can be used as a better wound-healing agent than AgNPs.

View Article: PubMed Central - PubMed

Affiliation: College of Pharmacy, Seoul National University, 1 Gwanak-ro, Gwanak-ku, Seoul, 151-742 Korea.

ABSTRACT
With advancements in nanotechnology, silver has been engineered into a nanometre size and has attracted great research interest for use in the treatment of wounds. Silver nanoparticles (AgNPs) have emerged as a potential alternative to conventional antibiotics because of their potential antimicrobial property. However, AgNPs also induce cytotoxicity, generate reactive oxygen species (ROS), and cause mitochondrial damage to human cells. Pyridoxine possesses antioxidant and cell proliferation activity. Therefore, in the present investigation, a nanosilver-pyridoxine complex (AgPyNP) was synthesized, and its cytotoxicity and immune response was compared with AgNPs in macrophage RAW264.7 cells. Results revealed that AgPyNPs showed less cytotoxicity compared with AgNPs by producing a smaller amount of ROS in RAW264.7 cells. Surprisingly, however, AgPyNPs caused macrophage RAW264.7 cells to secrete a larger amount of interleukin-8 (IL-8) and generate a more active inflammatory response compared to AgNPs. It activated TNF-α, NF-κB p65, and NF-κB p50 to generate a more vigorous immune protection that produces a greater amount of IL-8 compared to AgNPs. Overall findings indicate that AgPyNPs exhibited less cytotoxicity and evoked a greater immune response in macrophage RAW264.7 cells. Thus, it can be used as a better wound-healing agent than AgNPs. Graphical AbstractFigurative representation of the comparison of AgNPs and AgPyNPs in macrophage RAW264.7 cells in terms of cytotoxicity and immune response.

No MeSH data available.


Related in: MedlinePlus