Limits...
SPECT imaging of glioma with radioiodinated CLINDE: evidence from a mouse GL26 glioma model.

Tsartsalis S, Dumas N, Tournier BB, Pham T, Moulin-Sallanon M, Grégoire MC, Charnay Y, Millet P - EJNMMI Res (2015)

Bottom Line: SPECT images were compared to results of autoradiography, fluorescence microscopy, in situ nucleic acid hybridization, histology, and immunohistochemistry.Tracer uptake by the tumor is indeed 3.26 ± 0.32 times higher than that of the contralateral striatum, and 78% of the activity is displaceable by unlabeled CLINDE.Finally, TSPO is abundantly expressed by the GL26 cells.

View Article: PubMed Central - PubMed

Affiliation: Vulnerability Biomarkers Unit, Division of General Psychiatry, Department of Mental Health and Psychiatry, University Hospitals of Geneva, Chemin du Petit-Bel-Air 2, CH1225 Geneva, Chêne-Bourg Switzerland ; Department of Psychiatry, University of Geneva, 1 rue Michel-Servet, CH1211 Geneva 4, Switzerland.

ABSTRACT

Background: Recent research has demonstrated the potential of 18-kDa translocator protein (TSPO) to serve as a target for nuclear imaging of gliomas. The aim of this study was to evaluate SPECT imaging of GL26 mouse glioma using radioiodinated CLINDE, a TSPO-specific tracer.

Methods: GL26 cells, previously transfected with an enhanced green fluorescent protein (EGFP)-expressing lentivirus, were stereotactically implanted in the striatum of C57/Bl6 mice. At 4 weeks post-injection, dynamic SPECT scans with [(123)I]CLINDE were performed. A displacement study assessed specificity of tracer binding. SPECT images were compared to results of autoradiography, fluorescence microscopy, in situ nucleic acid hybridization, histology, and immunohistochemistry. Western blotting was performed to verify TSPO production by the tumor.

Results: Specific uptake of tracer by the tumor is observed with a high signal-to-noise ratio. Tracer uptake by the tumor is indeed 3.26 ± 0.32 times higher than that of the contralateral striatum, and 78% of the activity is displaceable by unlabeled CLINDE. Finally, TSPO is abundantly expressed by the GL26 cells.

Conclusions: The present study demonstrates the feasibility of [(123)I]CLINDE SPECT in translational studies and underlines its potential for clinical glioma SPECT imaging.

No MeSH data available.


Related in: MedlinePlus

Fluorescence microscopy andin situmRNA hybridization. (A) Coronal brain section of GL-26 tumor-bearing mouse visualized after Nissl staining. (B) A coronal brain section of another mouse visualized under fluorescence microscopy depicting the EGFP-expressing GL26 cells. (C) Result of in situ TSPO mRNA hybridization performed on an adjacent brain section (box depicts part of the slice in a greater magnification).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4385259&req=5

Fig3: Fluorescence microscopy andin situmRNA hybridization. (A) Coronal brain section of GL-26 tumor-bearing mouse visualized after Nissl staining. (B) A coronal brain section of another mouse visualized under fluorescence microscopy depicting the EGFP-expressing GL26 cells. (C) Result of in situ TSPO mRNA hybridization performed on an adjacent brain section (box depicts part of the slice in a greater magnification).

Mentions: Figure 3A presents a coronal section of a tumor-bearing mouse after Nissl staining while Figure 3B depicts brain sections from another mouse that was analyzed by means of fluorescence microscopy and (Figure 3C) in situ hybridization with specific probes that demonstrate the expression of the TSPO mRNA by the tumor.Figure 3


SPECT imaging of glioma with radioiodinated CLINDE: evidence from a mouse GL26 glioma model.

Tsartsalis S, Dumas N, Tournier BB, Pham T, Moulin-Sallanon M, Grégoire MC, Charnay Y, Millet P - EJNMMI Res (2015)

Fluorescence microscopy andin situmRNA hybridization. (A) Coronal brain section of GL-26 tumor-bearing mouse visualized after Nissl staining. (B) A coronal brain section of another mouse visualized under fluorescence microscopy depicting the EGFP-expressing GL26 cells. (C) Result of in situ TSPO mRNA hybridization performed on an adjacent brain section (box depicts part of the slice in a greater magnification).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4385259&req=5

Fig3: Fluorescence microscopy andin situmRNA hybridization. (A) Coronal brain section of GL-26 tumor-bearing mouse visualized after Nissl staining. (B) A coronal brain section of another mouse visualized under fluorescence microscopy depicting the EGFP-expressing GL26 cells. (C) Result of in situ TSPO mRNA hybridization performed on an adjacent brain section (box depicts part of the slice in a greater magnification).
Mentions: Figure 3A presents a coronal section of a tumor-bearing mouse after Nissl staining while Figure 3B depicts brain sections from another mouse that was analyzed by means of fluorescence microscopy and (Figure 3C) in situ hybridization with specific probes that demonstrate the expression of the TSPO mRNA by the tumor.Figure 3

Bottom Line: SPECT images were compared to results of autoradiography, fluorescence microscopy, in situ nucleic acid hybridization, histology, and immunohistochemistry.Tracer uptake by the tumor is indeed 3.26 ± 0.32 times higher than that of the contralateral striatum, and 78% of the activity is displaceable by unlabeled CLINDE.Finally, TSPO is abundantly expressed by the GL26 cells.

View Article: PubMed Central - PubMed

Affiliation: Vulnerability Biomarkers Unit, Division of General Psychiatry, Department of Mental Health and Psychiatry, University Hospitals of Geneva, Chemin du Petit-Bel-Air 2, CH1225 Geneva, Chêne-Bourg Switzerland ; Department of Psychiatry, University of Geneva, 1 rue Michel-Servet, CH1211 Geneva 4, Switzerland.

ABSTRACT

Background: Recent research has demonstrated the potential of 18-kDa translocator protein (TSPO) to serve as a target for nuclear imaging of gliomas. The aim of this study was to evaluate SPECT imaging of GL26 mouse glioma using radioiodinated CLINDE, a TSPO-specific tracer.

Methods: GL26 cells, previously transfected with an enhanced green fluorescent protein (EGFP)-expressing lentivirus, were stereotactically implanted in the striatum of C57/Bl6 mice. At 4 weeks post-injection, dynamic SPECT scans with [(123)I]CLINDE were performed. A displacement study assessed specificity of tracer binding. SPECT images were compared to results of autoradiography, fluorescence microscopy, in situ nucleic acid hybridization, histology, and immunohistochemistry. Western blotting was performed to verify TSPO production by the tumor.

Results: Specific uptake of tracer by the tumor is observed with a high signal-to-noise ratio. Tracer uptake by the tumor is indeed 3.26 ± 0.32 times higher than that of the contralateral striatum, and 78% of the activity is displaceable by unlabeled CLINDE. Finally, TSPO is abundantly expressed by the GL26 cells.

Conclusions: The present study demonstrates the feasibility of [(123)I]CLINDE SPECT in translational studies and underlines its potential for clinical glioma SPECT imaging.

No MeSH data available.


Related in: MedlinePlus