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Diagnostic Value of Serum Adenosine Deaminase (ADA) Level for Pulmonary Tuberculosis.

Salmanzadeh S, Tavakkol H, Bavieh K, Alavi SM - Jundishapur J Microbiol (2015)

Bottom Line: In regard to the cut off value of 26 IU/L for ADA in patients with PTB sensitivity and specificity was defined as 35% and 91%, respectively.Serum ADA activity with high specificity percentage may be a useful alternative test in restricted resource areas to rule out diagnosis of PTB.However, serum ADA activity is not a useful tool for TB diagnosis.

View Article: PubMed Central - PubMed

Affiliation: Health Research Institute ,Infectious and Tropical Disease Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Ahvaz, IR Iran.

ABSTRACT

Background: Diagnosis of tuberculosis (TB) is not always easy, thus employing methods with a short duration and acceptable sensitivity and specificity is necessary to diagnose TB.

Objectives: The aim of this study was to investigate the diagnostic value of serum adenosine deaminase (ADA) level for diagnosis of pulmonary tuberculosis.

Patients and methods: A total of 160 sex and age-matched subjects were included in this study, and were divided to four groups; forty patients with pulmonary tuberculosis (PTB) diagnosed based on the national TB program (NTP), forty patients with non-tuberculosis bacterial pneumonia, forty patients with lung cancer and forty people who were healthy in every respect. Serum adenosine deaminase activity in patients of each group was measured by the Giusti and Galanti calorimetry method using a commercial kit (Diazyme, USA). The ANOVA analysis was used to compare groups for quantitative variables.

Results: Mean serum ADA level in the PTB group was clearly higher than the mean serum ADA in the other three groups. Mean serum ADA was 26 IU/L in PTB patients, 19.48 IU/L in patients with pneumonia, 15.8 IU/L in patients with lung cancer, and 10.7 IU/L in the control group (P < 0.05). In regard to the cut off value of 26 IU/L for ADA in patients with PTB sensitivity and specificity was defined as 35% and 91%, respectively.

Conclusions: Serum ADA activity with high specificity percentage may be a useful alternative test in restricted resource areas to rule out diagnosis of PTB. However, serum ADA activity is not a useful tool for TB diagnosis.

No MeSH data available.


Related in: MedlinePlus

Sensitivity and Specificity of Serum ADA Activity in Studied Subjects
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fig18059: Sensitivity and Specificity of Serum ADA Activity in Studied Subjects

Mentions: The sensitivity and specificity of the serum ADA tests were examined by the ROC curve analysis. According to curve coordinates table, if ADA cutoff point is equal to 15.5, the sensitivity and specificity will be 77% and 70%, respectively, if ADA cutoff point is equal to 19.5, the sensitivity and specificity will be 62% and 80%, respectively, and if ADA cut off point is equal to 26, the sensitivity and specificity will be 35% and 91%, respectively. A higher cut off value, results in higher specificity yet lower sensitivity (Figure 1). In order to test the effect of age on the amount of ADA, nonparametric correlations test was used. To evaluate the effect of gender on the amount of ADA (in regards to older age in cancer patients and higher male proportion in PTB group compared to the other groups), Mann-Whitney test was performed and the results showed that there was no significant relationship between sex and ADA (P = 0.509). The results also showed that there was no significant relationship between ADA and age (P = 0.222).


Diagnostic Value of Serum Adenosine Deaminase (ADA) Level for Pulmonary Tuberculosis.

Salmanzadeh S, Tavakkol H, Bavieh K, Alavi SM - Jundishapur J Microbiol (2015)

Sensitivity and Specificity of Serum ADA Activity in Studied Subjects
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4385252&req=5

fig18059: Sensitivity and Specificity of Serum ADA Activity in Studied Subjects
Mentions: The sensitivity and specificity of the serum ADA tests were examined by the ROC curve analysis. According to curve coordinates table, if ADA cutoff point is equal to 15.5, the sensitivity and specificity will be 77% and 70%, respectively, if ADA cutoff point is equal to 19.5, the sensitivity and specificity will be 62% and 80%, respectively, and if ADA cut off point is equal to 26, the sensitivity and specificity will be 35% and 91%, respectively. A higher cut off value, results in higher specificity yet lower sensitivity (Figure 1). In order to test the effect of age on the amount of ADA, nonparametric correlations test was used. To evaluate the effect of gender on the amount of ADA (in regards to older age in cancer patients and higher male proportion in PTB group compared to the other groups), Mann-Whitney test was performed and the results showed that there was no significant relationship between sex and ADA (P = 0.509). The results also showed that there was no significant relationship between ADA and age (P = 0.222).

Bottom Line: In regard to the cut off value of 26 IU/L for ADA in patients with PTB sensitivity and specificity was defined as 35% and 91%, respectively.Serum ADA activity with high specificity percentage may be a useful alternative test in restricted resource areas to rule out diagnosis of PTB.However, serum ADA activity is not a useful tool for TB diagnosis.

View Article: PubMed Central - PubMed

Affiliation: Health Research Institute ,Infectious and Tropical Disease Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Ahvaz, IR Iran.

ABSTRACT

Background: Diagnosis of tuberculosis (TB) is not always easy, thus employing methods with a short duration and acceptable sensitivity and specificity is necessary to diagnose TB.

Objectives: The aim of this study was to investigate the diagnostic value of serum adenosine deaminase (ADA) level for diagnosis of pulmonary tuberculosis.

Patients and methods: A total of 160 sex and age-matched subjects were included in this study, and were divided to four groups; forty patients with pulmonary tuberculosis (PTB) diagnosed based on the national TB program (NTP), forty patients with non-tuberculosis bacterial pneumonia, forty patients with lung cancer and forty people who were healthy in every respect. Serum adenosine deaminase activity in patients of each group was measured by the Giusti and Galanti calorimetry method using a commercial kit (Diazyme, USA). The ANOVA analysis was used to compare groups for quantitative variables.

Results: Mean serum ADA level in the PTB group was clearly higher than the mean serum ADA in the other three groups. Mean serum ADA was 26 IU/L in PTB patients, 19.48 IU/L in patients with pneumonia, 15.8 IU/L in patients with lung cancer, and 10.7 IU/L in the control group (P < 0.05). In regard to the cut off value of 26 IU/L for ADA in patients with PTB sensitivity and specificity was defined as 35% and 91%, respectively.

Conclusions: Serum ADA activity with high specificity percentage may be a useful alternative test in restricted resource areas to rule out diagnosis of PTB. However, serum ADA activity is not a useful tool for TB diagnosis.

No MeSH data available.


Related in: MedlinePlus