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Direct patterning of gold nanoparticles using flexographic printing for biosensing applications.

Benson J, Fung CM, Lloyd JS, Deganello D, Smith NA, Teng KS - Nanoscale Res Lett (2015)

Bottom Line: The average size of the printed AuNPs is less than 60 nm.Glucose sensing tests were performed using printed carbon-AuNP electrodes functionalized with glucose oxidase (GOx).We have demonstrated the fabrication of AuNP-based biosensors using flexographic printing, which is ideal for low-cost, high-volume production of the devices.

View Article: PubMed Central - PubMed

Affiliation: Multidisciplinary Nanotechnology Centre, College of Engineering, Swansea University, Singleton Park, Swansea, SA2 8PP UK.

ABSTRACT
In this paper, we have presented the use of flexographic printing techniques in the selective patterning of gold nanoparticles (AuNPs) onto a substrate. Highly uniform coverage of AuNPs was selectively patterned on the substrate surface, which was subsequently used in the development of a glucose sensor. These AuNPs provide a biocompatible site for the attachment of enzymes and offer high sensitivity in the detection of glucose due to their large surface to volume ratio. The average size of the printed AuNPs is less than 60 nm. Glucose sensing tests were performed using printed carbon-AuNP electrodes functionalized with glucose oxidase (GOx). The results showed a high sensitivity of 5.52 μA mM(-1) cm(-2) with a detection limit of 26 μM. We have demonstrated the fabrication of AuNP-based biosensors using flexographic printing, which is ideal for low-cost, high-volume production of the devices.

No MeSH data available.


Calibration curve for the polyimide-carbon-AuNP-GOx electrode responding to glucose. Plot of steady state current against concentration of glucose taken from the chronoamperometric data for a polyimide-carbon-AuNP-GOx electrode. The graph shows the linear response of the electrode for glucose additions up to 1.5 mM.
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Fig9: Calibration curve for the polyimide-carbon-AuNP-GOx electrode responding to glucose. Plot of steady state current against concentration of glucose taken from the chronoamperometric data for a polyimide-carbon-AuNP-GOx electrode. The graph shows the linear response of the electrode for glucose additions up to 1.5 mM.

Mentions: Figure 9 is the calibration curve for the glucose sensor. The graph shows a very good linear response to glucose additions, resulting in an R2 value of 0.9979. The linear range of the sensor was from 0.01 to 1.5 mM. After this point, the device began to saturate which was indicated by a plateau beginning to form with additions after 1.5 mM. The sensor displayed a high sensitivity of 5.52 μA mM−1 cm−2 with a detection limit of 26 μM. The limit of detection was calculated using the following formula:\documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$ \mathrm{L}\mathrm{o}\mathrm{D} = 3RS{D}_{\mathrm{B}}/{I}_{\mathrm{mM}} $$\end{document}LoD=3RSDB/ImM(RSDB is the relative standard deviation of blank signal, and ImM is the current per millimole).Figure 9


Direct patterning of gold nanoparticles using flexographic printing for biosensing applications.

Benson J, Fung CM, Lloyd JS, Deganello D, Smith NA, Teng KS - Nanoscale Res Lett (2015)

Calibration curve for the polyimide-carbon-AuNP-GOx electrode responding to glucose. Plot of steady state current against concentration of glucose taken from the chronoamperometric data for a polyimide-carbon-AuNP-GOx electrode. The graph shows the linear response of the electrode for glucose additions up to 1.5 mM.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4385249&req=5

Fig9: Calibration curve for the polyimide-carbon-AuNP-GOx electrode responding to glucose. Plot of steady state current against concentration of glucose taken from the chronoamperometric data for a polyimide-carbon-AuNP-GOx electrode. The graph shows the linear response of the electrode for glucose additions up to 1.5 mM.
Mentions: Figure 9 is the calibration curve for the glucose sensor. The graph shows a very good linear response to glucose additions, resulting in an R2 value of 0.9979. The linear range of the sensor was from 0.01 to 1.5 mM. After this point, the device began to saturate which was indicated by a plateau beginning to form with additions after 1.5 mM. The sensor displayed a high sensitivity of 5.52 μA mM−1 cm−2 with a detection limit of 26 μM. The limit of detection was calculated using the following formula:\documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$ \mathrm{L}\mathrm{o}\mathrm{D} = 3RS{D}_{\mathrm{B}}/{I}_{\mathrm{mM}} $$\end{document}LoD=3RSDB/ImM(RSDB is the relative standard deviation of blank signal, and ImM is the current per millimole).Figure 9

Bottom Line: The average size of the printed AuNPs is less than 60 nm.Glucose sensing tests were performed using printed carbon-AuNP electrodes functionalized with glucose oxidase (GOx).We have demonstrated the fabrication of AuNP-based biosensors using flexographic printing, which is ideal for low-cost, high-volume production of the devices.

View Article: PubMed Central - PubMed

Affiliation: Multidisciplinary Nanotechnology Centre, College of Engineering, Swansea University, Singleton Park, Swansea, SA2 8PP UK.

ABSTRACT
In this paper, we have presented the use of flexographic printing techniques in the selective patterning of gold nanoparticles (AuNPs) onto a substrate. Highly uniform coverage of AuNPs was selectively patterned on the substrate surface, which was subsequently used in the development of a glucose sensor. These AuNPs provide a biocompatible site for the attachment of enzymes and offer high sensitivity in the detection of glucose due to their large surface to volume ratio. The average size of the printed AuNPs is less than 60 nm. Glucose sensing tests were performed using printed carbon-AuNP electrodes functionalized with glucose oxidase (GOx). The results showed a high sensitivity of 5.52 μA mM(-1) cm(-2) with a detection limit of 26 μM. We have demonstrated the fabrication of AuNP-based biosensors using flexographic printing, which is ideal for low-cost, high-volume production of the devices.

No MeSH data available.