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A new strategy for TiO2 whiskers mediated multi-mode cancer treatment.

Xu P, Wang R, Ouyang J, Chen B - Nanoscale Res Lett (2015)

Bottom Line: Traditional Chinese medicine (TCM) which functions as chemotherapeutic or adjuvantly chemotherapeutic agents has been drawing a great many eyeballs for its easy obtain and significant antitumor effects accompanied with less toxic and side effects.These results identify TiO2 Ws of good biocompatibility and photocatalytic activity.These results reveal that such modality combinations put forward a promising proposal in cancer therapy.

View Article: PubMed Central - PubMed

Affiliation: Department of Hematology, The Affiliated Drum Tower Hospital of Nanjing University Medical School, Nanjing, 210008 People's Republic of China.

ABSTRACT
Traditional Chinese medicine (TCM) which functions as chemotherapeutic or adjuvantly chemotherapeutic agents has been drawing a great many eyeballs for its easy obtain and significant antitumor effects accompanied with less toxic and side effects. PDT (photodynamic therapy) utilizes the fact that certain compounds coined as photosensitizers, when exposed to light of a specific wavelength, are capable of generating cytotoxic reactive oxygen species (ROS) such as hydroxyl radical, hydrogen peroxide, and superoxide to kill cancer cells. Combinations of cancer therapeutic modalities are studied to improve the efficacy of treatment. This study aimed to explore a new strategy of coupling of titanium dioxide whiskers (TiO2 Ws) with the anticancer drug gambogic acid (GA) in photodynamic therapy. The nanocomposites were coined as GA-TiO2. The combination of TiO2 Ws with GA induced a remarkable enhancement in antitumor activity estimated by MTT assay, nuclear DAPI staining, and flow cytometry. Furthermore, the possible signaling pathway was explored by reverse transcription polymerase chain reaction (RT-PCR) and Western blot assay. These results identify TiO2 Ws of good biocompatibility and photocatalytic activity. In human leukemia cells (K562 cells), TiO2 Ws could obviously increase the intracellular concentration of GA and enhance its potential antitumor efficiency, suggesting that TiO2 Ws could act as an efficient drug delivery carrier targeting GA to carcinoma cells. Moreover, photodynamic GA-TiO2 nanocomposites could induce an evident reinforcement in antitumor activity with UV illumination. These results reveal that such modality combinations put forward a promising proposal in cancer therapy.

No MeSH data available.


Related in: MedlinePlus

In vitroGA release behaviors at pH 7.4 and 6.0 respectively.
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Fig4: In vitroGA release behaviors at pH 7.4 and 6.0 respectively.

Mentions: The EE and LE of GA-TiO2 nanocomposites were assessed and calculated as 74.53% ± 5.43% and 15.11% ± 2.36%, respectively, showing a promising option of TiO2 Ws-loaded GA to act as an anticancer drug delivery carrier. It could be seen from Figure 4 that the release of drug molecules was dependent on the pH of the medium, as well as the releasing time. Within 24 h, the drug release ratio was 36% at pH 7.4, which was slow and sustained, whereas at pH 6.0, the GA release rate was much faster, with approximately 87.5%. As mentioned above, the clinical application of GA has been limited because of its serious side effects. The result suggests that the side effects to the normal tissues could be greatly reduced due to the prolonged GA retention time in blood circulation, which was down to the pH-triggered release behavior, namely in the environment of pH 7.4. In the normal physiological conditions, most GA is hypothesized to remain in the carrier for a considerable time. Once the GA-TiO2 nanocomposites are taken up by cancer cells via endocytotic process, a faster release may occur at lower local pH, i.e., surrounding the tumor site or inside the endosome and lysosome of tumor cells, causing a tremendous development in cancer treatment nowadays.Figure 4


A new strategy for TiO2 whiskers mediated multi-mode cancer treatment.

Xu P, Wang R, Ouyang J, Chen B - Nanoscale Res Lett (2015)

In vitroGA release behaviors at pH 7.4 and 6.0 respectively.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4385221&req=5

Fig4: In vitroGA release behaviors at pH 7.4 and 6.0 respectively.
Mentions: The EE and LE of GA-TiO2 nanocomposites were assessed and calculated as 74.53% ± 5.43% and 15.11% ± 2.36%, respectively, showing a promising option of TiO2 Ws-loaded GA to act as an anticancer drug delivery carrier. It could be seen from Figure 4 that the release of drug molecules was dependent on the pH of the medium, as well as the releasing time. Within 24 h, the drug release ratio was 36% at pH 7.4, which was slow and sustained, whereas at pH 6.0, the GA release rate was much faster, with approximately 87.5%. As mentioned above, the clinical application of GA has been limited because of its serious side effects. The result suggests that the side effects to the normal tissues could be greatly reduced due to the prolonged GA retention time in blood circulation, which was down to the pH-triggered release behavior, namely in the environment of pH 7.4. In the normal physiological conditions, most GA is hypothesized to remain in the carrier for a considerable time. Once the GA-TiO2 nanocomposites are taken up by cancer cells via endocytotic process, a faster release may occur at lower local pH, i.e., surrounding the tumor site or inside the endosome and lysosome of tumor cells, causing a tremendous development in cancer treatment nowadays.Figure 4

Bottom Line: Traditional Chinese medicine (TCM) which functions as chemotherapeutic or adjuvantly chemotherapeutic agents has been drawing a great many eyeballs for its easy obtain and significant antitumor effects accompanied with less toxic and side effects.These results identify TiO2 Ws of good biocompatibility and photocatalytic activity.These results reveal that such modality combinations put forward a promising proposal in cancer therapy.

View Article: PubMed Central - PubMed

Affiliation: Department of Hematology, The Affiliated Drum Tower Hospital of Nanjing University Medical School, Nanjing, 210008 People's Republic of China.

ABSTRACT
Traditional Chinese medicine (TCM) which functions as chemotherapeutic or adjuvantly chemotherapeutic agents has been drawing a great many eyeballs for its easy obtain and significant antitumor effects accompanied with less toxic and side effects. PDT (photodynamic therapy) utilizes the fact that certain compounds coined as photosensitizers, when exposed to light of a specific wavelength, are capable of generating cytotoxic reactive oxygen species (ROS) such as hydroxyl radical, hydrogen peroxide, and superoxide to kill cancer cells. Combinations of cancer therapeutic modalities are studied to improve the efficacy of treatment. This study aimed to explore a new strategy of coupling of titanium dioxide whiskers (TiO2 Ws) with the anticancer drug gambogic acid (GA) in photodynamic therapy. The nanocomposites were coined as GA-TiO2. The combination of TiO2 Ws with GA induced a remarkable enhancement in antitumor activity estimated by MTT assay, nuclear DAPI staining, and flow cytometry. Furthermore, the possible signaling pathway was explored by reverse transcription polymerase chain reaction (RT-PCR) and Western blot assay. These results identify TiO2 Ws of good biocompatibility and photocatalytic activity. In human leukemia cells (K562 cells), TiO2 Ws could obviously increase the intracellular concentration of GA and enhance its potential antitumor efficiency, suggesting that TiO2 Ws could act as an efficient drug delivery carrier targeting GA to carcinoma cells. Moreover, photodynamic GA-TiO2 nanocomposites could induce an evident reinforcement in antitumor activity with UV illumination. These results reveal that such modality combinations put forward a promising proposal in cancer therapy.

No MeSH data available.


Related in: MedlinePlus