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Screening, identification and optimization of a yeast strain, Candida palmioleophila JKS4, capable of azo dye decolorization.

Jafari N, Kasra-Kermanshahi R, Soudi MR - Iran J Microbiol (2013)

Bottom Line: With elongated incubation period, complete decolorization was observed in presence of all dyes.From the physiological properties and phylogenetic analysis based on the 26S rDNA sequences, strain JKS4 was classified into Candida palmioleophila.Because of high decolorizing activity against various azo dyes commonly used in the textile industries, it is proposed that the isolated yeast may have a practical application in the biotransformation of various dye effluents.

View Article: PubMed Central - PubMed

Affiliation: Department of Biology, Faculty of Science, Alzahra University, Tehran, Iran.

ABSTRACT

Background and objectives: Synthetic dyes are recalcitrant to degradation and toxic to different organisms. Decolorization of textile wastewaters is one of the major concerns since last decades. Physical-chemical treatments are very expensive and frequently producing large amounts of toxic wastes. Biological treatments can be more convenient. In the present study, an attempt has been made for decolorization of azo dyes using microbial process.

Material and methods: Screening of microorganisms capable of azo dye decolorization was performed from activated sludge. The decolorization of various dyes (Reactive Black 5, Reactive Orange 16, Reactive Red 198, Direct Blue 71, Direct Yellow 12 and Direct Black 22) was determined by measuring the absorbance of culture supernatant at their λmax. Culture supernatants were also analyzed for UV-Vis absorption between 200-800 nm. The effect of aeration, temperature, different concentrations of glucose and NaCl was studied with an aim to determine the optimal conditions required for maximum decolorization.

Results: The yeast (strain JKS4) which had high ability to decolorize different azo dyes was isolated. Under aerobic condition, the yeast strain showed 85.7% of decolorization at 200 mg/l Reactive Black 5 (as a model azo dye), 1% (w/v) glucose concentration and 35°C after 24 h. All the examined dyes were extensively decolorized (53.35-97.9%) after 24 h. With elongated incubation period, complete decolorization was observed in presence of all dyes. From the physiological properties and phylogenetic analysis based on the 26S rDNA sequences, strain JKS4 was classified into Candida palmioleophila.

Conclusions: Because of high decolorizing activity against various azo dyes commonly used in the textile industries, it is proposed that the isolated yeast may have a practical application in the biotransformation of various dye effluents.

No MeSH data available.


Related in: MedlinePlus

The phylogenetic tree was constructed using neighbor-joining analysis of the 26S rDNA D1/D2 domain. The ascomycetous yeast Schizosaccharomycespombe was used as an out group. Gene bank accession numbers of references sequences are given in parenthesis.
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Figure 2: The phylogenetic tree was constructed using neighbor-joining analysis of the 26S rDNA D1/D2 domain. The ascomycetous yeast Schizosaccharomycespombe was used as an out group. Gene bank accession numbers of references sequences are given in parenthesis.

Mentions: An azo dye degrading yeast (strain JKS4) which decolorized different azo dyes was isolated from activated sludge. From the phylogenetic analysis based on the 26S rDNA sequences, strain JKS4 showed 99% similarity to Candida palmioleophila. Fig. 2 shows the phylogenetic relationship between different members of the genus Candida and isolated yeast JKS4. The 26S rDNA partial sequence of the isolated yeast JKS4 was deposited in the GenBank database with the accession number JQ650231. Some of physiological properties of the strain JKS4 were showed in Table 1. The isolate yeast exhibited decolorization ability of 82.45% at 32°C after 24 h incubation under aerobic condition. With extention of incubation time, complete decolorization was observed. Lower decolorization of 10.5% was obtained after 24 h incubation under static condition.


Screening, identification and optimization of a yeast strain, Candida palmioleophila JKS4, capable of azo dye decolorization.

Jafari N, Kasra-Kermanshahi R, Soudi MR - Iran J Microbiol (2013)

The phylogenetic tree was constructed using neighbor-joining analysis of the 26S rDNA D1/D2 domain. The ascomycetous yeast Schizosaccharomycespombe was used as an out group. Gene bank accession numbers of references sequences are given in parenthesis.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4385174&req=5

Figure 2: The phylogenetic tree was constructed using neighbor-joining analysis of the 26S rDNA D1/D2 domain. The ascomycetous yeast Schizosaccharomycespombe was used as an out group. Gene bank accession numbers of references sequences are given in parenthesis.
Mentions: An azo dye degrading yeast (strain JKS4) which decolorized different azo dyes was isolated from activated sludge. From the phylogenetic analysis based on the 26S rDNA sequences, strain JKS4 showed 99% similarity to Candida palmioleophila. Fig. 2 shows the phylogenetic relationship between different members of the genus Candida and isolated yeast JKS4. The 26S rDNA partial sequence of the isolated yeast JKS4 was deposited in the GenBank database with the accession number JQ650231. Some of physiological properties of the strain JKS4 were showed in Table 1. The isolate yeast exhibited decolorization ability of 82.45% at 32°C after 24 h incubation under aerobic condition. With extention of incubation time, complete decolorization was observed. Lower decolorization of 10.5% was obtained after 24 h incubation under static condition.

Bottom Line: With elongated incubation period, complete decolorization was observed in presence of all dyes.From the physiological properties and phylogenetic analysis based on the 26S rDNA sequences, strain JKS4 was classified into Candida palmioleophila.Because of high decolorizing activity against various azo dyes commonly used in the textile industries, it is proposed that the isolated yeast may have a practical application in the biotransformation of various dye effluents.

View Article: PubMed Central - PubMed

Affiliation: Department of Biology, Faculty of Science, Alzahra University, Tehran, Iran.

ABSTRACT

Background and objectives: Synthetic dyes are recalcitrant to degradation and toxic to different organisms. Decolorization of textile wastewaters is one of the major concerns since last decades. Physical-chemical treatments are very expensive and frequently producing large amounts of toxic wastes. Biological treatments can be more convenient. In the present study, an attempt has been made for decolorization of azo dyes using microbial process.

Material and methods: Screening of microorganisms capable of azo dye decolorization was performed from activated sludge. The decolorization of various dyes (Reactive Black 5, Reactive Orange 16, Reactive Red 198, Direct Blue 71, Direct Yellow 12 and Direct Black 22) was determined by measuring the absorbance of culture supernatant at their λmax. Culture supernatants were also analyzed for UV-Vis absorption between 200-800 nm. The effect of aeration, temperature, different concentrations of glucose and NaCl was studied with an aim to determine the optimal conditions required for maximum decolorization.

Results: The yeast (strain JKS4) which had high ability to decolorize different azo dyes was isolated. Under aerobic condition, the yeast strain showed 85.7% of decolorization at 200 mg/l Reactive Black 5 (as a model azo dye), 1% (w/v) glucose concentration and 35°C after 24 h. All the examined dyes were extensively decolorized (53.35-97.9%) after 24 h. With elongated incubation period, complete decolorization was observed in presence of all dyes. From the physiological properties and phylogenetic analysis based on the 26S rDNA sequences, strain JKS4 was classified into Candida palmioleophila.

Conclusions: Because of high decolorizing activity against various azo dyes commonly used in the textile industries, it is proposed that the isolated yeast may have a practical application in the biotransformation of various dye effluents.

No MeSH data available.


Related in: MedlinePlus