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Inhibition of aflatoxin biosynthesis in Aspergillus flavus by phenolic compounds extracted of Piper betle L.

Yazdani D, Mior Ahmad ZA, Yee How T, Jaganath IB, Shahnazi S - Iran J Microbiol (2013)

Bottom Line: The results, evaluated by measuring the mycelial growth and quantification of aflatoxin B1(AFLB1) production in broth medium revealed that chloroform soluble compounds extract from P. betle dried leaves was able to block the aflatoxin biosynthesis pathway at concentration of 500μg/ml without a significant effect on mycelium growth.The results showed that the certain phenolic compounds are able to decline the aflatoxin production in A. flavus with no significant effect on the fungus mycelia growth.The result also suggested P. betle could be used as potential antitoxin product.

View Article: PubMed Central - PubMed

Affiliation: Pharmacognosy & Pharmaceutic Department of Medicinal Plants Research Center, Institute of Medicinal Plants-ACECR, Karaj, Iran ; Department of Plant Protection, Faculty of Agriculture, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.

ABSTRACT

Background and objectives: Food contamination by aflatoxins is an important food safety concern for agricultural products. In order to identify and develop novel antifungal agents, several plant extracts and isolated compounds have been evaluated for their bioactivities. Anti-infectious activity of Piper betle used in traditional medicine of Malaysia has been reported previously.

Materials and methods: Crude methanol extract from P. betel powdered leaves was partitioned between chloroform and water. The fractions were tested against A. flavus UPMC 89, a strong aflatoxin producing strain. Inhibition of mycelial growth and aflatoxin biosynthesis were tested by disk diffusion and macrodillution techniques, respectively. The presence of aflatoxin was determined by thin-layer chromatography (TLC) and fluorescence spectroscopy techniques using AFB1 standard. The chloroform soluble compounds were identified using HPLC-Tandem mass spectrometry technique.

Results: The results, evaluated by measuring the mycelial growth and quantification of aflatoxin B1(AFLB1) production in broth medium revealed that chloroform soluble compounds extract from P. betle dried leaves was able to block the aflatoxin biosynthesis pathway at concentration of 500μg/ml without a significant effect on mycelium growth. In analyzing of this effective fractions using HPLC-MS(2) with ESI ionization technique, 11 phenolic compounds were identified.

Conclusion: The results showed that the certain phenolic compounds are able to decline the aflatoxin production in A. flavus with no significant effect on the fungus mycelia growth. The result also suggested P. betle could be used as potential antitoxin product.

No MeSH data available.


Related in: MedlinePlus

Effect of P. betle chloroform fraction on A. flavus mycelial weight and aflatoxin production.
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Figure 1: Effect of P. betle chloroform fraction on A. flavus mycelial weight and aflatoxin production.

Mentions: This fraction at 500μg/ml caused a reduction (91%) in mycelial growth and completely inhibited toxin biosynthesis by A. flavus in present experiment. Concentration of 100 μg/ml caused a significant ( p ≥ 0.05) reduction in toxin biosynthesis (69.4%) without any significant effect on mycelial growth (9%) (Fig. 1). Many researchers have shown that aflatoxin production is not related to fungal mycelial growth (24).


Inhibition of aflatoxin biosynthesis in Aspergillus flavus by phenolic compounds extracted of Piper betle L.

Yazdani D, Mior Ahmad ZA, Yee How T, Jaganath IB, Shahnazi S - Iran J Microbiol (2013)

Effect of P. betle chloroform fraction on A. flavus mycelial weight and aflatoxin production.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4385173&req=5

Figure 1: Effect of P. betle chloroform fraction on A. flavus mycelial weight and aflatoxin production.
Mentions: This fraction at 500μg/ml caused a reduction (91%) in mycelial growth and completely inhibited toxin biosynthesis by A. flavus in present experiment. Concentration of 100 μg/ml caused a significant ( p ≥ 0.05) reduction in toxin biosynthesis (69.4%) without any significant effect on mycelial growth (9%) (Fig. 1). Many researchers have shown that aflatoxin production is not related to fungal mycelial growth (24).

Bottom Line: The results, evaluated by measuring the mycelial growth and quantification of aflatoxin B1(AFLB1) production in broth medium revealed that chloroform soluble compounds extract from P. betle dried leaves was able to block the aflatoxin biosynthesis pathway at concentration of 500μg/ml without a significant effect on mycelium growth.The results showed that the certain phenolic compounds are able to decline the aflatoxin production in A. flavus with no significant effect on the fungus mycelia growth.The result also suggested P. betle could be used as potential antitoxin product.

View Article: PubMed Central - PubMed

Affiliation: Pharmacognosy & Pharmaceutic Department of Medicinal Plants Research Center, Institute of Medicinal Plants-ACECR, Karaj, Iran ; Department of Plant Protection, Faculty of Agriculture, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.

ABSTRACT

Background and objectives: Food contamination by aflatoxins is an important food safety concern for agricultural products. In order to identify and develop novel antifungal agents, several plant extracts and isolated compounds have been evaluated for their bioactivities. Anti-infectious activity of Piper betle used in traditional medicine of Malaysia has been reported previously.

Materials and methods: Crude methanol extract from P. betel powdered leaves was partitioned between chloroform and water. The fractions were tested against A. flavus UPMC 89, a strong aflatoxin producing strain. Inhibition of mycelial growth and aflatoxin biosynthesis were tested by disk diffusion and macrodillution techniques, respectively. The presence of aflatoxin was determined by thin-layer chromatography (TLC) and fluorescence spectroscopy techniques using AFB1 standard. The chloroform soluble compounds were identified using HPLC-Tandem mass spectrometry technique.

Results: The results, evaluated by measuring the mycelial growth and quantification of aflatoxin B1(AFLB1) production in broth medium revealed that chloroform soluble compounds extract from P. betle dried leaves was able to block the aflatoxin biosynthesis pathway at concentration of 500μg/ml without a significant effect on mycelium growth. In analyzing of this effective fractions using HPLC-MS(2) with ESI ionization technique, 11 phenolic compounds were identified.

Conclusion: The results showed that the certain phenolic compounds are able to decline the aflatoxin production in A. flavus with no significant effect on the fungus mycelia growth. The result also suggested P. betle could be used as potential antitoxin product.

No MeSH data available.


Related in: MedlinePlus