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The effect of ajowan (Carum copticum L.) essential oils on eukaryotic ruminal microorganisms of Mehraban sheep.

Talebzadeh R, Alipour D - Iran J Microbiol (2013)

Bottom Line: Also, an inhibitory effect was seen in concentration of ciliated protozoa and some genus were completely disappeared at the doses of 300 ppm and higher.The doses used in this study reduced the fibrolytic activity of the studied rumen microorganisms which is undesirable in practical animal nutrition.Further research is needed to assess the effects of AEO at lower doses on these parameters and also proteolysis and methanogenesis.

View Article: PubMed Central - PubMed

Affiliation: Department of Animal Science, Faculty of Agriculture, Bu-Ali Sina University, Hamedan, Iran.

ABSTRACT

Background and objectives: Essential oils may improve the utilization of nutrients by ruminal microorganisms. The aim of this study was to assess the effect of different doses of ajowan essential oils (AEO) on growth and fibrolytic activity of anaerobic fungi, and generic distribution of ciliated protozoa (in vitro).

Material and methods: Different doses of AEO (0, 150, 300, 450 and 600 ppm) were added to experimental tubes. The effect of AEO was evaluated on growth and fibrolytic enzyme activity of an anaerobic fungus (Neocalimastix spp.). Generic distribution of ciliated protozoa were evaluated in response to different doses of AEO.

Results and conclusion: The growth of fungus (Neocalimastix spp.) were inhibited and activity of fibrolytic enzymes of fungus were reduced by adding AEO. Also, an inhibitory effect was seen in concentration of ciliated protozoa and some genus were completely disappeared at the doses of 300 ppm and higher. The doses used in this study reduced the fibrolytic activity of the studied rumen microorganisms which is undesirable in practical animal nutrition. Further research is needed to assess the effects of AEO at lower doses on these parameters and also proteolysis and methanogenesis.

No MeSH data available.


The effect of different doses of AEO on gas production and filter paper digestion by isolated anaerobic fungus from Mehraban sheep.
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Figure 1: The effect of different doses of AEO on gas production and filter paper digestion by isolated anaerobic fungus from Mehraban sheep.

Mentions: Inclusion of AEO to fungal culture media completely inhibited filter paper digestion (Fig. 1). Adding AEO reduced gas production by Neocalimastix spp. after seven days of incubation from 20.7 ml to 4.3, 4.9, 4.2 and 3.8 ml for control, 150, 300, 450 and 600 ppm, respectively. The effect of AEO on extracellular avicelase activity was in a dose dependent manner, and at the doses of 600 ppm minimum activity was observed (Table 1). The activity of this enzyme was also declined in cell debris by adding AEO. The extracellular β-glucosidase activity was lower than cell associated activity and less affected by inclusion of AEO (Table 1). During this experiment five groups of ciliated protozoa were observed (i.e., Entodinium spp., Epidinium caudatum, Epidinium ecaudatum, Isotricha ssp. and Dasytricha spp.). The results showed that AEO had a strong antiprotozoal activity (Table 2). With increasing the doses of AEO, the concentrations of Entodinium spp., Epidinium caudatum and Epidinium ecaudatum were singnificantly decreased. At the doses higher than 150 ppm, Isotricha ssp. and Dasytricha spp. were completely disappeared.


The effect of ajowan (Carum copticum L.) essential oils on eukaryotic ruminal microorganisms of Mehraban sheep.

Talebzadeh R, Alipour D - Iran J Microbiol (2013)

The effect of different doses of AEO on gas production and filter paper digestion by isolated anaerobic fungus from Mehraban sheep.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4385171&req=5

Figure 1: The effect of different doses of AEO on gas production and filter paper digestion by isolated anaerobic fungus from Mehraban sheep.
Mentions: Inclusion of AEO to fungal culture media completely inhibited filter paper digestion (Fig. 1). Adding AEO reduced gas production by Neocalimastix spp. after seven days of incubation from 20.7 ml to 4.3, 4.9, 4.2 and 3.8 ml for control, 150, 300, 450 and 600 ppm, respectively. The effect of AEO on extracellular avicelase activity was in a dose dependent manner, and at the doses of 600 ppm minimum activity was observed (Table 1). The activity of this enzyme was also declined in cell debris by adding AEO. The extracellular β-glucosidase activity was lower than cell associated activity and less affected by inclusion of AEO (Table 1). During this experiment five groups of ciliated protozoa were observed (i.e., Entodinium spp., Epidinium caudatum, Epidinium ecaudatum, Isotricha ssp. and Dasytricha spp.). The results showed that AEO had a strong antiprotozoal activity (Table 2). With increasing the doses of AEO, the concentrations of Entodinium spp., Epidinium caudatum and Epidinium ecaudatum were singnificantly decreased. At the doses higher than 150 ppm, Isotricha ssp. and Dasytricha spp. were completely disappeared.

Bottom Line: Also, an inhibitory effect was seen in concentration of ciliated protozoa and some genus were completely disappeared at the doses of 300 ppm and higher.The doses used in this study reduced the fibrolytic activity of the studied rumen microorganisms which is undesirable in practical animal nutrition.Further research is needed to assess the effects of AEO at lower doses on these parameters and also proteolysis and methanogenesis.

View Article: PubMed Central - PubMed

Affiliation: Department of Animal Science, Faculty of Agriculture, Bu-Ali Sina University, Hamedan, Iran.

ABSTRACT

Background and objectives: Essential oils may improve the utilization of nutrients by ruminal microorganisms. The aim of this study was to assess the effect of different doses of ajowan essential oils (AEO) on growth and fibrolytic activity of anaerobic fungi, and generic distribution of ciliated protozoa (in vitro).

Material and methods: Different doses of AEO (0, 150, 300, 450 and 600 ppm) were added to experimental tubes. The effect of AEO was evaluated on growth and fibrolytic enzyme activity of an anaerobic fungus (Neocalimastix spp.). Generic distribution of ciliated protozoa were evaluated in response to different doses of AEO.

Results and conclusion: The growth of fungus (Neocalimastix spp.) were inhibited and activity of fibrolytic enzymes of fungus were reduced by adding AEO. Also, an inhibitory effect was seen in concentration of ciliated protozoa and some genus were completely disappeared at the doses of 300 ppm and higher. The doses used in this study reduced the fibrolytic activity of the studied rumen microorganisms which is undesirable in practical animal nutrition. Further research is needed to assess the effects of AEO at lower doses on these parameters and also proteolysis and methanogenesis.

No MeSH data available.