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Detection of virulence genes (bvfA, virB and ure) in Brucella melitensis isolated from aborted fetuses of sheep and goats.

Derakhshandeh A, Firouzi R, Goudarztalejerd A - Iran J Microbiol (2013)

Bottom Line: PCR assay was performed in order to detect the virB, bvfA, and ure genes using specific primers.The frequency of bvfA, virB, and ure genes was 78.50%, 73.80%, and 88.09% among all isolates respectively.The results of the present study showed that most Brucella isolates from this region have virulence factors genes (virB, bvfA, ure) in their genome, and most B. melitensis had ure genes that has been hypothesized to play a role in the pathogenesis of disease.

View Article: PubMed Central - PubMed

Affiliation: Department of Pathobiology, School of Veterinary Medicine, Shiraz University, Shiraz, Iran.

ABSTRACT

Background and objectives: Brucella, causative of brucellosis, has some potential virulence factors involved in Brucella replication and its strategies to circumvent the immune response. One of them is the virB gene that encodes the type IV secretion system proteins (T4SS) involved in intracellular replication of organism. Brucella virulence factor A (bvfA), and urease (ure) has also been described as being implicated in survival, and virulence in the hosts. The aim of this study was to investigate the B. melitensis virulence factor genes among Brucella isolated from aborted fetuses of sheep and goats in Fars province, southern Iran.

Materials and methods: A total of 42 isolates of B. melitensis isolated from aborted fetuses between 2005-2011 in Fars province of Iran was used in this study. PCR assay was performed in order to detect the virB, bvfA, and ure genes using specific primers.

Results and conclusions: The frequency of bvfA, virB, and ure genes was 78.50%, 73.80%, and 88.09% among all isolates respectively. The results of the present study showed that most Brucella isolates from this region have virulence factors genes (virB, bvfA, ure) in their genome, and most B. melitensis had ure genes that has been hypothesized to play a role in the pathogenesis of disease.

No MeSH data available.


Related in: MedlinePlus

Agarose gel electrophoresis of PCR product of three brucella virulence genes. Lane M, 100 bp ladder, lanes 1- 3: PCR product of ure gene: 9-PCR product of virB gene, lanes 7: 6-product of bvfA gene, lane N, Negative control, lanes 4
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Figure 1: Agarose gel electrophoresis of PCR product of three brucella virulence genes. Lane M, 100 bp ladder, lanes 1- 3: PCR product of ure gene: 9-PCR product of virB gene, lanes 7: 6-product of bvfA gene, lane N, Negative control, lanes 4

Mentions: DNA was successfully extracted from all 42 B.melitensis isolates. As expected the bvfA, virB and ure genes assays produced amplicons of 1282, 881 and 2100 bp respectively, (Fig 1). Of 42 B.melitensis isolates; 33 (78.5%) isolates had bvfA genes, 31(73.8%) isolates had virB genes and 37 (88.09) isolates had ure genes.


Detection of virulence genes (bvfA, virB and ure) in Brucella melitensis isolated from aborted fetuses of sheep and goats.

Derakhshandeh A, Firouzi R, Goudarztalejerd A - Iran J Microbiol (2013)

Agarose gel electrophoresis of PCR product of three brucella virulence genes. Lane M, 100 bp ladder, lanes 1- 3: PCR product of ure gene: 9-PCR product of virB gene, lanes 7: 6-product of bvfA gene, lane N, Negative control, lanes 4
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4385168&req=5

Figure 1: Agarose gel electrophoresis of PCR product of three brucella virulence genes. Lane M, 100 bp ladder, lanes 1- 3: PCR product of ure gene: 9-PCR product of virB gene, lanes 7: 6-product of bvfA gene, lane N, Negative control, lanes 4
Mentions: DNA was successfully extracted from all 42 B.melitensis isolates. As expected the bvfA, virB and ure genes assays produced amplicons of 1282, 881 and 2100 bp respectively, (Fig 1). Of 42 B.melitensis isolates; 33 (78.5%) isolates had bvfA genes, 31(73.8%) isolates had virB genes and 37 (88.09) isolates had ure genes.

Bottom Line: PCR assay was performed in order to detect the virB, bvfA, and ure genes using specific primers.The frequency of bvfA, virB, and ure genes was 78.50%, 73.80%, and 88.09% among all isolates respectively.The results of the present study showed that most Brucella isolates from this region have virulence factors genes (virB, bvfA, ure) in their genome, and most B. melitensis had ure genes that has been hypothesized to play a role in the pathogenesis of disease.

View Article: PubMed Central - PubMed

Affiliation: Department of Pathobiology, School of Veterinary Medicine, Shiraz University, Shiraz, Iran.

ABSTRACT

Background and objectives: Brucella, causative of brucellosis, has some potential virulence factors involved in Brucella replication and its strategies to circumvent the immune response. One of them is the virB gene that encodes the type IV secretion system proteins (T4SS) involved in intracellular replication of organism. Brucella virulence factor A (bvfA), and urease (ure) has also been described as being implicated in survival, and virulence in the hosts. The aim of this study was to investigate the B. melitensis virulence factor genes among Brucella isolated from aborted fetuses of sheep and goats in Fars province, southern Iran.

Materials and methods: A total of 42 isolates of B. melitensis isolated from aborted fetuses between 2005-2011 in Fars province of Iran was used in this study. PCR assay was performed in order to detect the virB, bvfA, and ure genes using specific primers.

Results and conclusions: The frequency of bvfA, virB, and ure genes was 78.50%, 73.80%, and 88.09% among all isolates respectively. The results of the present study showed that most Brucella isolates from this region have virulence factors genes (virB, bvfA, ure) in their genome, and most B. melitensis had ure genes that has been hypothesized to play a role in the pathogenesis of disease.

No MeSH data available.


Related in: MedlinePlus