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Comparison of four different culture media for growth of Mycobacterium avium subsp. avium isolated from naturally infected lofts of domestic pigeons.

Mayahi M, Mosavari N, Esmaeilzadeh S, Parvandar-Asadollahi K - Iran J Microbiol (2013)

Bottom Line: Eighty out of more than 600 pigeons were selected based on their clinical signs and poor health conditions.Mycobactin J-supplemented Herrold-egg yolk media yielded greater number of colonies in shorter incubation time in compare with other media.It was concluded that most of the isolates need mycobactin as a growth factor.

View Article: PubMed Central - PubMed

Affiliation: Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran.

ABSTRACT

Background and objectives: Diagnosis of avian tuberculosis by conventional culture method is still considered as the "gold standard" technique. The main objective of this study was to compare growth of Mycobacterium avium subsp. avium on four specific Mycobacterial cultures such as glycerinated Lowenstein-Jensen medium, pyruvate-enriched Lowenstein-Jensen medium, mycobactin J-supplemented Herrold-egg yolk medium and plain Herrold-egg yolk medium.

Materials and methods: Eighty out of more than 600 pigeons were selected based on their clinical signs and poor health conditions. The birds were numbered and their clinical signs were registered in the working sheets, and under standard condi-tion, euthanized, subjecting to necropsy examinations, followed by bacterial culture on four specific media for Mycobacterium avium subsp. avium, including glycerinated Lowenstein-Jensen (LJG) medium, pyruvate-enriched Lowenstein-Jensen medium (LJP), mycobactin J-supplemented Herrold-egg yolk medium and plain Herrold-egg yolk medium.

Results: Fifty one Mycobacterium avium subsp. avium were isolated from pigeons. Mycobactin J-supplemented Herrold-egg yolk media yielded greater number of colonies in shorter incubation time in compare with other media.

Conclusion: It was concluded that most of the isolates need mycobactin as a growth factor.

No MeSH data available.


Related in: MedlinePlus

The example of real PCR amplification product. The 427 bp specific fragment from IS1245. Lane M, DNA size marker (100 base pair ladder). Lane 1 and 2, negative controls (distilled water). Lane 3, negative species control (Mycobacterium bovis AN5 strain, ATCC number 35726). Lane 4, positive control (Mycobacterium avium subsp. avium D4 strain, ATCC number 35713). Lane 5 to 9 samples tested for Mycobacterium avium subsp. avium
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Figure 1: The example of real PCR amplification product. The 427 bp specific fragment from IS1245. Lane M, DNA size marker (100 base pair ladder). Lane 1 and 2, negative controls (distilled water). Lane 3, negative species control (Mycobacterium bovis AN5 strain, ATCC number 35726). Lane 4, positive control (Mycobacterium avium subsp. avium D4 strain, ATCC number 35713). Lane 5 to 9 samples tested for Mycobacterium avium subsp. avium

Mentions: Fifty one AFB were isolated from pigeons. All of the AFB isolates were positive for IS1245 and IS901 in PCR (Figs. 1 and 2). Clinical signs, ecropsy findings, ZN staining and molecular identification confirmed that the pigeons were infected with MAA. In necropsy examinations firm grayish-yellow or grayish-white and raised nodules were found especially on liver and intestines. Liver and intestines were the most frequently affected organs, and lungs were the least affected organs while no macroscopic lesion was found in the gonads, kidneys and CNS. Forty six isolates out of 51 MAA isolates, first appeared on the HM media and then on the other media but with lesser colony significantly. Five out of 51 MAA isolates first appeared on the LJP media, then on the other media. The type of growth was also distinctive for each solid medium. The colonies in HM media were very small, usually in a high number distributed throughout the entire surface of the medium, and difficult to be observed macroscopically compared with the colonies in other culture media. In Lowenstein-Jensen medium, especially pyruvate-enriched, the colonies are rougher, larger, and more easily observed macroscopically. With the exception of 5 isolates, all MAA isolates grew on the HM media in less than 12 weeks incubation, but most of them grew on the other media after 12 weeks.


Comparison of four different culture media for growth of Mycobacterium avium subsp. avium isolated from naturally infected lofts of domestic pigeons.

Mayahi M, Mosavari N, Esmaeilzadeh S, Parvandar-Asadollahi K - Iran J Microbiol (2013)

The example of real PCR amplification product. The 427 bp specific fragment from IS1245. Lane M, DNA size marker (100 base pair ladder). Lane 1 and 2, negative controls (distilled water). Lane 3, negative species control (Mycobacterium bovis AN5 strain, ATCC number 35726). Lane 4, positive control (Mycobacterium avium subsp. avium D4 strain, ATCC number 35713). Lane 5 to 9 samples tested for Mycobacterium avium subsp. avium
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4385164&req=5

Figure 1: The example of real PCR amplification product. The 427 bp specific fragment from IS1245. Lane M, DNA size marker (100 base pair ladder). Lane 1 and 2, negative controls (distilled water). Lane 3, negative species control (Mycobacterium bovis AN5 strain, ATCC number 35726). Lane 4, positive control (Mycobacterium avium subsp. avium D4 strain, ATCC number 35713). Lane 5 to 9 samples tested for Mycobacterium avium subsp. avium
Mentions: Fifty one AFB were isolated from pigeons. All of the AFB isolates were positive for IS1245 and IS901 in PCR (Figs. 1 and 2). Clinical signs, ecropsy findings, ZN staining and molecular identification confirmed that the pigeons were infected with MAA. In necropsy examinations firm grayish-yellow or grayish-white and raised nodules were found especially on liver and intestines. Liver and intestines were the most frequently affected organs, and lungs were the least affected organs while no macroscopic lesion was found in the gonads, kidneys and CNS. Forty six isolates out of 51 MAA isolates, first appeared on the HM media and then on the other media but with lesser colony significantly. Five out of 51 MAA isolates first appeared on the LJP media, then on the other media. The type of growth was also distinctive for each solid medium. The colonies in HM media were very small, usually in a high number distributed throughout the entire surface of the medium, and difficult to be observed macroscopically compared with the colonies in other culture media. In Lowenstein-Jensen medium, especially pyruvate-enriched, the colonies are rougher, larger, and more easily observed macroscopically. With the exception of 5 isolates, all MAA isolates grew on the HM media in less than 12 weeks incubation, but most of them grew on the other media after 12 weeks.

Bottom Line: Eighty out of more than 600 pigeons were selected based on their clinical signs and poor health conditions.Mycobactin J-supplemented Herrold-egg yolk media yielded greater number of colonies in shorter incubation time in compare with other media.It was concluded that most of the isolates need mycobactin as a growth factor.

View Article: PubMed Central - PubMed

Affiliation: Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran.

ABSTRACT

Background and objectives: Diagnosis of avian tuberculosis by conventional culture method is still considered as the "gold standard" technique. The main objective of this study was to compare growth of Mycobacterium avium subsp. avium on four specific Mycobacterial cultures such as glycerinated Lowenstein-Jensen medium, pyruvate-enriched Lowenstein-Jensen medium, mycobactin J-supplemented Herrold-egg yolk medium and plain Herrold-egg yolk medium.

Materials and methods: Eighty out of more than 600 pigeons were selected based on their clinical signs and poor health conditions. The birds were numbered and their clinical signs were registered in the working sheets, and under standard condi-tion, euthanized, subjecting to necropsy examinations, followed by bacterial culture on four specific media for Mycobacterium avium subsp. avium, including glycerinated Lowenstein-Jensen (LJG) medium, pyruvate-enriched Lowenstein-Jensen medium (LJP), mycobactin J-supplemented Herrold-egg yolk medium and plain Herrold-egg yolk medium.

Results: Fifty one Mycobacterium avium subsp. avium were isolated from pigeons. Mycobactin J-supplemented Herrold-egg yolk media yielded greater number of colonies in shorter incubation time in compare with other media.

Conclusion: It was concluded that most of the isolates need mycobactin as a growth factor.

No MeSH data available.


Related in: MedlinePlus