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Screening of soil actinomyectes against Salmonella serovar Typhi NCTC 5761 and characterization of the prominent active strains.

Goljanian Tabrizi S, Hamedi J, Mohammadipanah F - Iran J Microbiol (2013)

Bottom Line: Our anti-Salmonella screening program resulted in nine highly active actinomycete isolates.Three of the producing strains including the isolates HG87, HG116 and HG443 with inhibition zone of >20 mm, were selected for further identification and investigation of cytotoxic effects.Our results demonstrate the broad-range biological activity exhibited by bioactive compounds of soil actinomycetes from Iran.

View Article: PubMed Central - PubMed

Affiliation: Microbial Biotechnology Laboratory, Department of Microbial Biotechnology, School of Biology and Center of Excellence in Phylogeny of Living Organisms, College of Science, University of Tehran, 14155-6455, Tehran, Iran ; University of Tehran Microorganisms Collection (UTMC), Microbial Technology and Products Research Center, University of Tehran, Tehran, Iran.

ABSTRACT

Background and objectives: Annual incidence of infection with S. Typhi is estimated to be about 17 million cases worldwide. A systematic search among actinomycete isolates from soil of Iran aimed at finding active actinomycetes against the causative agent of typhoid fever, Salmonella Typhi was carried out during this study.

Materials and methods: Our anti-Salmonella screening program resulted in nine highly active actinomycete isolates. All nine antibiotic producing strains showed broad-spectrum antibacterial activity, as five strains showed antifungal activity as well. Based on microscopic morphology and cell wall analysis, all nine active actinomycete strains were representatives of the genus Streptomyces. Three of the producing strains including the isolates HG87, HG116 and HG443 with inhibition zone of >20 mm, were selected for further identification and investigation of cytotoxic effects.

Results and conclusion: None of the producing strains showed cytotoxicity on HEK and USSC cell lines, while strain HG116 showed excellent antitumor activity on T47D cancer cell lines. Isolates HG87, HG116 and HG443 can be distinguished from the related species by some phenotypic and biochemical characteristics. Our results demonstrate the broad-range biological activity exhibited by bioactive compounds of soil actinomycetes from Iran.

No MeSH data available.


Related in: MedlinePlus

Phylogenetic trees of the 16S rRNA sequences of the selected strains A, Streptomyces sp. UTMC 01390, B, Streptomyces sp. UTMC 01392 and C, Streptomyces sp. UTMC 01397 (Bootstrap values under 50 are not shown)
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Figure 3: Phylogenetic trees of the 16S rRNA sequences of the selected strains A, Streptomyces sp. UTMC 01390, B, Streptomyces sp. UTMC 01392 and C, Streptomyces sp. UTMC 01397 (Bootstrap values under 50 are not shown)

Mentions: Comparison of an almost complete 16S rRNA sequence of the selected strains with that of Streptomyces species deposited in GenBank and EzTaxon database was done. The 16S rRNA sequence analysis of HG87 revealed 99.92% similarity to S. xinghaiensis. The other similar species to HG87 were S. feralitis and S. tateyamensis sharing 97.91% identity to the strain. HG116 shared 100% resemblance to S. griseoincarnatus, S. labedae, S. variabilis and S. erythrogriseus and HG443 was found to be most similar to S. toxytricini and S. globosus with a sequence identity of 100%. The selected strains HG87, HG116 and HG443 were deposited as Streptomyces sp. UTMC 01390 (GenBank accession number JX291534), Streptomyces sp. UTMC 01392 (GenBank accession number JX291533) and Streptomyces sp. UTMC 01397 (GenBank accession number JX291535), respectively and are accessible in University of Tehran Microorganisms Collection. The phylogenetic trees of the selected strains depict the evolutionary relationships between different Streptomyces species and the selected strains of this screening program (Fig. 3).


Screening of soil actinomyectes against Salmonella serovar Typhi NCTC 5761 and characterization of the prominent active strains.

Goljanian Tabrizi S, Hamedi J, Mohammadipanah F - Iran J Microbiol (2013)

Phylogenetic trees of the 16S rRNA sequences of the selected strains A, Streptomyces sp. UTMC 01390, B, Streptomyces sp. UTMC 01392 and C, Streptomyces sp. UTMC 01397 (Bootstrap values under 50 are not shown)
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4385161&req=5

Figure 3: Phylogenetic trees of the 16S rRNA sequences of the selected strains A, Streptomyces sp. UTMC 01390, B, Streptomyces sp. UTMC 01392 and C, Streptomyces sp. UTMC 01397 (Bootstrap values under 50 are not shown)
Mentions: Comparison of an almost complete 16S rRNA sequence of the selected strains with that of Streptomyces species deposited in GenBank and EzTaxon database was done. The 16S rRNA sequence analysis of HG87 revealed 99.92% similarity to S. xinghaiensis. The other similar species to HG87 were S. feralitis and S. tateyamensis sharing 97.91% identity to the strain. HG116 shared 100% resemblance to S. griseoincarnatus, S. labedae, S. variabilis and S. erythrogriseus and HG443 was found to be most similar to S. toxytricini and S. globosus with a sequence identity of 100%. The selected strains HG87, HG116 and HG443 were deposited as Streptomyces sp. UTMC 01390 (GenBank accession number JX291534), Streptomyces sp. UTMC 01392 (GenBank accession number JX291533) and Streptomyces sp. UTMC 01397 (GenBank accession number JX291535), respectively and are accessible in University of Tehran Microorganisms Collection. The phylogenetic trees of the selected strains depict the evolutionary relationships between different Streptomyces species and the selected strains of this screening program (Fig. 3).

Bottom Line: Our anti-Salmonella screening program resulted in nine highly active actinomycete isolates.Three of the producing strains including the isolates HG87, HG116 and HG443 with inhibition zone of >20 mm, were selected for further identification and investigation of cytotoxic effects.Our results demonstrate the broad-range biological activity exhibited by bioactive compounds of soil actinomycetes from Iran.

View Article: PubMed Central - PubMed

Affiliation: Microbial Biotechnology Laboratory, Department of Microbial Biotechnology, School of Biology and Center of Excellence in Phylogeny of Living Organisms, College of Science, University of Tehran, 14155-6455, Tehran, Iran ; University of Tehran Microorganisms Collection (UTMC), Microbial Technology and Products Research Center, University of Tehran, Tehran, Iran.

ABSTRACT

Background and objectives: Annual incidence of infection with S. Typhi is estimated to be about 17 million cases worldwide. A systematic search among actinomycete isolates from soil of Iran aimed at finding active actinomycetes against the causative agent of typhoid fever, Salmonella Typhi was carried out during this study.

Materials and methods: Our anti-Salmonella screening program resulted in nine highly active actinomycete isolates. All nine antibiotic producing strains showed broad-spectrum antibacterial activity, as five strains showed antifungal activity as well. Based on microscopic morphology and cell wall analysis, all nine active actinomycete strains were representatives of the genus Streptomyces. Three of the producing strains including the isolates HG87, HG116 and HG443 with inhibition zone of >20 mm, were selected for further identification and investigation of cytotoxic effects.

Results and conclusion: None of the producing strains showed cytotoxicity on HEK and USSC cell lines, while strain HG116 showed excellent antitumor activity on T47D cancer cell lines. Isolates HG87, HG116 and HG443 can be distinguished from the related species by some phenotypic and biochemical characteristics. Our results demonstrate the broad-range biological activity exhibited by bioactive compounds of soil actinomycetes from Iran.

No MeSH data available.


Related in: MedlinePlus