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Screening of soil actinomyectes against Salmonella serovar Typhi NCTC 5761 and characterization of the prominent active strains.

Goljanian Tabrizi S, Hamedi J, Mohammadipanah F - Iran J Microbiol (2013)

Bottom Line: Our anti-Salmonella screening program resulted in nine highly active actinomycete isolates.Three of the producing strains including the isolates HG87, HG116 and HG443 with inhibition zone of >20 mm, were selected for further identification and investigation of cytotoxic effects.Our results demonstrate the broad-range biological activity exhibited by bioactive compounds of soil actinomycetes from Iran.

View Article: PubMed Central - PubMed

Affiliation: Microbial Biotechnology Laboratory, Department of Microbial Biotechnology, School of Biology and Center of Excellence in Phylogeny of Living Organisms, College of Science, University of Tehran, 14155-6455, Tehran, Iran ; University of Tehran Microorganisms Collection (UTMC), Microbial Technology and Products Research Center, University of Tehran, Tehran, Iran.

ABSTRACT

Background and objectives: Annual incidence of infection with S. Typhi is estimated to be about 17 million cases worldwide. A systematic search among actinomycete isolates from soil of Iran aimed at finding active actinomycetes against the causative agent of typhoid fever, Salmonella Typhi was carried out during this study.

Materials and methods: Our anti-Salmonella screening program resulted in nine highly active actinomycete isolates. All nine antibiotic producing strains showed broad-spectrum antibacterial activity, as five strains showed antifungal activity as well. Based on microscopic morphology and cell wall analysis, all nine active actinomycete strains were representatives of the genus Streptomyces. Three of the producing strains including the isolates HG87, HG116 and HG443 with inhibition zone of >20 mm, were selected for further identification and investigation of cytotoxic effects.

Results and conclusion: None of the producing strains showed cytotoxicity on HEK and USSC cell lines, while strain HG116 showed excellent antitumor activity on T47D cancer cell lines. Isolates HG87, HG116 and HG443 can be distinguished from the related species by some phenotypic and biochemical characteristics. Our results demonstrate the broad-range biological activity exhibited by bioactive compounds of soil actinomycetes from Iran.

No MeSH data available.


Related in: MedlinePlus

Anti-S. typhi activity of some actinomycetes isolates; the numbers of potent strains are presented on the images
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Related In: Results  -  Collection


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Figure 1: Anti-S. typhi activity of some actinomycetes isolates; the numbers of potent strains are presented on the images

Mentions: From 37 soil samples collected from different regions of Iran, 522 colonies were isolated and screened for their bioactivity against S. Typhi. Nine of the isolated strains had potent antibiotic activity against S. Typhi (Fig. 1). The activity of these isolates was also assayed against other Gram negative and Gram positive representatives. All of the nine active strains exhibited broad spectrum antibacterial activities. Activity against S. Typhi and other Gram negative test strains was less than that of against Gram positive test strains (Table 1). The morphological characteristics and the type of the diaminopimelic acid (L-DAP) of all of the bioactive strains were consistent with their classification in the genus Streptomyces (20).


Screening of soil actinomyectes against Salmonella serovar Typhi NCTC 5761 and characterization of the prominent active strains.

Goljanian Tabrizi S, Hamedi J, Mohammadipanah F - Iran J Microbiol (2013)

Anti-S. typhi activity of some actinomycetes isolates; the numbers of potent strains are presented on the images
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4385161&req=5

Figure 1: Anti-S. typhi activity of some actinomycetes isolates; the numbers of potent strains are presented on the images
Mentions: From 37 soil samples collected from different regions of Iran, 522 colonies were isolated and screened for their bioactivity against S. Typhi. Nine of the isolated strains had potent antibiotic activity against S. Typhi (Fig. 1). The activity of these isolates was also assayed against other Gram negative and Gram positive representatives. All of the nine active strains exhibited broad spectrum antibacterial activities. Activity against S. Typhi and other Gram negative test strains was less than that of against Gram positive test strains (Table 1). The morphological characteristics and the type of the diaminopimelic acid (L-DAP) of all of the bioactive strains were consistent with their classification in the genus Streptomyces (20).

Bottom Line: Our anti-Salmonella screening program resulted in nine highly active actinomycete isolates.Three of the producing strains including the isolates HG87, HG116 and HG443 with inhibition zone of >20 mm, were selected for further identification and investigation of cytotoxic effects.Our results demonstrate the broad-range biological activity exhibited by bioactive compounds of soil actinomycetes from Iran.

View Article: PubMed Central - PubMed

Affiliation: Microbial Biotechnology Laboratory, Department of Microbial Biotechnology, School of Biology and Center of Excellence in Phylogeny of Living Organisms, College of Science, University of Tehran, 14155-6455, Tehran, Iran ; University of Tehran Microorganisms Collection (UTMC), Microbial Technology and Products Research Center, University of Tehran, Tehran, Iran.

ABSTRACT

Background and objectives: Annual incidence of infection with S. Typhi is estimated to be about 17 million cases worldwide. A systematic search among actinomycete isolates from soil of Iran aimed at finding active actinomycetes against the causative agent of typhoid fever, Salmonella Typhi was carried out during this study.

Materials and methods: Our anti-Salmonella screening program resulted in nine highly active actinomycete isolates. All nine antibiotic producing strains showed broad-spectrum antibacterial activity, as five strains showed antifungal activity as well. Based on microscopic morphology and cell wall analysis, all nine active actinomycete strains were representatives of the genus Streptomyces. Three of the producing strains including the isolates HG87, HG116 and HG443 with inhibition zone of >20 mm, were selected for further identification and investigation of cytotoxic effects.

Results and conclusion: None of the producing strains showed cytotoxicity on HEK and USSC cell lines, while strain HG116 showed excellent antitumor activity on T47D cancer cell lines. Isolates HG87, HG116 and HG443 can be distinguished from the related species by some phenotypic and biochemical characteristics. Our results demonstrate the broad-range biological activity exhibited by bioactive compounds of soil actinomycetes from Iran.

No MeSH data available.


Related in: MedlinePlus