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Genotyping of carbapenem resistant Acinetobacter baumannii isolated from tracheal tube discharge of hospitalized patients in intensive care units, Ahvaz, Iran.

Shoja S, Moosavian M, Peymani A, Tabatabaiefar MA, Rostami S, Ebrahimi N - Iran J Microbiol (2013)

Bottom Line: Genotype B, and C were found in 28.6% and 27.7%, respectively.Based on the obtained results, the rate of carbapenem resistance was high among of A. baumannii which was isolated from intensive care units patients and oxacillinase genes were the most prevalent carbapenem resistant genes.These results revealed that three clones, A, B and C of A.baumannii are common in our hospitals.

View Article: PubMed Central - PubMed

Affiliation: Department of Microbiology, School of Medicine, Infectious & Tropical Diseases Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.

ABSTRACT

Background and objectives: Carbapenem resistant A. baumannii is an emerging cause of nosocomial infections. The aims of this study were identification of the most prevalent of carbapenem resistant genes, molecular typing and antimicrobial evaluation of A.baumannii in intensive care units.

Materials and methods: Two hundred and six A. baumannii were isolated from tracheal tube discharge of hospitalized patients at different intensive care units in Ahvaz, Iran. Antimicrobial susceptibility test was done on all isolates. Multiplex and singleplex PCR were performed for detection of bla OXA-23-like, bla OXA-24-like, bla OXA-51-like, bla OXA-58-like, bla VIM, bla IMP, bla SPM and bla NDM genes. Genetic relationship of all isolates was determined by REP-PCR method.

Results: Out of 206 examined isolates, 198 (96.1%) isolates were resistant to imipenem and meropenem. However 3.9% isolates were sensitive to these antibiotics. The bla OXA-23-like and bla OXA-24-like genes were detected in 85% and 8.7% of strains, respectively. No bla OXA-58- like, bla IMP, bla VIM, bla SPM and bla NDM were detected. REP-PCR results showed that isolates were belonged to five genotypes: Genotype A was the most prevalent (P- value < 0.001): it was observed in 75 of 206 strains (36.4%). Genotype B, and C were found in 28.6% and 27.7%, respectively. The rate of other genotypes was as follows: D (2.4%), E (1%).

Conclusion: Based on the obtained results, the rate of carbapenem resistance was high among of A. baumannii which was isolated from intensive care units patients and oxacillinase genes were the most prevalent carbapenem resistant genes. These results revealed that three clones, A, B and C of A.baumannii are common in our hospitals.

No MeSH data available.


Related in: MedlinePlus

Electrophoresis of REP-PCR products for three major clone of CRAB. Double fingerprints have been shown for each isolate. Lanes 1, 6 , 11, 1 kb DNA ladder. Lanes 2, 3 (clone A), lanes 4, 5 (clone B), lanes 7, 8 (clone C), Lane 9, negative control and lane 10, A. baumanniiNCTC 12156 (ATCC 19606).
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Figure 2: Electrophoresis of REP-PCR products for three major clone of CRAB. Double fingerprints have been shown for each isolate. Lanes 1, 6 , 11, 1 kb DNA ladder. Lanes 2, 3 (clone A), lanes 4, 5 (clone B), lanes 7, 8 (clone C), Lane 9, negative control and lane 10, A. baumanniiNCTC 12156 (ATCC 19606).

Mentions: To determine the genetic relationship, all isolates were analyzed by REP-PCR. According to the REP-PCR results, carbapenem resistant isolates were grouped into five clones. The first prevalent genotype was named genotype A and was observed in 75 (36.4%) of 206 isolates (P- value < 0.001), thereafter 59 isolates (28.6%) and 57 isolates (27.7%) belonged to genotypes B and C, respectively, (Fig. 2). The rates of other genotypes were as follows: D: 5 (2.4%), E: 2 (1%). All clones were distributed throughout the study period. Albeit blaOXA-23-like was found in all clones, blaOXA-24-like was seen only in clones A, B, C and D, but clones E was negative for this gene. Eight isolates (3.9%) were carbapenem susceptible and showed different REP pattern from each other. Frequency of each clone in different ICUs was presented in Table 3.


Genotyping of carbapenem resistant Acinetobacter baumannii isolated from tracheal tube discharge of hospitalized patients in intensive care units, Ahvaz, Iran.

Shoja S, Moosavian M, Peymani A, Tabatabaiefar MA, Rostami S, Ebrahimi N - Iran J Microbiol (2013)

Electrophoresis of REP-PCR products for three major clone of CRAB. Double fingerprints have been shown for each isolate. Lanes 1, 6 , 11, 1 kb DNA ladder. Lanes 2, 3 (clone A), lanes 4, 5 (clone B), lanes 7, 8 (clone C), Lane 9, negative control and lane 10, A. baumanniiNCTC 12156 (ATCC 19606).
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Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4385154&req=5

Figure 2: Electrophoresis of REP-PCR products for three major clone of CRAB. Double fingerprints have been shown for each isolate. Lanes 1, 6 , 11, 1 kb DNA ladder. Lanes 2, 3 (clone A), lanes 4, 5 (clone B), lanes 7, 8 (clone C), Lane 9, negative control and lane 10, A. baumanniiNCTC 12156 (ATCC 19606).
Mentions: To determine the genetic relationship, all isolates were analyzed by REP-PCR. According to the REP-PCR results, carbapenem resistant isolates were grouped into five clones. The first prevalent genotype was named genotype A and was observed in 75 (36.4%) of 206 isolates (P- value < 0.001), thereafter 59 isolates (28.6%) and 57 isolates (27.7%) belonged to genotypes B and C, respectively, (Fig. 2). The rates of other genotypes were as follows: D: 5 (2.4%), E: 2 (1%). All clones were distributed throughout the study period. Albeit blaOXA-23-like was found in all clones, blaOXA-24-like was seen only in clones A, B, C and D, but clones E was negative for this gene. Eight isolates (3.9%) were carbapenem susceptible and showed different REP pattern from each other. Frequency of each clone in different ICUs was presented in Table 3.

Bottom Line: Genotype B, and C were found in 28.6% and 27.7%, respectively.Based on the obtained results, the rate of carbapenem resistance was high among of A. baumannii which was isolated from intensive care units patients and oxacillinase genes were the most prevalent carbapenem resistant genes.These results revealed that three clones, A, B and C of A.baumannii are common in our hospitals.

View Article: PubMed Central - PubMed

Affiliation: Department of Microbiology, School of Medicine, Infectious & Tropical Diseases Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.

ABSTRACT

Background and objectives: Carbapenem resistant A. baumannii is an emerging cause of nosocomial infections. The aims of this study were identification of the most prevalent of carbapenem resistant genes, molecular typing and antimicrobial evaluation of A.baumannii in intensive care units.

Materials and methods: Two hundred and six A. baumannii were isolated from tracheal tube discharge of hospitalized patients at different intensive care units in Ahvaz, Iran. Antimicrobial susceptibility test was done on all isolates. Multiplex and singleplex PCR were performed for detection of bla OXA-23-like, bla OXA-24-like, bla OXA-51-like, bla OXA-58-like, bla VIM, bla IMP, bla SPM and bla NDM genes. Genetic relationship of all isolates was determined by REP-PCR method.

Results: Out of 206 examined isolates, 198 (96.1%) isolates were resistant to imipenem and meropenem. However 3.9% isolates were sensitive to these antibiotics. The bla OXA-23-like and bla OXA-24-like genes were detected in 85% and 8.7% of strains, respectively. No bla OXA-58- like, bla IMP, bla VIM, bla SPM and bla NDM were detected. REP-PCR results showed that isolates were belonged to five genotypes: Genotype A was the most prevalent (P- value < 0.001): it was observed in 75 of 206 strains (36.4%). Genotype B, and C were found in 28.6% and 27.7%, respectively. The rate of other genotypes was as follows: D (2.4%), E (1%).

Conclusion: Based on the obtained results, the rate of carbapenem resistance was high among of A. baumannii which was isolated from intensive care units patients and oxacillinase genes were the most prevalent carbapenem resistant genes. These results revealed that three clones, A, B and C of A.baumannii are common in our hospitals.

No MeSH data available.


Related in: MedlinePlus