Limits...
Genotyping of carbapenem resistant Acinetobacter baumannii isolated from tracheal tube discharge of hospitalized patients in intensive care units, Ahvaz, Iran.

Shoja S, Moosavian M, Peymani A, Tabatabaiefar MA, Rostami S, Ebrahimi N - Iran J Microbiol (2013)

Bottom Line: Genotype B, and C were found in 28.6% and 27.7%, respectively.Based on the obtained results, the rate of carbapenem resistance was high among of A. baumannii which was isolated from intensive care units patients and oxacillinase genes were the most prevalent carbapenem resistant genes.These results revealed that three clones, A, B and C of A.baumannii are common in our hospitals.

View Article: PubMed Central - PubMed

Affiliation: Department of Microbiology, School of Medicine, Infectious & Tropical Diseases Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.

ABSTRACT

Background and objectives: Carbapenem resistant A. baumannii is an emerging cause of nosocomial infections. The aims of this study were identification of the most prevalent of carbapenem resistant genes, molecular typing and antimicrobial evaluation of A.baumannii in intensive care units.

Materials and methods: Two hundred and six A. baumannii were isolated from tracheal tube discharge of hospitalized patients at different intensive care units in Ahvaz, Iran. Antimicrobial susceptibility test was done on all isolates. Multiplex and singleplex PCR were performed for detection of bla OXA-23-like, bla OXA-24-like, bla OXA-51-like, bla OXA-58-like, bla VIM, bla IMP, bla SPM and bla NDM genes. Genetic relationship of all isolates was determined by REP-PCR method.

Results: Out of 206 examined isolates, 198 (96.1%) isolates were resistant to imipenem and meropenem. However 3.9% isolates were sensitive to these antibiotics. The bla OXA-23-like and bla OXA-24-like genes were detected in 85% and 8.7% of strains, respectively. No bla OXA-58- like, bla IMP, bla VIM, bla SPM and bla NDM were detected. REP-PCR results showed that isolates were belonged to five genotypes: Genotype A was the most prevalent (P- value < 0.001): it was observed in 75 of 206 strains (36.4%). Genotype B, and C were found in 28.6% and 27.7%, respectively. The rate of other genotypes was as follows: D (2.4%), E (1%).

Conclusion: Based on the obtained results, the rate of carbapenem resistance was high among of A. baumannii which was isolated from intensive care units patients and oxacillinase genes were the most prevalent carbapenem resistant genes. These results revealed that three clones, A, B and C of A.baumannii are common in our hospitals.

No MeSH data available.


Related in: MedlinePlus

Electrophoresis results of multiplex PCR. Lanes 1 and 14: 100bp DNA ladder. Lanes 2, 7, 8: isolates with blaOXA-24-like in 249 bp. Lanes 3, 4, 5, 9 & 10: isolates with blaOXA-23-like in 501 bp. Lanes 6 & 11 isolates that are negative for blaOXA-23-like, blaOXA-24-like and blaOXA-58-like. Lane 12 negative control (distilled water ) and lane 13 Positive control (A. baumannii NCTC 13304, NCTC 13302, NCTC 13305 used as positive control for blaOXA-23-like, blaOXA-24-like and blaOXA-58-like respectively.
© Copyright Policy
Related In: Results  -  Collection


getmorefigures.php?uid=PMC4385154&req=5

Figure 1: Electrophoresis results of multiplex PCR. Lanes 1 and 14: 100bp DNA ladder. Lanes 2, 7, 8: isolates with blaOXA-24-like in 249 bp. Lanes 3, 4, 5, 9 & 10: isolates with blaOXA-23-like in 501 bp. Lanes 6 & 11 isolates that are negative for blaOXA-23-like, blaOXA-24-like and blaOXA-58-like. Lane 12 negative control (distilled water ) and lane 13 Positive control (A. baumannii NCTC 13304, NCTC 13302, NCTC 13305 used as positive control for blaOXA-23-like, blaOXA-24-like and blaOXA-58-like respectively.

Mentions: Results of multiplex PCR showed the blaOXA-23-likeand blaOXA-24-like genes were detected in 85% and 8.7%, respectively (Fig. 1). Six isolates were carbapenem resistant but these isolates were negative for blaOXA-23-like, blaOXA-24-like, blaOXA-58-like and studied metallo-β-lactamase genes. No blaOXA-58-like, blaIMP, blaVIM, blaSPM and blaNDM were detected. blaOXA-23-like, blaOXA-24-likeand blaOXA-58-likeblaIMP, blaVIM, blaSPM and blaNDM were not detected in carbapenem susceptible A. baumannii (CSAB) isolates.


Genotyping of carbapenem resistant Acinetobacter baumannii isolated from tracheal tube discharge of hospitalized patients in intensive care units, Ahvaz, Iran.

Shoja S, Moosavian M, Peymani A, Tabatabaiefar MA, Rostami S, Ebrahimi N - Iran J Microbiol (2013)

Electrophoresis results of multiplex PCR. Lanes 1 and 14: 100bp DNA ladder. Lanes 2, 7, 8: isolates with blaOXA-24-like in 249 bp. Lanes 3, 4, 5, 9 & 10: isolates with blaOXA-23-like in 501 bp. Lanes 6 & 11 isolates that are negative for blaOXA-23-like, blaOXA-24-like and blaOXA-58-like. Lane 12 negative control (distilled water ) and lane 13 Positive control (A. baumannii NCTC 13304, NCTC 13302, NCTC 13305 used as positive control for blaOXA-23-like, blaOXA-24-like and blaOXA-58-like respectively.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4385154&req=5

Figure 1: Electrophoresis results of multiplex PCR. Lanes 1 and 14: 100bp DNA ladder. Lanes 2, 7, 8: isolates with blaOXA-24-like in 249 bp. Lanes 3, 4, 5, 9 & 10: isolates with blaOXA-23-like in 501 bp. Lanes 6 & 11 isolates that are negative for blaOXA-23-like, blaOXA-24-like and blaOXA-58-like. Lane 12 negative control (distilled water ) and lane 13 Positive control (A. baumannii NCTC 13304, NCTC 13302, NCTC 13305 used as positive control for blaOXA-23-like, blaOXA-24-like and blaOXA-58-like respectively.
Mentions: Results of multiplex PCR showed the blaOXA-23-likeand blaOXA-24-like genes were detected in 85% and 8.7%, respectively (Fig. 1). Six isolates were carbapenem resistant but these isolates were negative for blaOXA-23-like, blaOXA-24-like, blaOXA-58-like and studied metallo-β-lactamase genes. No blaOXA-58-like, blaIMP, blaVIM, blaSPM and blaNDM were detected. blaOXA-23-like, blaOXA-24-likeand blaOXA-58-likeblaIMP, blaVIM, blaSPM and blaNDM were not detected in carbapenem susceptible A. baumannii (CSAB) isolates.

Bottom Line: Genotype B, and C were found in 28.6% and 27.7%, respectively.Based on the obtained results, the rate of carbapenem resistance was high among of A. baumannii which was isolated from intensive care units patients and oxacillinase genes were the most prevalent carbapenem resistant genes.These results revealed that three clones, A, B and C of A.baumannii are common in our hospitals.

View Article: PubMed Central - PubMed

Affiliation: Department of Microbiology, School of Medicine, Infectious & Tropical Diseases Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.

ABSTRACT

Background and objectives: Carbapenem resistant A. baumannii is an emerging cause of nosocomial infections. The aims of this study were identification of the most prevalent of carbapenem resistant genes, molecular typing and antimicrobial evaluation of A.baumannii in intensive care units.

Materials and methods: Two hundred and six A. baumannii were isolated from tracheal tube discharge of hospitalized patients at different intensive care units in Ahvaz, Iran. Antimicrobial susceptibility test was done on all isolates. Multiplex and singleplex PCR were performed for detection of bla OXA-23-like, bla OXA-24-like, bla OXA-51-like, bla OXA-58-like, bla VIM, bla IMP, bla SPM and bla NDM genes. Genetic relationship of all isolates was determined by REP-PCR method.

Results: Out of 206 examined isolates, 198 (96.1%) isolates were resistant to imipenem and meropenem. However 3.9% isolates were sensitive to these antibiotics. The bla OXA-23-like and bla OXA-24-like genes were detected in 85% and 8.7% of strains, respectively. No bla OXA-58- like, bla IMP, bla VIM, bla SPM and bla NDM were detected. REP-PCR results showed that isolates were belonged to five genotypes: Genotype A was the most prevalent (P- value < 0.001): it was observed in 75 of 206 strains (36.4%). Genotype B, and C were found in 28.6% and 27.7%, respectively. The rate of other genotypes was as follows: D (2.4%), E (1%).

Conclusion: Based on the obtained results, the rate of carbapenem resistance was high among of A. baumannii which was isolated from intensive care units patients and oxacillinase genes were the most prevalent carbapenem resistant genes. These results revealed that three clones, A, B and C of A.baumannii are common in our hospitals.

No MeSH data available.


Related in: MedlinePlus