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Induction, expression and characterisation of laccase genes from the marine-derived fungal strains Nigrospora sp. CBMAI 1328 and Arthopyrenia sp. CBMAI 1330.

Passarini MR, Ottoni CA, Santos C, Lima N, Sette LD - AMB Express (2015)

Bottom Line: The effect of copper on Lcc gene expression was evaluated by reverse transcriptase polymerase chain reaction.Molecular approaches allowed the detection of Lcc isozymes and suggest the presence of at least two undescribed putative genes.Additionally, Lcc sequences from the both fungal strains clustered with other Lcc sequences from other fungi that inhabit marine environments.

View Article: PubMed Central - PubMed

Affiliation: Divisão de Recursos Microbianos, CPQBA/UNICAMP, CP 6171, 13083-970 Campinas, SP Brazil.

ABSTRACT
The capability of the fungi Nigrospora sp. CBMAI 1328 and Arthopyrenia sp. CBMAI 1330 isolated from marine sponge to synthesise laccases (Lcc) in the presence of the inducer copper (1-10 μM) was assessed. In a liquid culture medium supplemented with 5 μM of copper sulphate after 5 days of incubation, Nigrospora sp. presented the highest Lcc activity (25.2 U·L(-1)). The effect of copper on Lcc gene expression was evaluated by reverse transcriptase polymerase chain reaction. Nigrospora sp. showed the highest gene expression of Lcc under the same conditions of Lcc synthesis. The highest Lcc expression by the Arthopyrenia sp. was detected at 96 h of incubation in absence of copper. Molecular approaches allowed the detection of Lcc isozymes and suggest the presence of at least two undescribed putative genes. Additionally, Lcc sequences from the both fungal strains clustered with other Lcc sequences from other fungi that inhabit marine environments.

No MeSH data available.


Related in: MedlinePlus

Comparative schematics of Lcc genes fromNigrosporasp. CBMAI 1328 (clone A1),Arthopyreniasp. CBMAI 1330 (clones F9 and C9) andClavariopsis aquatic(ACR20672). The red boxes show the possible copper binding regions (I and II).
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Fig4: Comparative schematics of Lcc genes fromNigrosporasp. CBMAI 1328 (clone A1),Arthopyreniasp. CBMAI 1330 (clones F9 and C9) andClavariopsis aquatic(ACR20672). The red boxes show the possible copper binding regions (I and II).

Mentions: Molecular characterisation of Lcc genes from the Nigrospora sp. CBMAI 1328 and Arthopyrenia sp. CBMAI 1330 are showed in Figure 3. Comparative schematics of Lcc genes from both marine-derived fungi and Clavariopsis aquatica are presented in Figure 4.


Induction, expression and characterisation of laccase genes from the marine-derived fungal strains Nigrospora sp. CBMAI 1328 and Arthopyrenia sp. CBMAI 1330.

Passarini MR, Ottoni CA, Santos C, Lima N, Sette LD - AMB Express (2015)

Comparative schematics of Lcc genes fromNigrosporasp. CBMAI 1328 (clone A1),Arthopyreniasp. CBMAI 1330 (clones F9 and C9) andClavariopsis aquatic(ACR20672). The red boxes show the possible copper binding regions (I and II).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4385153&req=5

Fig4: Comparative schematics of Lcc genes fromNigrosporasp. CBMAI 1328 (clone A1),Arthopyreniasp. CBMAI 1330 (clones F9 and C9) andClavariopsis aquatic(ACR20672). The red boxes show the possible copper binding regions (I and II).
Mentions: Molecular characterisation of Lcc genes from the Nigrospora sp. CBMAI 1328 and Arthopyrenia sp. CBMAI 1330 are showed in Figure 3. Comparative schematics of Lcc genes from both marine-derived fungi and Clavariopsis aquatica are presented in Figure 4.

Bottom Line: The effect of copper on Lcc gene expression was evaluated by reverse transcriptase polymerase chain reaction.Molecular approaches allowed the detection of Lcc isozymes and suggest the presence of at least two undescribed putative genes.Additionally, Lcc sequences from the both fungal strains clustered with other Lcc sequences from other fungi that inhabit marine environments.

View Article: PubMed Central - PubMed

Affiliation: Divisão de Recursos Microbianos, CPQBA/UNICAMP, CP 6171, 13083-970 Campinas, SP Brazil.

ABSTRACT
The capability of the fungi Nigrospora sp. CBMAI 1328 and Arthopyrenia sp. CBMAI 1330 isolated from marine sponge to synthesise laccases (Lcc) in the presence of the inducer copper (1-10 μM) was assessed. In a liquid culture medium supplemented with 5 μM of copper sulphate after 5 days of incubation, Nigrospora sp. presented the highest Lcc activity (25.2 U·L(-1)). The effect of copper on Lcc gene expression was evaluated by reverse transcriptase polymerase chain reaction. Nigrospora sp. showed the highest gene expression of Lcc under the same conditions of Lcc synthesis. The highest Lcc expression by the Arthopyrenia sp. was detected at 96 h of incubation in absence of copper. Molecular approaches allowed the detection of Lcc isozymes and suggest the presence of at least two undescribed putative genes. Additionally, Lcc sequences from the both fungal strains clustered with other Lcc sequences from other fungi that inhabit marine environments.

No MeSH data available.


Related in: MedlinePlus