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Induction, expression and characterisation of laccase genes from the marine-derived fungal strains Nigrospora sp. CBMAI 1328 and Arthopyrenia sp. CBMAI 1330.

Passarini MR, Ottoni CA, Santos C, Lima N, Sette LD - AMB Express (2015)

Bottom Line: The effect of copper on Lcc gene expression was evaluated by reverse transcriptase polymerase chain reaction.Molecular approaches allowed the detection of Lcc isozymes and suggest the presence of at least two undescribed putative genes.Additionally, Lcc sequences from the both fungal strains clustered with other Lcc sequences from other fungi that inhabit marine environments.

View Article: PubMed Central - PubMed

Affiliation: Divisão de Recursos Microbianos, CPQBA/UNICAMP, CP 6171, 13083-970 Campinas, SP Brazil.

ABSTRACT
The capability of the fungi Nigrospora sp. CBMAI 1328 and Arthopyrenia sp. CBMAI 1330 isolated from marine sponge to synthesise laccases (Lcc) in the presence of the inducer copper (1-10 μM) was assessed. In a liquid culture medium supplemented with 5 μM of copper sulphate after 5 days of incubation, Nigrospora sp. presented the highest Lcc activity (25.2 U·L(-1)). The effect of copper on Lcc gene expression was evaluated by reverse transcriptase polymerase chain reaction. Nigrospora sp. showed the highest gene expression of Lcc under the same conditions of Lcc synthesis. The highest Lcc expression by the Arthopyrenia sp. was detected at 96 h of incubation in absence of copper. Molecular approaches allowed the detection of Lcc isozymes and suggest the presence of at least two undescribed putative genes. Additionally, Lcc sequences from the both fungal strains clustered with other Lcc sequences from other fungi that inhabit marine environments.

No MeSH data available.


Related in: MedlinePlus

Phylogenetic tree constructed based on the amino acid alignment of protein sequences of fungal Lcc genes. Bootstrap with 1000 replicates was performed by analysis of P-distance. Values greater than 50% are indicated at the nodes of the branches. The horizontal bar represents a distance of 0.05 amino acid substitutions per site.
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Fig2: Phylogenetic tree constructed based on the amino acid alignment of protein sequences of fungal Lcc genes. Bootstrap with 1000 replicates was performed by analysis of P-distance. Values greater than 50% are indicated at the nodes of the branches. The horizontal bar represents a distance of 0.05 amino acid substitutions per site.

Mentions: Data derived from phylogenetic analyses showed that only the sequence of clone F9 formed a cluster with the Lcc gene from fungus Clavariopsis aquatica. The other two sequences (from clone C9 and A1) clustered together and separated from the sequences recovered from GenBank database (Figure 2).


Induction, expression and characterisation of laccase genes from the marine-derived fungal strains Nigrospora sp. CBMAI 1328 and Arthopyrenia sp. CBMAI 1330.

Passarini MR, Ottoni CA, Santos C, Lima N, Sette LD - AMB Express (2015)

Phylogenetic tree constructed based on the amino acid alignment of protein sequences of fungal Lcc genes. Bootstrap with 1000 replicates was performed by analysis of P-distance. Values greater than 50% are indicated at the nodes of the branches. The horizontal bar represents a distance of 0.05 amino acid substitutions per site.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4385153&req=5

Fig2: Phylogenetic tree constructed based on the amino acid alignment of protein sequences of fungal Lcc genes. Bootstrap with 1000 replicates was performed by analysis of P-distance. Values greater than 50% are indicated at the nodes of the branches. The horizontal bar represents a distance of 0.05 amino acid substitutions per site.
Mentions: Data derived from phylogenetic analyses showed that only the sequence of clone F9 formed a cluster with the Lcc gene from fungus Clavariopsis aquatica. The other two sequences (from clone C9 and A1) clustered together and separated from the sequences recovered from GenBank database (Figure 2).

Bottom Line: The effect of copper on Lcc gene expression was evaluated by reverse transcriptase polymerase chain reaction.Molecular approaches allowed the detection of Lcc isozymes and suggest the presence of at least two undescribed putative genes.Additionally, Lcc sequences from the both fungal strains clustered with other Lcc sequences from other fungi that inhabit marine environments.

View Article: PubMed Central - PubMed

Affiliation: Divisão de Recursos Microbianos, CPQBA/UNICAMP, CP 6171, 13083-970 Campinas, SP Brazil.

ABSTRACT
The capability of the fungi Nigrospora sp. CBMAI 1328 and Arthopyrenia sp. CBMAI 1330 isolated from marine sponge to synthesise laccases (Lcc) in the presence of the inducer copper (1-10 μM) was assessed. In a liquid culture medium supplemented with 5 μM of copper sulphate after 5 days of incubation, Nigrospora sp. presented the highest Lcc activity (25.2 U·L(-1)). The effect of copper on Lcc gene expression was evaluated by reverse transcriptase polymerase chain reaction. Nigrospora sp. showed the highest gene expression of Lcc under the same conditions of Lcc synthesis. The highest Lcc expression by the Arthopyrenia sp. was detected at 96 h of incubation in absence of copper. Molecular approaches allowed the detection of Lcc isozymes and suggest the presence of at least two undescribed putative genes. Additionally, Lcc sequences from the both fungal strains clustered with other Lcc sequences from other fungi that inhabit marine environments.

No MeSH data available.


Related in: MedlinePlus