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Induction, expression and characterisation of laccase genes from the marine-derived fungal strains Nigrospora sp. CBMAI 1328 and Arthopyrenia sp. CBMAI 1330.

Passarini MR, Ottoni CA, Santos C, Lima N, Sette LD - AMB Express (2015)

Bottom Line: The effect of copper on Lcc gene expression was evaluated by reverse transcriptase polymerase chain reaction.Molecular approaches allowed the detection of Lcc isozymes and suggest the presence of at least two undescribed putative genes.Additionally, Lcc sequences from the both fungal strains clustered with other Lcc sequences from other fungi that inhabit marine environments.

View Article: PubMed Central - PubMed

Affiliation: Divisão de Recursos Microbianos, CPQBA/UNICAMP, CP 6171, 13083-970 Campinas, SP Brazil.

ABSTRACT
The capability of the fungi Nigrospora sp. CBMAI 1328 and Arthopyrenia sp. CBMAI 1330 isolated from marine sponge to synthesise laccases (Lcc) in the presence of the inducer copper (1-10 μM) was assessed. In a liquid culture medium supplemented with 5 μM of copper sulphate after 5 days of incubation, Nigrospora sp. presented the highest Lcc activity (25.2 U·L(-1)). The effect of copper on Lcc gene expression was evaluated by reverse transcriptase polymerase chain reaction. Nigrospora sp. showed the highest gene expression of Lcc under the same conditions of Lcc synthesis. The highest Lcc expression by the Arthopyrenia sp. was detected at 96 h of incubation in absence of copper. Molecular approaches allowed the detection of Lcc isozymes and suggest the presence of at least two undescribed putative genes. Additionally, Lcc sequences from the both fungal strains clustered with other Lcc sequences from other fungi that inhabit marine environments.

No MeSH data available.


Related in: MedlinePlus

Detection of Lcc gene expression byNigrosporasp. CBMAI 1328 (a) andArthopyreniasp. CBMAI 1330 (b) in different conditions by using RT-PCR. The Lcc gene expression was determined by the relative intensity based on the ratio between the Lcc gene expressions relative to β-tubulin.
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Fig1: Detection of Lcc gene expression byNigrosporasp. CBMAI 1328 (a) andArthopyreniasp. CBMAI 1330 (b) in different conditions by using RT-PCR. The Lcc gene expression was determined by the relative intensity based on the ratio between the Lcc gene expressions relative to β-tubulin.

Mentions: By using the RT-PCR approach, the levels of Lcc gene expression by the Nigrospora sp. CBMAI 1328 and Arthopyrenia sp. CBMAI 1330 were determined. Laccase gene expression was detected in the presence and absence of CuSO4 (Figure 1). These results allow the inference that these fungi the enzyme is constitutively and inductively expressed. The highest rate of Lcc gene expression by Nigrospora sp. CBMAI 1328 was achieved in the presence of 5 μM CuSO4 after 120 h of incubation in LCM. In these same conditions this fungus produced the higher level of laccase (25.2 U·L−1), as showed in Table 1. For Arthopyrenia sp. CBMAI 1330 higher Lcc gene expression was detected in LCM without supplementation of CuSO4 (control) after 96 h of incubation (Figure 1b). Coincidently, the higher rates of Lcc activity by this fungus were also observed in submerged LMC without CuSO4 after 96 and 120 h of incubation (Table 1).Figure 1


Induction, expression and characterisation of laccase genes from the marine-derived fungal strains Nigrospora sp. CBMAI 1328 and Arthopyrenia sp. CBMAI 1330.

Passarini MR, Ottoni CA, Santos C, Lima N, Sette LD - AMB Express (2015)

Detection of Lcc gene expression byNigrosporasp. CBMAI 1328 (a) andArthopyreniasp. CBMAI 1330 (b) in different conditions by using RT-PCR. The Lcc gene expression was determined by the relative intensity based on the ratio between the Lcc gene expressions relative to β-tubulin.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4385153&req=5

Fig1: Detection of Lcc gene expression byNigrosporasp. CBMAI 1328 (a) andArthopyreniasp. CBMAI 1330 (b) in different conditions by using RT-PCR. The Lcc gene expression was determined by the relative intensity based on the ratio between the Lcc gene expressions relative to β-tubulin.
Mentions: By using the RT-PCR approach, the levels of Lcc gene expression by the Nigrospora sp. CBMAI 1328 and Arthopyrenia sp. CBMAI 1330 were determined. Laccase gene expression was detected in the presence and absence of CuSO4 (Figure 1). These results allow the inference that these fungi the enzyme is constitutively and inductively expressed. The highest rate of Lcc gene expression by Nigrospora sp. CBMAI 1328 was achieved in the presence of 5 μM CuSO4 after 120 h of incubation in LCM. In these same conditions this fungus produced the higher level of laccase (25.2 U·L−1), as showed in Table 1. For Arthopyrenia sp. CBMAI 1330 higher Lcc gene expression was detected in LCM without supplementation of CuSO4 (control) after 96 h of incubation (Figure 1b). Coincidently, the higher rates of Lcc activity by this fungus were also observed in submerged LMC without CuSO4 after 96 and 120 h of incubation (Table 1).Figure 1

Bottom Line: The effect of copper on Lcc gene expression was evaluated by reverse transcriptase polymerase chain reaction.Molecular approaches allowed the detection of Lcc isozymes and suggest the presence of at least two undescribed putative genes.Additionally, Lcc sequences from the both fungal strains clustered with other Lcc sequences from other fungi that inhabit marine environments.

View Article: PubMed Central - PubMed

Affiliation: Divisão de Recursos Microbianos, CPQBA/UNICAMP, CP 6171, 13083-970 Campinas, SP Brazil.

ABSTRACT
The capability of the fungi Nigrospora sp. CBMAI 1328 and Arthopyrenia sp. CBMAI 1330 isolated from marine sponge to synthesise laccases (Lcc) in the presence of the inducer copper (1-10 μM) was assessed. In a liquid culture medium supplemented with 5 μM of copper sulphate after 5 days of incubation, Nigrospora sp. presented the highest Lcc activity (25.2 U·L(-1)). The effect of copper on Lcc gene expression was evaluated by reverse transcriptase polymerase chain reaction. Nigrospora sp. showed the highest gene expression of Lcc under the same conditions of Lcc synthesis. The highest Lcc expression by the Arthopyrenia sp. was detected at 96 h of incubation in absence of copper. Molecular approaches allowed the detection of Lcc isozymes and suggest the presence of at least two undescribed putative genes. Additionally, Lcc sequences from the both fungal strains clustered with other Lcc sequences from other fungi that inhabit marine environments.

No MeSH data available.


Related in: MedlinePlus