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The effect of silver nanoparticles (AgNPs) on proliferation and apoptosis of in ovo cultured glioblastoma multiforme (GBM) cells.

Urbańska K, Pająk B, Orzechowski A, Sokołowska J, Grodzik M, Sawosz E, Szmidt M, Sysa P - Nanoscale Res Lett (2015)

Bottom Line: The results show that AgNPs can influence GBM growth.Although there were statistically significant differences between control and AgNP groups in the AI and the levels of active caspase 9 and active caspase 3, the level of these proteins in GBM cells treated with AgNPs seems to be on the border between the spontaneous apoptosis and the induced.Our results indicate that the antiproliferative properties of silver nanoparticles overwhelm proapoptotic ones.

View Article: PubMed Central - PubMed

Affiliation: Division of Histology and Embryology, Department of Morphological Sciences, Faculty of Veterinary Medicine, Warsaw University of Life Sciences-SGGW, Nowoursynowska 159, 02-776 Warsaw, Poland.

ABSTRACT
Recently, it has been shown that silver nanoparticles (AgNPs) provide a unique approach to the treatment of tumors, especially those of neuroepithelial origin. Thus, the aim of this study was to evaluate the impact of AgNPs on proliferation and activation of the intrinsic apoptotic pathway of glioblastoma multiforme (GBM) cells cultured in an in ovo model. Human GBM cells, line U-87, were placed on chicken embryo chorioallantoic membrane. After 8 days, the tumors were divided into three groups: control (non-treated), treated with colloidal AgNPs (40 μg/ml), and placebo (tumors supplemented with vehicle only). At the end of the experiment, all tumors were isolated. Assessment of cell proliferation and cell apoptosis was estimated by histological, immunohistochemical, and Western blot analyses. The results show that AgNPs can influence GBM growth. AgNPs inhibit proliferation of GBM cells and seem to have proapoptotic properties. Although there were statistically significant differences between control and AgNP groups in the AI and the levels of active caspase 9 and active caspase 3, the level of these proteins in GBM cells treated with AgNPs seems to be on the border between the spontaneous apoptosis and the induced. Our results indicate that the antiproliferative properties of silver nanoparticles overwhelm proapoptotic ones. Further research focused on the cytotoxic effect of AgNPs on tumor and normal cells should be conducted.

No MeSH data available.


Related in: MedlinePlus

Comparing mean values of all immunohistochemically/histologically examined parameters in tumors from control, placebo, and AgNPs groups. Letters (a, b) mean highly statistically significant differences, P ≤ 0.001.
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Fig3: Comparing mean values of all immunohistochemically/histologically examined parameters in tumors from control, placebo, and AgNPs groups. Letters (a, b) mean highly statistically significant differences, P ≤ 0.001.

Mentions: Examination of GBM cells revealed that the PI in control untreated tumors ranged from 21.00 to 36.00% with a mean of 28.72% ± 0.85% (Figure 2A), which was in agreement with the results obtained for the placebo group. In the placebo group, Ki-67+ cells ranged from 21.40 to 35.70% (with a mean of 27.29% ± 0.89%). In the AgNPs-treated group, the PI index was significantly lower (P ≤ 0.001) compared to the control and placebo groups and ranged from 13.70 to 31.00% (mean 20.93% ± 0.69%) (Figure 2B). In the control group, MI ranged from 4.10 to 12.20 (mean 8.54 ± 0.48). In the placebo group, the percentage of GBM cells in metaphase and anaphase was 5.25 to 11.23 (mean 7.89 ± 0.36), comparable to the results for the tumors from the control group. The MI of tumors from the AgNPs group ranged from 1.90 to 10.83 (mean 5.62 ± 0.43). All changes caused by AgNPs administration led to a significant reduction of GBM cells in the M phase compared with cells from control and placebo groups (P ≤ 0.001). Results of MI and PI mean values of all groups are summarized in Figure 3.Figure 2


The effect of silver nanoparticles (AgNPs) on proliferation and apoptosis of in ovo cultured glioblastoma multiforme (GBM) cells.

Urbańska K, Pająk B, Orzechowski A, Sokołowska J, Grodzik M, Sawosz E, Szmidt M, Sysa P - Nanoscale Res Lett (2015)

Comparing mean values of all immunohistochemically/histologically examined parameters in tumors from control, placebo, and AgNPs groups. Letters (a, b) mean highly statistically significant differences, P ≤ 0.001.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4385140&req=5

Fig3: Comparing mean values of all immunohistochemically/histologically examined parameters in tumors from control, placebo, and AgNPs groups. Letters (a, b) mean highly statistically significant differences, P ≤ 0.001.
Mentions: Examination of GBM cells revealed that the PI in control untreated tumors ranged from 21.00 to 36.00% with a mean of 28.72% ± 0.85% (Figure 2A), which was in agreement with the results obtained for the placebo group. In the placebo group, Ki-67+ cells ranged from 21.40 to 35.70% (with a mean of 27.29% ± 0.89%). In the AgNPs-treated group, the PI index was significantly lower (P ≤ 0.001) compared to the control and placebo groups and ranged from 13.70 to 31.00% (mean 20.93% ± 0.69%) (Figure 2B). In the control group, MI ranged from 4.10 to 12.20 (mean 8.54 ± 0.48). In the placebo group, the percentage of GBM cells in metaphase and anaphase was 5.25 to 11.23 (mean 7.89 ± 0.36), comparable to the results for the tumors from the control group. The MI of tumors from the AgNPs group ranged from 1.90 to 10.83 (mean 5.62 ± 0.43). All changes caused by AgNPs administration led to a significant reduction of GBM cells in the M phase compared with cells from control and placebo groups (P ≤ 0.001). Results of MI and PI mean values of all groups are summarized in Figure 3.Figure 2

Bottom Line: The results show that AgNPs can influence GBM growth.Although there were statistically significant differences between control and AgNP groups in the AI and the levels of active caspase 9 and active caspase 3, the level of these proteins in GBM cells treated with AgNPs seems to be on the border between the spontaneous apoptosis and the induced.Our results indicate that the antiproliferative properties of silver nanoparticles overwhelm proapoptotic ones.

View Article: PubMed Central - PubMed

Affiliation: Division of Histology and Embryology, Department of Morphological Sciences, Faculty of Veterinary Medicine, Warsaw University of Life Sciences-SGGW, Nowoursynowska 159, 02-776 Warsaw, Poland.

ABSTRACT
Recently, it has been shown that silver nanoparticles (AgNPs) provide a unique approach to the treatment of tumors, especially those of neuroepithelial origin. Thus, the aim of this study was to evaluate the impact of AgNPs on proliferation and activation of the intrinsic apoptotic pathway of glioblastoma multiforme (GBM) cells cultured in an in ovo model. Human GBM cells, line U-87, were placed on chicken embryo chorioallantoic membrane. After 8 days, the tumors were divided into three groups: control (non-treated), treated with colloidal AgNPs (40 μg/ml), and placebo (tumors supplemented with vehicle only). At the end of the experiment, all tumors were isolated. Assessment of cell proliferation and cell apoptosis was estimated by histological, immunohistochemical, and Western blot analyses. The results show that AgNPs can influence GBM growth. AgNPs inhibit proliferation of GBM cells and seem to have proapoptotic properties. Although there were statistically significant differences between control and AgNP groups in the AI and the levels of active caspase 9 and active caspase 3, the level of these proteins in GBM cells treated with AgNPs seems to be on the border between the spontaneous apoptosis and the induced. Our results indicate that the antiproliferative properties of silver nanoparticles overwhelm proapoptotic ones. Further research focused on the cytotoxic effect of AgNPs on tumor and normal cells should be conducted.

No MeSH data available.


Related in: MedlinePlus