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The effect of silver nanoparticles (AgNPs) on proliferation and apoptosis of in ovo cultured glioblastoma multiforme (GBM) cells.

Urbańska K, Pająk B, Orzechowski A, Sokołowska J, Grodzik M, Sawosz E, Szmidt M, Sysa P - Nanoscale Res Lett (2015)

Bottom Line: The results show that AgNPs can influence GBM growth.Although there were statistically significant differences between control and AgNP groups in the AI and the levels of active caspase 9 and active caspase 3, the level of these proteins in GBM cells treated with AgNPs seems to be on the border between the spontaneous apoptosis and the induced.Our results indicate that the antiproliferative properties of silver nanoparticles overwhelm proapoptotic ones.

View Article: PubMed Central - PubMed

Affiliation: Division of Histology and Embryology, Department of Morphological Sciences, Faculty of Veterinary Medicine, Warsaw University of Life Sciences-SGGW, Nowoursynowska 159, 02-776 Warsaw, Poland.

ABSTRACT
Recently, it has been shown that silver nanoparticles (AgNPs) provide a unique approach to the treatment of tumors, especially those of neuroepithelial origin. Thus, the aim of this study was to evaluate the impact of AgNPs on proliferation and activation of the intrinsic apoptotic pathway of glioblastoma multiforme (GBM) cells cultured in an in ovo model. Human GBM cells, line U-87, were placed on chicken embryo chorioallantoic membrane. After 8 days, the tumors were divided into three groups: control (non-treated), treated with colloidal AgNPs (40 μg/ml), and placebo (tumors supplemented with vehicle only). At the end of the experiment, all tumors were isolated. Assessment of cell proliferation and cell apoptosis was estimated by histological, immunohistochemical, and Western blot analyses. The results show that AgNPs can influence GBM growth. AgNPs inhibit proliferation of GBM cells and seem to have proapoptotic properties. Although there were statistically significant differences between control and AgNP groups in the AI and the levels of active caspase 9 and active caspase 3, the level of these proteins in GBM cells treated with AgNPs seems to be on the border between the spontaneous apoptosis and the induced. Our results indicate that the antiproliferative properties of silver nanoparticles overwhelm proapoptotic ones. Further research focused on the cytotoxic effect of AgNPs on tumor and normal cells should be conducted.

No MeSH data available.


Related in: MedlinePlus

Size distribution and TEM image of silver nanoparticles.
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Fig1: Size distribution and TEM image of silver nanoparticles.

Mentions: The hydrocolloid of nano-Ag (AgNPs) obtained from Nano-Tech (Warsaw, Poland) was produced by an electric non-explosive patented method (patent number US2009020364 A1) from high-purity metals (99.9999%) and high-purity demineralized water [7]. The physical and chemical properties of AgNPs were characterized by Chwalibog et al. [8]. The shape and size of NPs were inspected with a Jeol JEM-1220 transmission electron microscope (TEM) at 80 KeV (JEOL, Tokyo, Japan), with a Morada 11 megapixel camera (Olympus Soft Imaging Solutions GmbH, Münster, Germany) (Figure 1). Samples of Ag for TEM were prepared by placing droplets of hydrocolloids onto formvar-coated copper grids (Agar Scientific Ltd, Stansted, UK). Nanoparticles of Ag were mostly spherical and polydispersed. The stability of the colloidal dispersions of the nanoparticles (zeta potential) was measured by the electrophoretic light-scattering method with a Zetasizer Nano ZS, model ZEN3500 (Malvern Instruments, Worcestershire, UK). The zeta potential of Ag nanoparticles was −36.4 mV, and the average diameter of particles was 70 nm (Figure 1). AgNPs were dissolved in ultra-pure water (Milli-Q water system, Millipore Corp., Billerica, MA, USA).Figure 1


The effect of silver nanoparticles (AgNPs) on proliferation and apoptosis of in ovo cultured glioblastoma multiforme (GBM) cells.

Urbańska K, Pająk B, Orzechowski A, Sokołowska J, Grodzik M, Sawosz E, Szmidt M, Sysa P - Nanoscale Res Lett (2015)

Size distribution and TEM image of silver nanoparticles.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4385140&req=5

Fig1: Size distribution and TEM image of silver nanoparticles.
Mentions: The hydrocolloid of nano-Ag (AgNPs) obtained from Nano-Tech (Warsaw, Poland) was produced by an electric non-explosive patented method (patent number US2009020364 A1) from high-purity metals (99.9999%) and high-purity demineralized water [7]. The physical and chemical properties of AgNPs were characterized by Chwalibog et al. [8]. The shape and size of NPs were inspected with a Jeol JEM-1220 transmission electron microscope (TEM) at 80 KeV (JEOL, Tokyo, Japan), with a Morada 11 megapixel camera (Olympus Soft Imaging Solutions GmbH, Münster, Germany) (Figure 1). Samples of Ag for TEM were prepared by placing droplets of hydrocolloids onto formvar-coated copper grids (Agar Scientific Ltd, Stansted, UK). Nanoparticles of Ag were mostly spherical and polydispersed. The stability of the colloidal dispersions of the nanoparticles (zeta potential) was measured by the electrophoretic light-scattering method with a Zetasizer Nano ZS, model ZEN3500 (Malvern Instruments, Worcestershire, UK). The zeta potential of Ag nanoparticles was −36.4 mV, and the average diameter of particles was 70 nm (Figure 1). AgNPs were dissolved in ultra-pure water (Milli-Q water system, Millipore Corp., Billerica, MA, USA).Figure 1

Bottom Line: The results show that AgNPs can influence GBM growth.Although there were statistically significant differences between control and AgNP groups in the AI and the levels of active caspase 9 and active caspase 3, the level of these proteins in GBM cells treated with AgNPs seems to be on the border between the spontaneous apoptosis and the induced.Our results indicate that the antiproliferative properties of silver nanoparticles overwhelm proapoptotic ones.

View Article: PubMed Central - PubMed

Affiliation: Division of Histology and Embryology, Department of Morphological Sciences, Faculty of Veterinary Medicine, Warsaw University of Life Sciences-SGGW, Nowoursynowska 159, 02-776 Warsaw, Poland.

ABSTRACT
Recently, it has been shown that silver nanoparticles (AgNPs) provide a unique approach to the treatment of tumors, especially those of neuroepithelial origin. Thus, the aim of this study was to evaluate the impact of AgNPs on proliferation and activation of the intrinsic apoptotic pathway of glioblastoma multiforme (GBM) cells cultured in an in ovo model. Human GBM cells, line U-87, were placed on chicken embryo chorioallantoic membrane. After 8 days, the tumors were divided into three groups: control (non-treated), treated with colloidal AgNPs (40 μg/ml), and placebo (tumors supplemented with vehicle only). At the end of the experiment, all tumors were isolated. Assessment of cell proliferation and cell apoptosis was estimated by histological, immunohistochemical, and Western blot analyses. The results show that AgNPs can influence GBM growth. AgNPs inhibit proliferation of GBM cells and seem to have proapoptotic properties. Although there were statistically significant differences between control and AgNP groups in the AI and the levels of active caspase 9 and active caspase 3, the level of these proteins in GBM cells treated with AgNPs seems to be on the border between the spontaneous apoptosis and the induced. Our results indicate that the antiproliferative properties of silver nanoparticles overwhelm proapoptotic ones. Further research focused on the cytotoxic effect of AgNPs on tumor and normal cells should be conducted.

No MeSH data available.


Related in: MedlinePlus