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Composites of silica with immobilized cholinesterase incorporated into polymeric shell.

Payentko V, Matkovsky A, Matrunchik Y - Nanoscale Res Lett (2015)

Bottom Line: Evaluation of the cholinesterase activity has been fulfilled through the imitation of the acetylcholine chloride decomposition reaction.Values of activity for cholinesterase nanocomposites demonstrated in this work are higher than those for the native cholinesterase.The higher activity of cholinesterase contained in nanocomposites was found for those prepared using highly dispersed silica.

View Article: PubMed Central - PubMed

Affiliation: Department of Amorphous and Structurally Ordered Oxides, Chuiko Institute of Surface Chemistry, National Academy of Sciences of Ukraine, 17, General Naumov str., Kyiv, 03164 Ukraine.

ABSTRACT
Synthetic approaches for new nanocomposite materials with relatively high cholinesterase activity have been developed. The peculiarity of the formation of such systems is the introduction of cholinesterase into polymer with subsequent incorporation on the ready-made silica particles and into the polysiloxane matrixes during sol-gel synthesis. Evaluation of the cholinesterase activity has been fulfilled through the imitation of the acetylcholine chloride decomposition reaction. Values of activity for cholinesterase nanocomposites demonstrated in this work are higher than those for the native cholinesterase. The higher activity of cholinesterase contained in nanocomposites was found for those prepared using highly dispersed silica.

No MeSH data available.


Microphotographs of two types of hybrid materials obtained using scanning electron microscopy (SEM). Formation of silica components in composites (A) through the sol-gel process and (B) by means of A300 particle aggregation.
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Fig2: Microphotographs of two types of hybrid materials obtained using scanning electron microscopy (SEM). Formation of silica components in composites (A) through the sol-gel process and (B) by means of A300 particle aggregation.

Mentions: Enzyme specimen in immobilized state demonstrates higher values of catalytic activity compared to native forms (Table 1). Utilizing highly dispersed silica (A300) as silica component in composite allows retaining the higher values of catalytic activity in the reaction of acetylcholine chloride hydrolysis. This may be explained by strong difference in morphology of composites obtained by two procedures which may be seen from SEM microphotographs (Figure 2).Figure 2


Composites of silica with immobilized cholinesterase incorporated into polymeric shell.

Payentko V, Matkovsky A, Matrunchik Y - Nanoscale Res Lett (2015)

Microphotographs of two types of hybrid materials obtained using scanning electron microscopy (SEM). Formation of silica components in composites (A) through the sol-gel process and (B) by means of A300 particle aggregation.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4385111&req=5

Fig2: Microphotographs of two types of hybrid materials obtained using scanning electron microscopy (SEM). Formation of silica components in composites (A) through the sol-gel process and (B) by means of A300 particle aggregation.
Mentions: Enzyme specimen in immobilized state demonstrates higher values of catalytic activity compared to native forms (Table 1). Utilizing highly dispersed silica (A300) as silica component in composite allows retaining the higher values of catalytic activity in the reaction of acetylcholine chloride hydrolysis. This may be explained by strong difference in morphology of composites obtained by two procedures which may be seen from SEM microphotographs (Figure 2).Figure 2

Bottom Line: Evaluation of the cholinesterase activity has been fulfilled through the imitation of the acetylcholine chloride decomposition reaction.Values of activity for cholinesterase nanocomposites demonstrated in this work are higher than those for the native cholinesterase.The higher activity of cholinesterase contained in nanocomposites was found for those prepared using highly dispersed silica.

View Article: PubMed Central - PubMed

Affiliation: Department of Amorphous and Structurally Ordered Oxides, Chuiko Institute of Surface Chemistry, National Academy of Sciences of Ukraine, 17, General Naumov str., Kyiv, 03164 Ukraine.

ABSTRACT
Synthetic approaches for new nanocomposite materials with relatively high cholinesterase activity have been developed. The peculiarity of the formation of such systems is the introduction of cholinesterase into polymer with subsequent incorporation on the ready-made silica particles and into the polysiloxane matrixes during sol-gel synthesis. Evaluation of the cholinesterase activity has been fulfilled through the imitation of the acetylcholine chloride decomposition reaction. Values of activity for cholinesterase nanocomposites demonstrated in this work are higher than those for the native cholinesterase. The higher activity of cholinesterase contained in nanocomposites was found for those prepared using highly dispersed silica.

No MeSH data available.