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Composites of silica with immobilized cholinesterase incorporated into polymeric shell.

Payentko V, Matkovsky A, Matrunchik Y - Nanoscale Res Lett (2015)

Bottom Line: Evaluation of the cholinesterase activity has been fulfilled through the imitation of the acetylcholine chloride decomposition reaction.Values of activity for cholinesterase nanocomposites demonstrated in this work are higher than those for the native cholinesterase.The higher activity of cholinesterase contained in nanocomposites was found for those prepared using highly dispersed silica.

View Article: PubMed Central - PubMed

Affiliation: Department of Amorphous and Structurally Ordered Oxides, Chuiko Institute of Surface Chemistry, National Academy of Sciences of Ukraine, 17, General Naumov str., Kyiv, 03164 Ukraine.

ABSTRACT
Synthetic approaches for new nanocomposite materials with relatively high cholinesterase activity have been developed. The peculiarity of the formation of such systems is the introduction of cholinesterase into polymer with subsequent incorporation on the ready-made silica particles and into the polysiloxane matrixes during sol-gel synthesis. Evaluation of the cholinesterase activity has been fulfilled through the imitation of the acetylcholine chloride decomposition reaction. Values of activity for cholinesterase nanocomposites demonstrated in this work are higher than those for the native cholinesterase. The higher activity of cholinesterase contained in nanocomposites was found for those prepared using highly dispersed silica.

No MeSH data available.


Hydrolysis of acetylcholine using acetylcholinesterase.
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Fig1: Hydrolysis of acetylcholine using acetylcholinesterase.

Mentions: To evaluate the cholinesterase activity, a trimetric method [9] was employed according to Figure 1. Nanocomposite samples were placed in a water bath maintained at 38°C and the pH adjusted to 7.4 with 0.1 N NaOH; the acetylcholine was then added to the mixture.Figure 1


Composites of silica with immobilized cholinesterase incorporated into polymeric shell.

Payentko V, Matkovsky A, Matrunchik Y - Nanoscale Res Lett (2015)

Hydrolysis of acetylcholine using acetylcholinesterase.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4385111&req=5

Fig1: Hydrolysis of acetylcholine using acetylcholinesterase.
Mentions: To evaluate the cholinesterase activity, a trimetric method [9] was employed according to Figure 1. Nanocomposite samples were placed in a water bath maintained at 38°C and the pH adjusted to 7.4 with 0.1 N NaOH; the acetylcholine was then added to the mixture.Figure 1

Bottom Line: Evaluation of the cholinesterase activity has been fulfilled through the imitation of the acetylcholine chloride decomposition reaction.Values of activity for cholinesterase nanocomposites demonstrated in this work are higher than those for the native cholinesterase.The higher activity of cholinesterase contained in nanocomposites was found for those prepared using highly dispersed silica.

View Article: PubMed Central - PubMed

Affiliation: Department of Amorphous and Structurally Ordered Oxides, Chuiko Institute of Surface Chemistry, National Academy of Sciences of Ukraine, 17, General Naumov str., Kyiv, 03164 Ukraine.

ABSTRACT
Synthetic approaches for new nanocomposite materials with relatively high cholinesterase activity have been developed. The peculiarity of the formation of such systems is the introduction of cholinesterase into polymer with subsequent incorporation on the ready-made silica particles and into the polysiloxane matrixes during sol-gel synthesis. Evaluation of the cholinesterase activity has been fulfilled through the imitation of the acetylcholine chloride decomposition reaction. Values of activity for cholinesterase nanocomposites demonstrated in this work are higher than those for the native cholinesterase. The higher activity of cholinesterase contained in nanocomposites was found for those prepared using highly dispersed silica.

No MeSH data available.