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Nano- and microsized zeolites as a perspective material for potentiometric biosensors creation.

Soldatkin OO, Shelyakina MK, Arkhypova VN, Soy E, Kirdeciler SK, Ozansoy Kasap B, Lagarde F, Jaffrezic-Renault N, Akata Kurç B, Soldatkin AP, Dzyadevych SV - Nanoscale Res Lett (2015)

Bottom Line: A number of potentiometric biosensors based on coimmobilization of enzymes with different types of zeolite on pH-ion-sensitive field-effect transistor (ISFET) have been developed.Their working characteristics have been determined and compared.These results show that it is possible to regulate the ISFET characteristics for different enzyme-based biosensors by tailoring the electrode surfaces via different zeolites.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Biomolecular Electronics, Institute of Molecular Biology and Genetics, National Academy of Sciences of Ukraine, Zabolotnogo Street 150, 03143 Kyiv, Ukraine ; Taras Shevchenko National University of Kyiv, Volodymyrska Street 64, 01601 Kyiv, Ukraine.

ABSTRACT
A number of potentiometric biosensors based on coimmobilization of enzymes with different types of zeolite on pH-ion-sensitive field-effect transistor (ISFET) have been developed. Their working characteristics have been determined and compared. It was shown that clinoptilolite and zeolite Beta polymorph A (BEA) are more promising for creating biosensors than zeolite A. Changing the concentration of zeolite BEA in membranes, it is possible to extend the biosensor linear measurement range. The two-layer method of deposition of the enzyme with clinoptilolite was found to provide a significant increase in the biosensor sensitivity to substrates, whereas thermal modification of the zeolite BEA crystals can improve analytical characteristics of potentiometric biosensors for detection of toxic substances. These results show that it is possible to regulate the ISFET characteristics for different enzyme-based biosensors by tailoring the electrode surfaces via different zeolites. This makes zeolites strong candidates for integration into biosensors as ISFET modifiers.

No MeSH data available.


Related in: MedlinePlus

Dependence of residual activity of bio-membranes. They were based on BuChE (a) and urease (b) on concentration of glycoalkaloids and mercury ions (Hg + 2). Measurements were conducted in 5 mM phosphate buffer, pH 7.4. Figure legend: biosensor without zeolite (1) and with different heat-treated zeolite Beta: BEA-1 (1), BEA-2 (2), and BEA-3 (3).
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Fig9: Dependence of residual activity of bio-membranes. They were based on BuChE (a) and urease (b) on concentration of glycoalkaloids and mercury ions (Hg + 2). Measurements were conducted in 5 mM phosphate buffer, pH 7.4. Figure legend: biosensor without zeolite (1) and with different heat-treated zeolite Beta: BEA-1 (1), BEA-2 (2), and BEA-3 (3).

Mentions: The effect of different heat-treated zeolite samples incorporated in bioselective membranes on the biosensor sensitivity to glycoalkaloids and heavy metal ions was investigated using BuChCl and urea biosensors, respectively. The calibration curves of residual activity of biomembranes based on BuChE and urease as a function of the concentration of glycoalkaloids and mercury ions (Hg2+) are presented in Figure 9a,b, respectively. As shown in Figure 9a, all BuChCl biosensors based on zeolite Beta showed higher sensitivity to glycoalkaloids than the zeolite-free biosensor and these sensitivities decreased in the order of BEA-3 > BEA-2 > BEA-1. Similar trends were observed for the mercury ions in urea biosensors (Figure 9b).Figure 9


Nano- and microsized zeolites as a perspective material for potentiometric biosensors creation.

Soldatkin OO, Shelyakina MK, Arkhypova VN, Soy E, Kirdeciler SK, Ozansoy Kasap B, Lagarde F, Jaffrezic-Renault N, Akata Kurç B, Soldatkin AP, Dzyadevych SV - Nanoscale Res Lett (2015)

Dependence of residual activity of bio-membranes. They were based on BuChE (a) and urease (b) on concentration of glycoalkaloids and mercury ions (Hg + 2). Measurements were conducted in 5 mM phosphate buffer, pH 7.4. Figure legend: biosensor without zeolite (1) and with different heat-treated zeolite Beta: BEA-1 (1), BEA-2 (2), and BEA-3 (3).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4385093&req=5

Fig9: Dependence of residual activity of bio-membranes. They were based on BuChE (a) and urease (b) on concentration of glycoalkaloids and mercury ions (Hg + 2). Measurements were conducted in 5 mM phosphate buffer, pH 7.4. Figure legend: biosensor without zeolite (1) and with different heat-treated zeolite Beta: BEA-1 (1), BEA-2 (2), and BEA-3 (3).
Mentions: The effect of different heat-treated zeolite samples incorporated in bioselective membranes on the biosensor sensitivity to glycoalkaloids and heavy metal ions was investigated using BuChCl and urea biosensors, respectively. The calibration curves of residual activity of biomembranes based on BuChE and urease as a function of the concentration of glycoalkaloids and mercury ions (Hg2+) are presented in Figure 9a,b, respectively. As shown in Figure 9a, all BuChCl biosensors based on zeolite Beta showed higher sensitivity to glycoalkaloids than the zeolite-free biosensor and these sensitivities decreased in the order of BEA-3 > BEA-2 > BEA-1. Similar trends were observed for the mercury ions in urea biosensors (Figure 9b).Figure 9

Bottom Line: A number of potentiometric biosensors based on coimmobilization of enzymes with different types of zeolite on pH-ion-sensitive field-effect transistor (ISFET) have been developed.Their working characteristics have been determined and compared.These results show that it is possible to regulate the ISFET characteristics for different enzyme-based biosensors by tailoring the electrode surfaces via different zeolites.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Biomolecular Electronics, Institute of Molecular Biology and Genetics, National Academy of Sciences of Ukraine, Zabolotnogo Street 150, 03143 Kyiv, Ukraine ; Taras Shevchenko National University of Kyiv, Volodymyrska Street 64, 01601 Kyiv, Ukraine.

ABSTRACT
A number of potentiometric biosensors based on coimmobilization of enzymes with different types of zeolite on pH-ion-sensitive field-effect transistor (ISFET) have been developed. Their working characteristics have been determined and compared. It was shown that clinoptilolite and zeolite Beta polymorph A (BEA) are more promising for creating biosensors than zeolite A. Changing the concentration of zeolite BEA in membranes, it is possible to extend the biosensor linear measurement range. The two-layer method of deposition of the enzyme with clinoptilolite was found to provide a significant increase in the biosensor sensitivity to substrates, whereas thermal modification of the zeolite BEA crystals can improve analytical characteristics of potentiometric biosensors for detection of toxic substances. These results show that it is possible to regulate the ISFET characteristics for different enzyme-based biosensors by tailoring the electrode surfaces via different zeolites. This makes zeolites strong candidates for integration into biosensors as ISFET modifiers.

No MeSH data available.


Related in: MedlinePlus