Limits...
Nano- and microsized zeolites as a perspective material for potentiometric biosensors creation.

Soldatkin OO, Shelyakina MK, Arkhypova VN, Soy E, Kirdeciler SK, Ozansoy Kasap B, Lagarde F, Jaffrezic-Renault N, Akata Kurç B, Soldatkin AP, Dzyadevych SV - Nanoscale Res Lett (2015)

Bottom Line: Their working characteristics have been determined and compared.The two-layer method of deposition of the enzyme with clinoptilolite was found to provide a significant increase in the biosensor sensitivity to substrates, whereas thermal modification of the zeolite BEA crystals can improve analytical characteristics of potentiometric biosensors for detection of toxic substances.These results show that it is possible to regulate the ISFET characteristics for different enzyme-based biosensors by tailoring the electrode surfaces via different zeolites.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Biomolecular Electronics, Institute of Molecular Biology and Genetics, National Academy of Sciences of Ukraine, Zabolotnogo Street 150, 03143 Kyiv, Ukraine ; Taras Shevchenko National University of Kyiv, Volodymyrska Street 64, 01601 Kyiv, Ukraine.

ABSTRACT
A number of potentiometric biosensors based on coimmobilization of enzymes with different types of zeolite on pH-ion-sensitive field-effect transistor (ISFET) have been developed. Their working characteristics have been determined and compared. It was shown that clinoptilolite and zeolite Beta polymorph A (BEA) are more promising for creating biosensors than zeolite A. Changing the concentration of zeolite BEA in membranes, it is possible to extend the biosensor linear measurement range. The two-layer method of deposition of the enzyme with clinoptilolite was found to provide a significant increase in the biosensor sensitivity to substrates, whereas thermal modification of the zeolite BEA crystals can improve analytical characteristics of potentiometric biosensors for detection of toxic substances. These results show that it is possible to regulate the ISFET characteristics for different enzyme-based biosensors by tailoring the electrode surfaces via different zeolites. This makes zeolites strong candidates for integration into biosensors as ISFET modifiers.

No MeSH data available.


Calibration curves of different urea biosensors. They were based on: first (1), second (2), and third (3) variant of coimmobilization of urease with zeolite H+-BEA-30. Measurements were conducted in 5 mM phosphate buffer, pH 7.2.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4385093&req=5

Fig5: Calibration curves of different urea biosensors. They were based on: first (1), second (2), and third (3) variant of coimmobilization of urease with zeolite H+-BEA-30. Measurements were conducted in 5 mM phosphate buffer, pH 7.2.

Mentions: An effect of slight increase in the biosensors sensitivity (about 20%) in the presence of zeolite H+-BEA-30 in the enzyme membrane was obtained at simultaneous immobilization of the enzyme together with zeolite in a mixture (one-layer immobilization). To enhance the positive effect, we attempted to perform two-layer immobilization. Three variants of the bioselective element construction were tested. The first one consisted of deposition of the enzyme membrane on the transducer surface (first layer) and the zeolite membrane as the second layer. In the second variant, the layers were placed vice versa. The third variant: first - zeolite layer and next layer - enzyme-zeolite mixture. A number of biosensors obtained by two-layer methods (all three variants) were examined; their calibration curves are shown in Figure 5. As seen, the biosensor with H+-BEA-30 zeolite immobilized according to the third variant had 50% higher activity than that of the biosensors with urease only.Figure 5


Nano- and microsized zeolites as a perspective material for potentiometric biosensors creation.

Soldatkin OO, Shelyakina MK, Arkhypova VN, Soy E, Kirdeciler SK, Ozansoy Kasap B, Lagarde F, Jaffrezic-Renault N, Akata Kurç B, Soldatkin AP, Dzyadevych SV - Nanoscale Res Lett (2015)

Calibration curves of different urea biosensors. They were based on: first (1), second (2), and third (3) variant of coimmobilization of urease with zeolite H+-BEA-30. Measurements were conducted in 5 mM phosphate buffer, pH 7.2.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4385093&req=5

Fig5: Calibration curves of different urea biosensors. They were based on: first (1), second (2), and third (3) variant of coimmobilization of urease with zeolite H+-BEA-30. Measurements were conducted in 5 mM phosphate buffer, pH 7.2.
Mentions: An effect of slight increase in the biosensors sensitivity (about 20%) in the presence of zeolite H+-BEA-30 in the enzyme membrane was obtained at simultaneous immobilization of the enzyme together with zeolite in a mixture (one-layer immobilization). To enhance the positive effect, we attempted to perform two-layer immobilization. Three variants of the bioselective element construction were tested. The first one consisted of deposition of the enzyme membrane on the transducer surface (first layer) and the zeolite membrane as the second layer. In the second variant, the layers were placed vice versa. The third variant: first - zeolite layer and next layer - enzyme-zeolite mixture. A number of biosensors obtained by two-layer methods (all three variants) were examined; their calibration curves are shown in Figure 5. As seen, the biosensor with H+-BEA-30 zeolite immobilized according to the third variant had 50% higher activity than that of the biosensors with urease only.Figure 5

Bottom Line: Their working characteristics have been determined and compared.The two-layer method of deposition of the enzyme with clinoptilolite was found to provide a significant increase in the biosensor sensitivity to substrates, whereas thermal modification of the zeolite BEA crystals can improve analytical characteristics of potentiometric biosensors for detection of toxic substances.These results show that it is possible to regulate the ISFET characteristics for different enzyme-based biosensors by tailoring the electrode surfaces via different zeolites.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Biomolecular Electronics, Institute of Molecular Biology and Genetics, National Academy of Sciences of Ukraine, Zabolotnogo Street 150, 03143 Kyiv, Ukraine ; Taras Shevchenko National University of Kyiv, Volodymyrska Street 64, 01601 Kyiv, Ukraine.

ABSTRACT
A number of potentiometric biosensors based on coimmobilization of enzymes with different types of zeolite on pH-ion-sensitive field-effect transistor (ISFET) have been developed. Their working characteristics have been determined and compared. It was shown that clinoptilolite and zeolite Beta polymorph A (BEA) are more promising for creating biosensors than zeolite A. Changing the concentration of zeolite BEA in membranes, it is possible to extend the biosensor linear measurement range. The two-layer method of deposition of the enzyme with clinoptilolite was found to provide a significant increase in the biosensor sensitivity to substrates, whereas thermal modification of the zeolite BEA crystals can improve analytical characteristics of potentiometric biosensors for detection of toxic substances. These results show that it is possible to regulate the ISFET characteristics for different enzyme-based biosensors by tailoring the electrode surfaces via different zeolites. This makes zeolites strong candidates for integration into biosensors as ISFET modifiers.

No MeSH data available.