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Nano- and microsized zeolites as a perspective material for potentiometric biosensors creation.

Soldatkin OO, Shelyakina MK, Arkhypova VN, Soy E, Kirdeciler SK, Ozansoy Kasap B, Lagarde F, Jaffrezic-Renault N, Akata Kurç B, Soldatkin AP, Dzyadevych SV - Nanoscale Res Lett (2015)

Bottom Line: Their working characteristics have been determined and compared.The two-layer method of deposition of the enzyme with clinoptilolite was found to provide a significant increase in the biosensor sensitivity to substrates, whereas thermal modification of the zeolite BEA crystals can improve analytical characteristics of potentiometric biosensors for detection of toxic substances.These results show that it is possible to regulate the ISFET characteristics for different enzyme-based biosensors by tailoring the electrode surfaces via different zeolites.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Biomolecular Electronics, Institute of Molecular Biology and Genetics, National Academy of Sciences of Ukraine, Zabolotnogo Street 150, 03143 Kyiv, Ukraine ; Taras Shevchenko National University of Kyiv, Volodymyrska Street 64, 01601 Kyiv, Ukraine.

ABSTRACT
A number of potentiometric biosensors based on coimmobilization of enzymes with different types of zeolite on pH-ion-sensitive field-effect transistor (ISFET) have been developed. Their working characteristics have been determined and compared. It was shown that clinoptilolite and zeolite Beta polymorph A (BEA) are more promising for creating biosensors than zeolite A. Changing the concentration of zeolite BEA in membranes, it is possible to extend the biosensor linear measurement range. The two-layer method of deposition of the enzyme with clinoptilolite was found to provide a significant increase in the biosensor sensitivity to substrates, whereas thermal modification of the zeolite BEA crystals can improve analytical characteristics of potentiometric biosensors for detection of toxic substances. These results show that it is possible to regulate the ISFET characteristics for different enzyme-based biosensors by tailoring the electrode surfaces via different zeolites. This makes zeolites strong candidates for integration into biosensors as ISFET modifiers.

No MeSH data available.


Scanning electron microscopy images of synthesized LTA-9 (a) and BEA-40 (b) zeolites.
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Fig1: Scanning electron microscopy images of synthesized LTA-9 (a) and BEA-40 (b) zeolites.

Mentions: The results of scanning electron microscopy images of synthesized LTA-9 and BEA-40 zeolite are presented in Figure 1.Figure 1


Nano- and microsized zeolites as a perspective material for potentiometric biosensors creation.

Soldatkin OO, Shelyakina MK, Arkhypova VN, Soy E, Kirdeciler SK, Ozansoy Kasap B, Lagarde F, Jaffrezic-Renault N, Akata Kurç B, Soldatkin AP, Dzyadevych SV - Nanoscale Res Lett (2015)

Scanning electron microscopy images of synthesized LTA-9 (a) and BEA-40 (b) zeolites.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4385093&req=5

Fig1: Scanning electron microscopy images of synthesized LTA-9 (a) and BEA-40 (b) zeolites.
Mentions: The results of scanning electron microscopy images of synthesized LTA-9 and BEA-40 zeolite are presented in Figure 1.Figure 1

Bottom Line: Their working characteristics have been determined and compared.The two-layer method of deposition of the enzyme with clinoptilolite was found to provide a significant increase in the biosensor sensitivity to substrates, whereas thermal modification of the zeolite BEA crystals can improve analytical characteristics of potentiometric biosensors for detection of toxic substances.These results show that it is possible to regulate the ISFET characteristics for different enzyme-based biosensors by tailoring the electrode surfaces via different zeolites.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Biomolecular Electronics, Institute of Molecular Biology and Genetics, National Academy of Sciences of Ukraine, Zabolotnogo Street 150, 03143 Kyiv, Ukraine ; Taras Shevchenko National University of Kyiv, Volodymyrska Street 64, 01601 Kyiv, Ukraine.

ABSTRACT
A number of potentiometric biosensors based on coimmobilization of enzymes with different types of zeolite on pH-ion-sensitive field-effect transistor (ISFET) have been developed. Their working characteristics have been determined and compared. It was shown that clinoptilolite and zeolite Beta polymorph A (BEA) are more promising for creating biosensors than zeolite A. Changing the concentration of zeolite BEA in membranes, it is possible to extend the biosensor linear measurement range. The two-layer method of deposition of the enzyme with clinoptilolite was found to provide a significant increase in the biosensor sensitivity to substrates, whereas thermal modification of the zeolite BEA crystals can improve analytical characteristics of potentiometric biosensors for detection of toxic substances. These results show that it is possible to regulate the ISFET characteristics for different enzyme-based biosensors by tailoring the electrode surfaces via different zeolites. This makes zeolites strong candidates for integration into biosensors as ISFET modifiers.

No MeSH data available.