Limits...
Biomarkers of fatigue related to adjuvant chemotherapy for breast cancer: evaluation of plasma and lymphocyte expression.

Cruz FM, Munhoz BA, Alves BC, Gehrke FS, Fonseca FL, Kuniyoshi RK, Cubero D, Peppone LJ, Del Giglio A - Clin Transl Med (2015)

Bottom Line: We evaluated FSH, LH, estradiol, DHEA, DHEAS, IL6, IL2, ILIRA, IL1β, CRP, Cortisol in the plasma and IL2, IL10, IL6, TGF-β, KLRC1, TNF, BTP, SNCA, SOD1, BLNK, PTGS2 and INF γ expression in the PMNCF. 11 patients did not exhibit an increase in their BFI scores and served as controls, whereas 32 patients exhibited an increase in their BFI scores compared with the baseline scores.We conclude that fatigue induced by chemotherapy in BC patients is associated with changes in IL1-ra plasma levels and in TGF-β lymphocyte expression.Its mechanism may be different than that observed in long-term BC survivors or that induced by radiation therapy.

View Article: PubMed Central - PubMed

Affiliation: Discipline of Oncology and Hematology, ABC Foundation School of Medicine, Av. Príncipe de Gales, 821, Santo André, 09060-650 SP Brazil.

ABSTRACT

Background: Fatigue is common in cancer patients receiving adjuvant chemotherapy. To further understand the mechanism of fatigue and search for potential biomarkers, we conducted this prospective study. Methods We enrolled breast cancer (BC) patients before their first adjuvant Adriamycin-based chemotherapy cycle. Patients responded to the brief fatigue inventory (BFI) and Chalder fatigue questionnaires and had their blood drawn for both plasma evaluation and evaluation of the peripheral mononuclear cell fraction (PMNCF) mRNA expression of various biomarkers. We evaluated FSH, LH, estradiol, DHEA, DHEAS, IL6, IL2, ILIRA, IL1β, CRP, Cortisol in the plasma and IL2, IL10, IL6, TGF-β, KLRC1, TNF, BTP, SNCA, SOD1, BLNK, PTGS2 and INF γ expression in the PMNCF.

Results: 11 patients did not exhibit an increase in their BFI scores and served as controls, whereas 32 patients exhibited an increase in their BFI scores compared with the baseline scores. From the biomarkers we evaluated in the PMNCF, the only one significantly associated with fatigue was TGF-β (p = 0.0343), while there was a trend towards significance with KLRC1 (p = 0.0627). We observed no evidence of significant associations of any plasma biomarkers with the development of fatigue. However when we analyzed patients with more severe fatigue, plasma IL1-RA levels correlated directly with higher fatigue scores (p = 0.0136).

Conclusions: We conclude that fatigue induced by chemotherapy in BC patients is associated with changes in IL1-ra plasma levels and in TGF-β lymphocyte expression. Its mechanism may be different than that observed in long-term BC survivors or that induced by radiation therapy.

Trial registration: NCT02041364 [ClinicalTrials.gov].

No MeSH data available.


Related in: MedlinePlus

Evaluation of lymphocyte expression of selected genes in the first phase of the study in patients in the inclusion phase whose fatigue deteriorated and in patients whose fatigue did not worsen after the first cycle of chemotherapy (controls). The black dots represent the outliers.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4385032&req=5

Fig3: Evaluation of lymphocyte expression of selected genes in the first phase of the study in patients in the inclusion phase whose fatigue deteriorated and in patients whose fatigue did not worsen after the first cycle of chemotherapy (controls). The black dots represent the outliers.

Mentions: Figure 1 reveals the significant variations of fatigue in each group being evaluated in Figures 2 and 3, which in turn illustrate the changes observed in the biomarkers evaluated in the blood and lymphocytes, respectively, for all the study patients.Figure 1


Biomarkers of fatigue related to adjuvant chemotherapy for breast cancer: evaluation of plasma and lymphocyte expression.

Cruz FM, Munhoz BA, Alves BC, Gehrke FS, Fonseca FL, Kuniyoshi RK, Cubero D, Peppone LJ, Del Giglio A - Clin Transl Med (2015)

Evaluation of lymphocyte expression of selected genes in the first phase of the study in patients in the inclusion phase whose fatigue deteriorated and in patients whose fatigue did not worsen after the first cycle of chemotherapy (controls). The black dots represent the outliers.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4385032&req=5

Fig3: Evaluation of lymphocyte expression of selected genes in the first phase of the study in patients in the inclusion phase whose fatigue deteriorated and in patients whose fatigue did not worsen after the first cycle of chemotherapy (controls). The black dots represent the outliers.
Mentions: Figure 1 reveals the significant variations of fatigue in each group being evaluated in Figures 2 and 3, which in turn illustrate the changes observed in the biomarkers evaluated in the blood and lymphocytes, respectively, for all the study patients.Figure 1

Bottom Line: We evaluated FSH, LH, estradiol, DHEA, DHEAS, IL6, IL2, ILIRA, IL1β, CRP, Cortisol in the plasma and IL2, IL10, IL6, TGF-β, KLRC1, TNF, BTP, SNCA, SOD1, BLNK, PTGS2 and INF γ expression in the PMNCF. 11 patients did not exhibit an increase in their BFI scores and served as controls, whereas 32 patients exhibited an increase in their BFI scores compared with the baseline scores.We conclude that fatigue induced by chemotherapy in BC patients is associated with changes in IL1-ra plasma levels and in TGF-β lymphocyte expression.Its mechanism may be different than that observed in long-term BC survivors or that induced by radiation therapy.

View Article: PubMed Central - PubMed

Affiliation: Discipline of Oncology and Hematology, ABC Foundation School of Medicine, Av. Príncipe de Gales, 821, Santo André, 09060-650 SP Brazil.

ABSTRACT

Background: Fatigue is common in cancer patients receiving adjuvant chemotherapy. To further understand the mechanism of fatigue and search for potential biomarkers, we conducted this prospective study. Methods We enrolled breast cancer (BC) patients before their first adjuvant Adriamycin-based chemotherapy cycle. Patients responded to the brief fatigue inventory (BFI) and Chalder fatigue questionnaires and had their blood drawn for both plasma evaluation and evaluation of the peripheral mononuclear cell fraction (PMNCF) mRNA expression of various biomarkers. We evaluated FSH, LH, estradiol, DHEA, DHEAS, IL6, IL2, ILIRA, IL1β, CRP, Cortisol in the plasma and IL2, IL10, IL6, TGF-β, KLRC1, TNF, BTP, SNCA, SOD1, BLNK, PTGS2 and INF γ expression in the PMNCF.

Results: 11 patients did not exhibit an increase in their BFI scores and served as controls, whereas 32 patients exhibited an increase in their BFI scores compared with the baseline scores. From the biomarkers we evaluated in the PMNCF, the only one significantly associated with fatigue was TGF-β (p = 0.0343), while there was a trend towards significance with KLRC1 (p = 0.0627). We observed no evidence of significant associations of any plasma biomarkers with the development of fatigue. However when we analyzed patients with more severe fatigue, plasma IL1-RA levels correlated directly with higher fatigue scores (p = 0.0136).

Conclusions: We conclude that fatigue induced by chemotherapy in BC patients is associated with changes in IL1-ra plasma levels and in TGF-β lymphocyte expression. Its mechanism may be different than that observed in long-term BC survivors or that induced by radiation therapy.

Trial registration: NCT02041364 [ClinicalTrials.gov].

No MeSH data available.


Related in: MedlinePlus