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Microbial production of Propionic and Succinic acid from Sorbitol using Propionibacterium acidipropionici.

Duarte JC, Valença GP, Moran PJ, Rodrigues JA - AMB Express (2015)

Bottom Line: The concentration of propionic acid decreased slightly from 39.5 ± 5.2 g L(-1) to 34.4 ± 1.9 g L(-1), and that of succinic acid increased significantly from 6.1 ± 2.1 g L(-1) to 14.8 ± 0.9 g L(-1) through the sequential batches.In addition, a small amount of acetic acid was produced that decreased from 3.3 ± 0.4 g L(-1) to 2.0 ± 0.3 g L(-1) through the batches.The major yield for propionic acid was 0.613 g g(-1) in the first batch and succinic acid it was 0.212 g g(-1) in the third batch.

View Article: PubMed Central - PubMed

Affiliation: Institute of Chemistry, University of Campinas, Campinas, SP 13083-970 Brazil ; School of Chemical Engineering, University of Campinas, Campinas, SP 13083-852 Brazil.

ABSTRACT
Three sequential fermentative batches were carried out with cell recycle in four simultaneously operating bioreactors maintained at pH 6.5, 30°C, and 100 rpm. P. acidipropionici ATCC 4875 was able to produce propionic and succinic acid from sorbitol. The concentration of propionic acid decreased slightly from 39.5 ± 5.2 g L(-1) to 34.4 ± 1.9 g L(-1), and that of succinic acid increased significantly from 6.1 ± 2.1 g L(-1) to 14.8 ± 0.9 g L(-1) through the sequential batches. In addition, a small amount of acetic acid was produced that decreased from 3.3 ± 0.4 g L(-1) to 2.0 ± 0.3 g L(-1) through the batches. The major yield for propionic acid was 0.613 g g(-1) in the first batch and succinic acid it was 0.212 g g(-1) in the third batch. The minor yield of acetic acid was 0.029 g g(-1), in the second and third batches.

No MeSH data available.


Related in: MedlinePlus

Profile of sorbitol fermentation byP. acidipropionicifor the first batch operating in four independent vessels simultaneously.
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Fig1: Profile of sorbitol fermentation byP. acidipropionicifor the first batch operating in four independent vessels simultaneously.

Mentions: Three sequential batches in two independent bioreactors, each one with two parallel vessels (four reactors) operating simultaneously, were carried out with P. acidipropionici ATCC 4875 using sorbitol as a carbon source. The results for the first batch are presented in Figure 1. It was observed that sorbitol was not completely consumed after 70 h, and its final concentration was 13.3 ± 0.9 g L−1. The final concentrations of succinic, acetic, and propionic acid were 6.1 ± 2.1, 3.3 ± 0.4, and 39.5 ± 5.2 g L−1, respectively. Cell growth was also observed, and the final cellular concentration was 4.3 ± 0.1 g L−1. In the second sequential batch, contrarily to the first batch, the substrate consumption was almost complete after 70 h, and its final concentration was 1.2 ± 1.3 g L−1. The final concentrations of succinic, acetic, and propionic acid were 10.0 ± 1.3, 2.2 ± 0.1, and 35.8 ± 1.4 g L−1, respectively. The final concentration of succinic acid increased while that of acetic and propionic acid decreased, compared with the first batch. As expected, cell growth was observed, and the final cellular concentration increased up to 6.1 ± 0.3 g L−1 (Figure 2). In the last sequential batch, substrate consumption was completed before 70 h, and the final concentrations of succinic, acetic, and propionic acid were 14.8 ± 0.9, 2.0 ± 0.3, and 34.4 ± 1.9 g L−1, respectively. Cell growth was still observed, and the final cellular concentration increased to 9.9 ± 0.5 g L−1 (Figure 3). Accordingly, the final concentration of succinic acid increased (from 6.1 ± 2.1 g L−1 in the first batch to 14.8 ± 0.9 g L−1 in the third sequential batch), that of acetic acid decreased (from 3.3 ± 0.4 g L−1 in the first batch to 2.0 ± 0.3 g L−1 in the third sequential batch) as well as that of propionic acid (from 39.5 ± 5.2 g L−1 in the first batch to 34.4 ± 1.9 g L−1 in the third sequential batch) in the sequential batches. A profile change was noticed in sorbitol consumption and product formation throughout the sequential batches, as shown in Figure 4. Furthermore, the overall final results and the carbon recovery for each sequential batch are summarized in Table 2.Figure 1


Microbial production of Propionic and Succinic acid from Sorbitol using Propionibacterium acidipropionici.

Duarte JC, Valença GP, Moran PJ, Rodrigues JA - AMB Express (2015)

Profile of sorbitol fermentation byP. acidipropionicifor the first batch operating in four independent vessels simultaneously.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4385012&req=5

Fig1: Profile of sorbitol fermentation byP. acidipropionicifor the first batch operating in four independent vessels simultaneously.
Mentions: Three sequential batches in two independent bioreactors, each one with two parallel vessels (four reactors) operating simultaneously, were carried out with P. acidipropionici ATCC 4875 using sorbitol as a carbon source. The results for the first batch are presented in Figure 1. It was observed that sorbitol was not completely consumed after 70 h, and its final concentration was 13.3 ± 0.9 g L−1. The final concentrations of succinic, acetic, and propionic acid were 6.1 ± 2.1, 3.3 ± 0.4, and 39.5 ± 5.2 g L−1, respectively. Cell growth was also observed, and the final cellular concentration was 4.3 ± 0.1 g L−1. In the second sequential batch, contrarily to the first batch, the substrate consumption was almost complete after 70 h, and its final concentration was 1.2 ± 1.3 g L−1. The final concentrations of succinic, acetic, and propionic acid were 10.0 ± 1.3, 2.2 ± 0.1, and 35.8 ± 1.4 g L−1, respectively. The final concentration of succinic acid increased while that of acetic and propionic acid decreased, compared with the first batch. As expected, cell growth was observed, and the final cellular concentration increased up to 6.1 ± 0.3 g L−1 (Figure 2). In the last sequential batch, substrate consumption was completed before 70 h, and the final concentrations of succinic, acetic, and propionic acid were 14.8 ± 0.9, 2.0 ± 0.3, and 34.4 ± 1.9 g L−1, respectively. Cell growth was still observed, and the final cellular concentration increased to 9.9 ± 0.5 g L−1 (Figure 3). Accordingly, the final concentration of succinic acid increased (from 6.1 ± 2.1 g L−1 in the first batch to 14.8 ± 0.9 g L−1 in the third sequential batch), that of acetic acid decreased (from 3.3 ± 0.4 g L−1 in the first batch to 2.0 ± 0.3 g L−1 in the third sequential batch) as well as that of propionic acid (from 39.5 ± 5.2 g L−1 in the first batch to 34.4 ± 1.9 g L−1 in the third sequential batch) in the sequential batches. A profile change was noticed in sorbitol consumption and product formation throughout the sequential batches, as shown in Figure 4. Furthermore, the overall final results and the carbon recovery for each sequential batch are summarized in Table 2.Figure 1

Bottom Line: The concentration of propionic acid decreased slightly from 39.5 ± 5.2 g L(-1) to 34.4 ± 1.9 g L(-1), and that of succinic acid increased significantly from 6.1 ± 2.1 g L(-1) to 14.8 ± 0.9 g L(-1) through the sequential batches.In addition, a small amount of acetic acid was produced that decreased from 3.3 ± 0.4 g L(-1) to 2.0 ± 0.3 g L(-1) through the batches.The major yield for propionic acid was 0.613 g g(-1) in the first batch and succinic acid it was 0.212 g g(-1) in the third batch.

View Article: PubMed Central - PubMed

Affiliation: Institute of Chemistry, University of Campinas, Campinas, SP 13083-970 Brazil ; School of Chemical Engineering, University of Campinas, Campinas, SP 13083-852 Brazil.

ABSTRACT
Three sequential fermentative batches were carried out with cell recycle in four simultaneously operating bioreactors maintained at pH 6.5, 30°C, and 100 rpm. P. acidipropionici ATCC 4875 was able to produce propionic and succinic acid from sorbitol. The concentration of propionic acid decreased slightly from 39.5 ± 5.2 g L(-1) to 34.4 ± 1.9 g L(-1), and that of succinic acid increased significantly from 6.1 ± 2.1 g L(-1) to 14.8 ± 0.9 g L(-1) through the sequential batches. In addition, a small amount of acetic acid was produced that decreased from 3.3 ± 0.4 g L(-1) to 2.0 ± 0.3 g L(-1) through the batches. The major yield for propionic acid was 0.613 g g(-1) in the first batch and succinic acid it was 0.212 g g(-1) in the third batch. The minor yield of acetic acid was 0.029 g g(-1), in the second and third batches.

No MeSH data available.


Related in: MedlinePlus