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Surface properties and biocompatibility of nanostructured TiO2 film deposited by RF magnetron sputtering.

Majeed A, He J, Jiao L, Zhong X, Sheng Z - Nanoscale Res Lett (2015)

Bottom Line: X-ray diffraction (XRD) analysis reveals that TiO2 films deposited on unbiased as well as biased substrates are all amorphous.Surface properties such as surface roughness and wettability of TiO2 films, grown in a plasma environment, under biased and unbiased substrate conditions are reported according to the said parameters of RF power and the working pressures.The effects of roughness and hydrophilicity of nanostructured TiO2 films on cell density and cell spreading have been discussed.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory for Laser Plasmas (Ministry of Education) and State Key Laboratory of Advanced Optical Communication Systems and Networks, Department of Physics and Astronomy, Shanghai Jiao Tong University, Shanghai, 200240 China ; Department of Physics, University of Azad Jammu & Kashmir, Muzaffarabad, A.K Pakistan.

ABSTRACT
Nanostructured TiO2 films are deposited on a silicon substrate using 150-W power from the RF magnetron sputtering at working pressures of 3 to 5 Pa, with no substrate bias, and at 3 Pa with a substrate bias of -50 V. X-ray diffraction (XRD) analysis reveals that TiO2 films deposited on unbiased as well as biased substrates are all amorphous. Surface properties such as surface roughness and wettability of TiO2 films, grown in a plasma environment, under biased and unbiased substrate conditions are reported according to the said parameters of RF power and the working pressures. Primary rat osteoblasts (MC3T3-E1) cells have been cultured on nanostructured TiO2 films fabricated at different conditions of substrate bias and working pressures. The effects of roughness and hydrophilicity of nanostructured TiO2 films on cell density and cell spreading have been discussed.

No MeSH data available.


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Images of primary rat osteoblasts (MC3T3-E1) on TiO2films. Images of primary rat osteoblasts (MC3T3-E1) on TiO2 films deposited at (a, b) 3 Pa, (c, d) 5 Pa, without biasing the substrate, and (e, f) 3 Pa at a substrate bias of −50 V.
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Fig6: Images of primary rat osteoblasts (MC3T3-E1) on TiO2films. Images of primary rat osteoblasts (MC3T3-E1) on TiO2 films deposited at (a, b) 3 Pa, (c, d) 5 Pa, without biasing the substrate, and (e, f) 3 Pa at a substrate bias of −50 V.

Mentions: The microscopic view of studying the morphology of PRO (MC3T3-E1) cells cultured on TiO2 samples prepared at(3, 5) Pa (no substrate bias) and 3 Pa (biased at −50 V) are shown in Figure 6. The cells on these samples are cultured for 12 h and are named as sample 1, 2, and 3, respectively. Having cultured the cells, there have been three kinds of cells attached onto the surface of each specimen/sample as shown in Figure 6. i) Not spread: cells are still spherical, protrusions are not yet produced; ii) partially spread: cells began to spread laterally with at one or more sides; and iii) fully spread: at this stage, the extensions of plasma membrane are completely confluent [6].Figure 6


Surface properties and biocompatibility of nanostructured TiO2 film deposited by RF magnetron sputtering.

Majeed A, He J, Jiao L, Zhong X, Sheng Z - Nanoscale Res Lett (2015)

Images of primary rat osteoblasts (MC3T3-E1) on TiO2films. Images of primary rat osteoblasts (MC3T3-E1) on TiO2 films deposited at (a, b) 3 Pa, (c, d) 5 Pa, without biasing the substrate, and (e, f) 3 Pa at a substrate bias of −50 V.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4385009&req=5

Fig6: Images of primary rat osteoblasts (MC3T3-E1) on TiO2films. Images of primary rat osteoblasts (MC3T3-E1) on TiO2 films deposited at (a, b) 3 Pa, (c, d) 5 Pa, without biasing the substrate, and (e, f) 3 Pa at a substrate bias of −50 V.
Mentions: The microscopic view of studying the morphology of PRO (MC3T3-E1) cells cultured on TiO2 samples prepared at(3, 5) Pa (no substrate bias) and 3 Pa (biased at −50 V) are shown in Figure 6. The cells on these samples are cultured for 12 h and are named as sample 1, 2, and 3, respectively. Having cultured the cells, there have been three kinds of cells attached onto the surface of each specimen/sample as shown in Figure 6. i) Not spread: cells are still spherical, protrusions are not yet produced; ii) partially spread: cells began to spread laterally with at one or more sides; and iii) fully spread: at this stage, the extensions of plasma membrane are completely confluent [6].Figure 6

Bottom Line: X-ray diffraction (XRD) analysis reveals that TiO2 films deposited on unbiased as well as biased substrates are all amorphous.Surface properties such as surface roughness and wettability of TiO2 films, grown in a plasma environment, under biased and unbiased substrate conditions are reported according to the said parameters of RF power and the working pressures.The effects of roughness and hydrophilicity of nanostructured TiO2 films on cell density and cell spreading have been discussed.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory for Laser Plasmas (Ministry of Education) and State Key Laboratory of Advanced Optical Communication Systems and Networks, Department of Physics and Astronomy, Shanghai Jiao Tong University, Shanghai, 200240 China ; Department of Physics, University of Azad Jammu & Kashmir, Muzaffarabad, A.K Pakistan.

ABSTRACT
Nanostructured TiO2 films are deposited on a silicon substrate using 150-W power from the RF magnetron sputtering at working pressures of 3 to 5 Pa, with no substrate bias, and at 3 Pa with a substrate bias of -50 V. X-ray diffraction (XRD) analysis reveals that TiO2 films deposited on unbiased as well as biased substrates are all amorphous. Surface properties such as surface roughness and wettability of TiO2 films, grown in a plasma environment, under biased and unbiased substrate conditions are reported according to the said parameters of RF power and the working pressures. Primary rat osteoblasts (MC3T3-E1) cells have been cultured on nanostructured TiO2 films fabricated at different conditions of substrate bias and working pressures. The effects of roughness and hydrophilicity of nanostructured TiO2 films on cell density and cell spreading have been discussed.

No MeSH data available.


Related in: MedlinePlus