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Multidimensional effects of biologically synthesized silver nanoparticles in Helicobacter pylori, Helicobacter felis, and human lung (L132) and lung carcinoma A549 cells.

Gurunathan S, Jeong JK, Han JW, Zhang XF, Park JH, Kim JH - Nanoscale Res Lett (2015)

Bottom Line: Furthermore, AgNPs induced mitochondrial-mediated apoptosis in A549 cells; conversely, AgNPs had no significant effects on L132 cells.Our findings demonstrate that this environmentally friendly method for the synthesis of AgNPs and that the prepared AgNPs have multidimensional effects such as anti-bacterial and anti-biofilm activity against H. pylori and H. felis and also cytotoxic effects against human cancer cells.This report describes comprehensively the effects of AgNPs on bacteria and mammalian cells.

View Article: PubMed Central - PubMed

Affiliation: Department of Animal Biotechnology, Konkuk University, 1 Hwayang-Dong, Gwanjgin-gu, 143-701 Seoul South Korea ; GS Institute of Bio and Nanotechnology, Coimbatore, Tamilnadu India.

ABSTRACT
Silver nanoparticles (AgNPs) are prominent group of nanomaterials and are recognized for their diverse applications in various health sectors. This study aimed to synthesize the AgNPs using the leaf extract of Artemisia princeps as a bio-reductant. Furthermore, we evaluated the multidimensional effect of the biologically synthesized AgNPs in Helicobacter pylori, Helicobacter felis, and human lung (L132) and lung carcinoma (A549) cells. UV-visible (UV-vis) spectroscopy confirmed the synthesis of AgNPs. X-ray diffraction (XRD) indicated that the AgNPs are specifically indexed to a crystal structure. The results from Fourier transform infrared spectroscopy (FTIR) indicate that biomolecules are involved in the synthesis and stabilization of AgNPs. Dynamic light scattering (DLS) studies showed the average size distribution of the particle between 10 and 40 nm, and transmission electron microscopy (TEM) confirmed that the AgNPs were significantly well separated and spherical with an average size of 20 nm. AgNPs caused dose-dependent decrease in cell viability and biofilm formation and increase in reactive oxygen species (ROS) generation and DNA fragmentation in H. pylori and H. felis. Furthermore, AgNPs induced mitochondrial-mediated apoptosis in A549 cells; conversely, AgNPs had no significant effects on L132 cells. The results from this study suggest that AgNPs could cause cell-specific apoptosis in mammalian cells. Our findings demonstrate that this environmentally friendly method for the synthesis of AgNPs and that the prepared AgNPs have multidimensional effects such as anti-bacterial and anti-biofilm activity against H. pylori and H. felis and also cytotoxic effects against human cancer cells. This report describes comprehensively the effects of AgNPs on bacteria and mammalian cells. We believe that biologically synthesized AgNPs will open a new avenue towards various biotechnological and biomedical applications in the near future.

No MeSH data available.


Related in: MedlinePlus

Time-dependent effect of AgNPs onH. pyloriandH. felis. Time-dependent effects of AgNPs on bacterial survival. All test strains were incubated in the presence of different concentrations of AgNPs. Bacterial survival was determined at 4 h by a CFU assay.
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Fig7: Time-dependent effect of AgNPs onH. pyloriandH. felis. Time-dependent effects of AgNPs on bacterial survival. All test strains were incubated in the presence of different concentrations of AgNPs. Bacterial survival was determined at 4 h by a CFU assay.

Mentions: The time-dependent antibacterial efficiency of AgNPs was determined in H. pylori and H. felis. The loss of viability of H. pylori and H. felis was counted at different time points such as 1, 2, 3, and 4 h (FigureĀ 7). The loss of H. pylori viability increased after 1-h incubation with AgNPs from 1.2 to 1.0 optical density, whereas H. felis shows a weak difference between treated and untreated; however, both strains showed a sharp decrease of growth from 1.0 to 0.6. By increasing the time of incubation, the loss of viability increased after 3 to 4 h of incubation. The division of cell death occurred in all 4 h of incubation; however, a large fraction of cell death occurred in the earlier hour of incubation. The treated groups show significant growth defect than control, whereas both control strains show health and no significant growth impairment.Figure 7


Multidimensional effects of biologically synthesized silver nanoparticles in Helicobacter pylori, Helicobacter felis, and human lung (L132) and lung carcinoma A549 cells.

Gurunathan S, Jeong JK, Han JW, Zhang XF, Park JH, Kim JH - Nanoscale Res Lett (2015)

Time-dependent effect of AgNPs onH. pyloriandH. felis. Time-dependent effects of AgNPs on bacterial survival. All test strains were incubated in the presence of different concentrations of AgNPs. Bacterial survival was determined at 4 h by a CFU assay.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4384991&req=5

Fig7: Time-dependent effect of AgNPs onH. pyloriandH. felis. Time-dependent effects of AgNPs on bacterial survival. All test strains were incubated in the presence of different concentrations of AgNPs. Bacterial survival was determined at 4 h by a CFU assay.
Mentions: The time-dependent antibacterial efficiency of AgNPs was determined in H. pylori and H. felis. The loss of viability of H. pylori and H. felis was counted at different time points such as 1, 2, 3, and 4 h (FigureĀ 7). The loss of H. pylori viability increased after 1-h incubation with AgNPs from 1.2 to 1.0 optical density, whereas H. felis shows a weak difference between treated and untreated; however, both strains showed a sharp decrease of growth from 1.0 to 0.6. By increasing the time of incubation, the loss of viability increased after 3 to 4 h of incubation. The division of cell death occurred in all 4 h of incubation; however, a large fraction of cell death occurred in the earlier hour of incubation. The treated groups show significant growth defect than control, whereas both control strains show health and no significant growth impairment.Figure 7

Bottom Line: Furthermore, AgNPs induced mitochondrial-mediated apoptosis in A549 cells; conversely, AgNPs had no significant effects on L132 cells.Our findings demonstrate that this environmentally friendly method for the synthesis of AgNPs and that the prepared AgNPs have multidimensional effects such as anti-bacterial and anti-biofilm activity against H. pylori and H. felis and also cytotoxic effects against human cancer cells.This report describes comprehensively the effects of AgNPs on bacteria and mammalian cells.

View Article: PubMed Central - PubMed

Affiliation: Department of Animal Biotechnology, Konkuk University, 1 Hwayang-Dong, Gwanjgin-gu, 143-701 Seoul South Korea ; GS Institute of Bio and Nanotechnology, Coimbatore, Tamilnadu India.

ABSTRACT
Silver nanoparticles (AgNPs) are prominent group of nanomaterials and are recognized for their diverse applications in various health sectors. This study aimed to synthesize the AgNPs using the leaf extract of Artemisia princeps as a bio-reductant. Furthermore, we evaluated the multidimensional effect of the biologically synthesized AgNPs in Helicobacter pylori, Helicobacter felis, and human lung (L132) and lung carcinoma (A549) cells. UV-visible (UV-vis) spectroscopy confirmed the synthesis of AgNPs. X-ray diffraction (XRD) indicated that the AgNPs are specifically indexed to a crystal structure. The results from Fourier transform infrared spectroscopy (FTIR) indicate that biomolecules are involved in the synthesis and stabilization of AgNPs. Dynamic light scattering (DLS) studies showed the average size distribution of the particle between 10 and 40 nm, and transmission electron microscopy (TEM) confirmed that the AgNPs were significantly well separated and spherical with an average size of 20 nm. AgNPs caused dose-dependent decrease in cell viability and biofilm formation and increase in reactive oxygen species (ROS) generation and DNA fragmentation in H. pylori and H. felis. Furthermore, AgNPs induced mitochondrial-mediated apoptosis in A549 cells; conversely, AgNPs had no significant effects on L132 cells. The results from this study suggest that AgNPs could cause cell-specific apoptosis in mammalian cells. Our findings demonstrate that this environmentally friendly method for the synthesis of AgNPs and that the prepared AgNPs have multidimensional effects such as anti-bacterial and anti-biofilm activity against H. pylori and H. felis and also cytotoxic effects against human cancer cells. This report describes comprehensively the effects of AgNPs on bacteria and mammalian cells. We believe that biologically synthesized AgNPs will open a new avenue towards various biotechnological and biomedical applications in the near future.

No MeSH data available.


Related in: MedlinePlus