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Multidimensional effects of biologically synthesized silver nanoparticles in Helicobacter pylori, Helicobacter felis, and human lung (L132) and lung carcinoma A549 cells.

Gurunathan S, Jeong JK, Han JW, Zhang XF, Park JH, Kim JH - Nanoscale Res Lett (2015)

Bottom Line: Furthermore, AgNPs induced mitochondrial-mediated apoptosis in A549 cells; conversely, AgNPs had no significant effects on L132 cells.Our findings demonstrate that this environmentally friendly method for the synthesis of AgNPs and that the prepared AgNPs have multidimensional effects such as anti-bacterial and anti-biofilm activity against H. pylori and H. felis and also cytotoxic effects against human cancer cells.This report describes comprehensively the effects of AgNPs on bacteria and mammalian cells.

View Article: PubMed Central - PubMed

Affiliation: Department of Animal Biotechnology, Konkuk University, 1 Hwayang-Dong, Gwanjgin-gu, 143-701 Seoul South Korea ; GS Institute of Bio and Nanotechnology, Coimbatore, Tamilnadu India.

ABSTRACT
Silver nanoparticles (AgNPs) are prominent group of nanomaterials and are recognized for their diverse applications in various health sectors. This study aimed to synthesize the AgNPs using the leaf extract of Artemisia princeps as a bio-reductant. Furthermore, we evaluated the multidimensional effect of the biologically synthesized AgNPs in Helicobacter pylori, Helicobacter felis, and human lung (L132) and lung carcinoma (A549) cells. UV-visible (UV-vis) spectroscopy confirmed the synthesis of AgNPs. X-ray diffraction (XRD) indicated that the AgNPs are specifically indexed to a crystal structure. The results from Fourier transform infrared spectroscopy (FTIR) indicate that biomolecules are involved in the synthesis and stabilization of AgNPs. Dynamic light scattering (DLS) studies showed the average size distribution of the particle between 10 and 40 nm, and transmission electron microscopy (TEM) confirmed that the AgNPs were significantly well separated and spherical with an average size of 20 nm. AgNPs caused dose-dependent decrease in cell viability and biofilm formation and increase in reactive oxygen species (ROS) generation and DNA fragmentation in H. pylori and H. felis. Furthermore, AgNPs induced mitochondrial-mediated apoptosis in A549 cells; conversely, AgNPs had no significant effects on L132 cells. The results from this study suggest that AgNPs could cause cell-specific apoptosis in mammalian cells. Our findings demonstrate that this environmentally friendly method for the synthesis of AgNPs and that the prepared AgNPs have multidimensional effects such as anti-bacterial and anti-biofilm activity against H. pylori and H. felis and also cytotoxic effects against human cancer cells. This report describes comprehensively the effects of AgNPs on bacteria and mammalian cells. We believe that biologically synthesized AgNPs will open a new avenue towards various biotechnological and biomedical applications in the near future.

No MeSH data available.


Related in: MedlinePlus

XRD pattern of AgNPs. A representative X-ray diffraction (XRD) pattern of silver nanoparticles formed after reaction of leaf extract with 1 mM of silver nitrate (AgNO3) for 60 min at 60°C. The XRD pattern shows two intense peaks in the whole spectrum of 2θ values ranging from 20 to 70. The intense peaks were observed at 2θ values of 31.9 and 45.31 corresponding to (111) and (200) planes for silver, respectively.
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Fig2: XRD pattern of AgNPs. A representative X-ray diffraction (XRD) pattern of silver nanoparticles formed after reaction of leaf extract with 1 mM of silver nitrate (AgNO3) for 60 min at 60°C. The XRD pattern shows two intense peaks in the whole spectrum of 2θ values ranging from 20 to 70. The intense peaks were observed at 2θ values of 31.9 and 45.31 corresponding to (111) and (200) planes for silver, respectively.

Mentions: The crystalline nature of the AgNPs was confirmed using XRD, and the XRD pattern revealed Bragg’s reflections that represent the face-centered cubic structure of silver. Figure 2 shows the XRD patterns of dried AgNPs synthesized with A. princeps leaf extract. The XRD patterns indicated that the structure of AgNPs is face-centered cubic (fcc) [8,57]. The sharp XRD peaks at 2θ of 31.9 and 45.3 are attributed to the (111) and (200) crystallographic planes. The two diffraction peaks could be indexed as (111), (200), (220), (311), and (222) planes of fcc silver (JCPDS, fileno.04-0783) [24]. A comparison made between our XRD spectrum and the standard confirmed that our silver particles exhibited Bragg’s reflections of silver. Hence, the XRD results clearly show that the AgNPs are crystalline. Interestingly, the XRD pattern shows no impurities as reported earlier [58]. From the XRD data and the use of the Debye-Scherer equation, the average particle size was 20 ± 3.5 nm. Remarkably, the absence of peaks from other phases suggests that nanoparticles with single-phase Ag with cubic structure were synthesized. Awwad et al. [58] reported that using carob leaf extract as a reducing and stabilizing agent produced 5- to 50-nm sizes of AgNPs. Recently, Mukherjee et al. [59] reported that the AgNPs synthesized with leaf extract from Olax scandens were mostly monodispersed and spherical (20 to 60 nm) along with very few larger particles (approximately 90 nm). Our studies suggest that A. princeps shows a significantly uniform distribution of particles with an average size of 20 nm.Figure 2


Multidimensional effects of biologically synthesized silver nanoparticles in Helicobacter pylori, Helicobacter felis, and human lung (L132) and lung carcinoma A549 cells.

Gurunathan S, Jeong JK, Han JW, Zhang XF, Park JH, Kim JH - Nanoscale Res Lett (2015)

XRD pattern of AgNPs. A representative X-ray diffraction (XRD) pattern of silver nanoparticles formed after reaction of leaf extract with 1 mM of silver nitrate (AgNO3) for 60 min at 60°C. The XRD pattern shows two intense peaks in the whole spectrum of 2θ values ranging from 20 to 70. The intense peaks were observed at 2θ values of 31.9 and 45.31 corresponding to (111) and (200) planes for silver, respectively.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4384991&req=5

Fig2: XRD pattern of AgNPs. A representative X-ray diffraction (XRD) pattern of silver nanoparticles formed after reaction of leaf extract with 1 mM of silver nitrate (AgNO3) for 60 min at 60°C. The XRD pattern shows two intense peaks in the whole spectrum of 2θ values ranging from 20 to 70. The intense peaks were observed at 2θ values of 31.9 and 45.31 corresponding to (111) and (200) planes for silver, respectively.
Mentions: The crystalline nature of the AgNPs was confirmed using XRD, and the XRD pattern revealed Bragg’s reflections that represent the face-centered cubic structure of silver. Figure 2 shows the XRD patterns of dried AgNPs synthesized with A. princeps leaf extract. The XRD patterns indicated that the structure of AgNPs is face-centered cubic (fcc) [8,57]. The sharp XRD peaks at 2θ of 31.9 and 45.3 are attributed to the (111) and (200) crystallographic planes. The two diffraction peaks could be indexed as (111), (200), (220), (311), and (222) planes of fcc silver (JCPDS, fileno.04-0783) [24]. A comparison made between our XRD spectrum and the standard confirmed that our silver particles exhibited Bragg’s reflections of silver. Hence, the XRD results clearly show that the AgNPs are crystalline. Interestingly, the XRD pattern shows no impurities as reported earlier [58]. From the XRD data and the use of the Debye-Scherer equation, the average particle size was 20 ± 3.5 nm. Remarkably, the absence of peaks from other phases suggests that nanoparticles with single-phase Ag with cubic structure were synthesized. Awwad et al. [58] reported that using carob leaf extract as a reducing and stabilizing agent produced 5- to 50-nm sizes of AgNPs. Recently, Mukherjee et al. [59] reported that the AgNPs synthesized with leaf extract from Olax scandens were mostly monodispersed and spherical (20 to 60 nm) along with very few larger particles (approximately 90 nm). Our studies suggest that A. princeps shows a significantly uniform distribution of particles with an average size of 20 nm.Figure 2

Bottom Line: Furthermore, AgNPs induced mitochondrial-mediated apoptosis in A549 cells; conversely, AgNPs had no significant effects on L132 cells.Our findings demonstrate that this environmentally friendly method for the synthesis of AgNPs and that the prepared AgNPs have multidimensional effects such as anti-bacterial and anti-biofilm activity against H. pylori and H. felis and also cytotoxic effects against human cancer cells.This report describes comprehensively the effects of AgNPs on bacteria and mammalian cells.

View Article: PubMed Central - PubMed

Affiliation: Department of Animal Biotechnology, Konkuk University, 1 Hwayang-Dong, Gwanjgin-gu, 143-701 Seoul South Korea ; GS Institute of Bio and Nanotechnology, Coimbatore, Tamilnadu India.

ABSTRACT
Silver nanoparticles (AgNPs) are prominent group of nanomaterials and are recognized for their diverse applications in various health sectors. This study aimed to synthesize the AgNPs using the leaf extract of Artemisia princeps as a bio-reductant. Furthermore, we evaluated the multidimensional effect of the biologically synthesized AgNPs in Helicobacter pylori, Helicobacter felis, and human lung (L132) and lung carcinoma (A549) cells. UV-visible (UV-vis) spectroscopy confirmed the synthesis of AgNPs. X-ray diffraction (XRD) indicated that the AgNPs are specifically indexed to a crystal structure. The results from Fourier transform infrared spectroscopy (FTIR) indicate that biomolecules are involved in the synthesis and stabilization of AgNPs. Dynamic light scattering (DLS) studies showed the average size distribution of the particle between 10 and 40 nm, and transmission electron microscopy (TEM) confirmed that the AgNPs were significantly well separated and spherical with an average size of 20 nm. AgNPs caused dose-dependent decrease in cell viability and biofilm formation and increase in reactive oxygen species (ROS) generation and DNA fragmentation in H. pylori and H. felis. Furthermore, AgNPs induced mitochondrial-mediated apoptosis in A549 cells; conversely, AgNPs had no significant effects on L132 cells. The results from this study suggest that AgNPs could cause cell-specific apoptosis in mammalian cells. Our findings demonstrate that this environmentally friendly method for the synthesis of AgNPs and that the prepared AgNPs have multidimensional effects such as anti-bacterial and anti-biofilm activity against H. pylori and H. felis and also cytotoxic effects against human cancer cells. This report describes comprehensively the effects of AgNPs on bacteria and mammalian cells. We believe that biologically synthesized AgNPs will open a new avenue towards various biotechnological and biomedical applications in the near future.

No MeSH data available.


Related in: MedlinePlus