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Human-like collagen protein-coated magnetic nanoparticles with high magnetic hyperthermia performance and improved biocompatibility.

Liu X, Zhang H, Chang L, Yu B, Liu Q, Wu J, Miao Y, Ma P, Fan D, Fan H - Nanoscale Res Lett (2015)

Bottom Line: Human-like collagen (HLC)-coated monodispersed superparamagnetic Fe3O4 nanoparticles have been successfully prepared to investigate its effect on heat induction property and cell toxicity.After coating of HLC, the sample shows a faster rate of temperature increase under an alternating magnetic field although it has a reduced saturation magnetization.Our results clearly show that Fe3O4 nanoparticles after coating with HLC not only possess effective heat induction for cancer treatment but also have improved biocompatibility for biomedicine applications.

View Article: PubMed Central - PubMed

Affiliation: Shaanxi Key Laboratory of Degradable Biomedical Materials, School of Chemical Engineering, Northwest University, Taibai North Road 229, Xi'an, Shaanxi 710069 China ; Department of Materials Science and Engineering, Faculty of Engineering, National University of Singapore, 7 Engineering Drive 1, Singapore, 117574 Singapore.

ABSTRACT
Human-like collagen (HLC)-coated monodispersed superparamagnetic Fe3O4 nanoparticles have been successfully prepared to investigate its effect on heat induction property and cell toxicity. After coating of HLC, the sample shows a faster rate of temperature increase under an alternating magnetic field although it has a reduced saturation magnetization. This is most probably a result of the effective heat conduction and good colloid stability due to the high charge of HLC on the surface. In addition, compared with Fe3O4 nanoparticles before coating with HLC, HLC-coated Fe3O4 nanoparticles do not induce notable cytotoxic effect at higher concentration which indicates that HLC-coated Fe3O4 nanoparticles has improved biocompatibility. Our results clearly show that Fe3O4 nanoparticles after coating with HLC not only possess effective heat induction for cancer treatment but also have improved biocompatibility for biomedicine applications.

No MeSH data available.


Related in: MedlinePlus

Cell viability. Cell viability data of Fe3O4 NPs before and after coating HLC obtained from cultured NIH3T3 cells using standard CCK-8 colorimetric assays. Error bars, SEM; *p < 0.05; **p < 0.01; ***p < 0.001.
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Fig7: Cell viability. Cell viability data of Fe3O4 NPs before and after coating HLC obtained from cultured NIH3T3 cells using standard CCK-8 colorimetric assays. Error bars, SEM; *p < 0.05; **p < 0.01; ***p < 0.001.

Mentions: Before being used for practical applications, it is important to evaluate the biocompatibility of HLC-coated Fe3O4 NPs. The cell viabilities were determined after a 24-h co-incubation with fibroblast NIH3T3 cells. As can be seen in Figure 7, the observed cytotoxicity increased with increasing Fe concentration. At 25 to 100 μg/mL of Fe, Fe3O4 NPs both before and after coating with HLC showed no obvious decrease in the viability of the NIH3T3 cells. Fe3O4 NPs before coating with HLC induced a cytotoxic effect in NIH3T3 cells at a concentration of 250 μg/mL Fe. However, HLC-coated Fe3O4 NPs did not induce notable cytotoxic effect between 100 to 250 μg/mL Fe. Thus, HLC has excellent biocompatibility. This means that Fe3O4 NPs after coating with HLC clearly exhibit better biocompatibility than uncoated Fe3O4 NPs, especially at high concentrations. These results suggest that HLC-coated Fe3O4 NPs are excellent candidates for further practical applications.Figure 7


Human-like collagen protein-coated magnetic nanoparticles with high magnetic hyperthermia performance and improved biocompatibility.

Liu X, Zhang H, Chang L, Yu B, Liu Q, Wu J, Miao Y, Ma P, Fan D, Fan H - Nanoscale Res Lett (2015)

Cell viability. Cell viability data of Fe3O4 NPs before and after coating HLC obtained from cultured NIH3T3 cells using standard CCK-8 colorimetric assays. Error bars, SEM; *p < 0.05; **p < 0.01; ***p < 0.001.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4384969&req=5

Fig7: Cell viability. Cell viability data of Fe3O4 NPs before and after coating HLC obtained from cultured NIH3T3 cells using standard CCK-8 colorimetric assays. Error bars, SEM; *p < 0.05; **p < 0.01; ***p < 0.001.
Mentions: Before being used for practical applications, it is important to evaluate the biocompatibility of HLC-coated Fe3O4 NPs. The cell viabilities were determined after a 24-h co-incubation with fibroblast NIH3T3 cells. As can be seen in Figure 7, the observed cytotoxicity increased with increasing Fe concentration. At 25 to 100 μg/mL of Fe, Fe3O4 NPs both before and after coating with HLC showed no obvious decrease in the viability of the NIH3T3 cells. Fe3O4 NPs before coating with HLC induced a cytotoxic effect in NIH3T3 cells at a concentration of 250 μg/mL Fe. However, HLC-coated Fe3O4 NPs did not induce notable cytotoxic effect between 100 to 250 μg/mL Fe. Thus, HLC has excellent biocompatibility. This means that Fe3O4 NPs after coating with HLC clearly exhibit better biocompatibility than uncoated Fe3O4 NPs, especially at high concentrations. These results suggest that HLC-coated Fe3O4 NPs are excellent candidates for further practical applications.Figure 7

Bottom Line: Human-like collagen (HLC)-coated monodispersed superparamagnetic Fe3O4 nanoparticles have been successfully prepared to investigate its effect on heat induction property and cell toxicity.After coating of HLC, the sample shows a faster rate of temperature increase under an alternating magnetic field although it has a reduced saturation magnetization.Our results clearly show that Fe3O4 nanoparticles after coating with HLC not only possess effective heat induction for cancer treatment but also have improved biocompatibility for biomedicine applications.

View Article: PubMed Central - PubMed

Affiliation: Shaanxi Key Laboratory of Degradable Biomedical Materials, School of Chemical Engineering, Northwest University, Taibai North Road 229, Xi'an, Shaanxi 710069 China ; Department of Materials Science and Engineering, Faculty of Engineering, National University of Singapore, 7 Engineering Drive 1, Singapore, 117574 Singapore.

ABSTRACT
Human-like collagen (HLC)-coated monodispersed superparamagnetic Fe3O4 nanoparticles have been successfully prepared to investigate its effect on heat induction property and cell toxicity. After coating of HLC, the sample shows a faster rate of temperature increase under an alternating magnetic field although it has a reduced saturation magnetization. This is most probably a result of the effective heat conduction and good colloid stability due to the high charge of HLC on the surface. In addition, compared with Fe3O4 nanoparticles before coating with HLC, HLC-coated Fe3O4 nanoparticles do not induce notable cytotoxic effect at higher concentration which indicates that HLC-coated Fe3O4 nanoparticles has improved biocompatibility. Our results clearly show that Fe3O4 nanoparticles after coating with HLC not only possess effective heat induction for cancer treatment but also have improved biocompatibility for biomedicine applications.

No MeSH data available.


Related in: MedlinePlus