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Application of a new xylanase activity from Bacillus amyloliquefaciens XR44A in brewer's spent grain saccharification.

Amore A, Parameswaran B, Kumar R, Birolo L, Vinciguerra R, Marcolongo L, Ionata E, La Cara F, Pandey A, Faraco V - J Chem Technol Biotechnol (2014)

Bottom Line: A novel endo-1,4-beta xylanase was identified combining zymography and proteomics and recognized as the main enzyme responsible for B. amyloliquefaciens XR44A xylanase activity.The new xylanase activity was partially characterized and its application in saccharification of brewer's spent grain, pretreated by aqueous ammonia soaking, was investigated.Journal of Chemical Technology & Biotechnology published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.

View Article: PubMed Central - PubMed

Affiliation: Department of Chemical Sciences, University of Naples "Federico II", Complesso Universitario Monte S. Angelo via Cintia, 4, 80126, Naples, Italy.

ABSTRACT

Background: Cellulases and xylanases are the key enzymes involved in the conversion of lignocelluloses into fermentable sugars. Western Ghat region (India) has been recognized as an active hot spot for the isolation of new microorganisms. The aim of this work was to isolate new microorganisms producing cellulases and xylanases to be applied in brewer's spent grain saccharification.

Results: 93 microorganisms were isolated from Western Ghat and screened for the production of cellulase and xylanase activities. Fourteen cellulolytic and seven xylanolytic microorganisms were further screened in liquid culture. Particular attention was focused on the new isolate Bacillus amyloliquefaciens XR44A, producing xylanase activity up to 10.5 U mL(-1). A novel endo-1,4-beta xylanase was identified combining zymography and proteomics and recognized as the main enzyme responsible for B. amyloliquefaciens XR44A xylanase activity. The new xylanase activity was partially characterized and its application in saccharification of brewer's spent grain, pretreated by aqueous ammonia soaking, was investigated.

Conclusion: The culture supernatant of B. amyloliquefaciens XR44A with xylanase activity allowed a recovery of around 43% xylose during brewer's spent grain saccharification, similar to the value obtained with a commercial xylanase from Trichoderma viride, and a maximum arabinose yield of 92%, around 2-fold higher than that achieved with the commercial xylanase. © 2014 The Authors. Journal of Chemical Technology & Biotechnology published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.

No MeSH data available.


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Screening of cellulolytic (A) and xylanolytic (B) strains in liquid medium.
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fig01: Screening of cellulolytic (A) and xylanolytic (B) strains in liquid medium.

Mentions: A further screening of the seven xylanolytic and fourteen cellulolytic strains was carried out in liquid culture, using a 1% xylan- and 1% CMC-containing medium, respectively. Xylanase (Fig. 1(A)) and CMCase activity (Fig. 1(B)) were assayed after 6 and 24 h of incubation. Eight microorganisms (R5A, R9A, R10A, R10B, R13A, R13B, R14B, R21A) were identified as the best producers of the cellulase activity with values of AZO-CMCase activity ranging from 0.045 up to 0.075 U mL−1, after 6 h incubation. XR18B, XR44A, XR76A and XR108A produced the highest levels of xylanase activity, ranging from 0.4 to 0.6 UmL−1, similarly to other xylanase-producing Bacillus strains.10,13


Application of a new xylanase activity from Bacillus amyloliquefaciens XR44A in brewer's spent grain saccharification.

Amore A, Parameswaran B, Kumar R, Birolo L, Vinciguerra R, Marcolongo L, Ionata E, La Cara F, Pandey A, Faraco V - J Chem Technol Biotechnol (2014)

Screening of cellulolytic (A) and xylanolytic (B) strains in liquid medium.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4384805&req=5

fig01: Screening of cellulolytic (A) and xylanolytic (B) strains in liquid medium.
Mentions: A further screening of the seven xylanolytic and fourteen cellulolytic strains was carried out in liquid culture, using a 1% xylan- and 1% CMC-containing medium, respectively. Xylanase (Fig. 1(A)) and CMCase activity (Fig. 1(B)) were assayed after 6 and 24 h of incubation. Eight microorganisms (R5A, R9A, R10A, R10B, R13A, R13B, R14B, R21A) were identified as the best producers of the cellulase activity with values of AZO-CMCase activity ranging from 0.045 up to 0.075 U mL−1, after 6 h incubation. XR18B, XR44A, XR76A and XR108A produced the highest levels of xylanase activity, ranging from 0.4 to 0.6 UmL−1, similarly to other xylanase-producing Bacillus strains.10,13

Bottom Line: A novel endo-1,4-beta xylanase was identified combining zymography and proteomics and recognized as the main enzyme responsible for B. amyloliquefaciens XR44A xylanase activity.The new xylanase activity was partially characterized and its application in saccharification of brewer's spent grain, pretreated by aqueous ammonia soaking, was investigated.Journal of Chemical Technology & Biotechnology published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.

View Article: PubMed Central - PubMed

Affiliation: Department of Chemical Sciences, University of Naples "Federico II", Complesso Universitario Monte S. Angelo via Cintia, 4, 80126, Naples, Italy.

ABSTRACT

Background: Cellulases and xylanases are the key enzymes involved in the conversion of lignocelluloses into fermentable sugars. Western Ghat region (India) has been recognized as an active hot spot for the isolation of new microorganisms. The aim of this work was to isolate new microorganisms producing cellulases and xylanases to be applied in brewer's spent grain saccharification.

Results: 93 microorganisms were isolated from Western Ghat and screened for the production of cellulase and xylanase activities. Fourteen cellulolytic and seven xylanolytic microorganisms were further screened in liquid culture. Particular attention was focused on the new isolate Bacillus amyloliquefaciens XR44A, producing xylanase activity up to 10.5 U mL(-1). A novel endo-1,4-beta xylanase was identified combining zymography and proteomics and recognized as the main enzyme responsible for B. amyloliquefaciens XR44A xylanase activity. The new xylanase activity was partially characterized and its application in saccharification of brewer's spent grain, pretreated by aqueous ammonia soaking, was investigated.

Conclusion: The culture supernatant of B. amyloliquefaciens XR44A with xylanase activity allowed a recovery of around 43% xylose during brewer's spent grain saccharification, similar to the value obtained with a commercial xylanase from Trichoderma viride, and a maximum arabinose yield of 92%, around 2-fold higher than that achieved with the commercial xylanase. © 2014 The Authors. Journal of Chemical Technology & Biotechnology published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.

No MeSH data available.


Related in: MedlinePlus