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Occurrence and molecular identification of Giardia duodenalis from stray cats in Guangzhou, southern China.

Zheng G, Hu W, Liu Y, Luo Q, Tan L, Li G - Korean J. Parasitol. (2015)

Bottom Line: The phylogenetic trees were constructed using obtained sequences by MEGA5.2 software.Results show that 9.8% (10/102) feline fecal samples were found to be positive by microscopy, 10% (3/30) in Baiyun district and 9.7% (7/72) in Conghua district.Based on tpi, gdh, and bg genes, all sequences of assemblage A showed complete homology with AI except for 1 isolate (CHC83).

View Article: PubMed Central - PubMed

Affiliation: College of Veterinary Medicine, South China Agricultural University, Guangzhou, 510642, People's Republic of China.

ABSTRACT
The objective of this study was to genetically characterize isolates of Giardia duodenalis and to determine if zoonotic potential of G. duodenalis could be found in stray cats from urban and suburban environments in Guangzhou, China. Among 102 fresh fecal samples of stray cats, 30 samples were collected in Baiyun district (urban) and 72 in Conghua district (suburban). G. duodenalis specimens were examined using light microscopy, then the positive specimens were subjected to PCR amplification and subsequent sequencing at 4 loci such as glutamate dehydrogenase (gdh), triose phosphate isomerase (tpi), β-giardin (bg), and small subunit ribosomal RNA (18S rRNA) genes. The phylogenetic trees were constructed using obtained sequences by MEGA5.2 software. Results show that 9.8% (10/102) feline fecal samples were found to be positive by microscopy, 10% (3/30) in Baiyun district and 9.7% (7/72) in Conghua district. Among the 10 positive samples, 9 were single infection (8 isolates, assemblage A; 1 isolate, assemblage F) and 1 sample was mixed infection with assemblages A and C. Based on tpi, gdh, and bg genes, all sequences of assemblage A showed complete homology with AI except for 1 isolate (CHC83). These findings not only confirmed the occurrence of G. duodenalis in stray cats, but also showed that zoonotic assemblage A was found for the first time in stray cats living in urban and suburban environments in China.

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Phylogenetic relationships of Giardia duodenalis assemblages from cats inferred by the neighbor-joining analysis of 4 gene loci. (A) Triose phosphate isomerase (tpi) gene. (B) Glutamate dehydrogenase (gdh) gene. (C) β-giardin (bg) gene. (D) Small subunit ribosomal RNA (18S rRNA) gene. Bootstrap values obtained from 1,000 replicates are indicated on branches in percentage.
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f2-kjp-53-1-119: Phylogenetic relationships of Giardia duodenalis assemblages from cats inferred by the neighbor-joining analysis of 4 gene loci. (A) Triose phosphate isomerase (tpi) gene. (B) Glutamate dehydrogenase (gdh) gene. (C) β-giardin (bg) gene. (D) Small subunit ribosomal RNA (18S rRNA) gene. Bootstrap values obtained from 1,000 replicates are indicated on branches in percentage.

Mentions: The sequences of the 4 genes were included in the phylogenetic tree and homologous analysis, which were performed by using neighbor joining method and nucleotide base alignment, respectively. All sequences of assemblage A were placed in 1 cluster with AI reference sequence (GU564275) separating from AII and AIII at tpi gene by the neighbor joining tree with high bootstrap support (Fig. 2A). At gdh gene, the neighbor joining tree placed the 4 sequences (CHC62, CHC66, CHC77, and CHC83) in 1 cluster with AI reference sequence (EF685696) with high bootstrap support (Fig. 2B). Assemblage A, C, and F were significantly separated from each other at bg gene (Fig. 2C). All sequences of assemblage A (CBY20, CHC68, CHC77, and CHC79) were placed in 1 cluster with AI reference sequence (X85958) separating from AII and AIII. Assemblage A and F were also significantly separated from each other at 18S rRNA gene (Fig. 2D). Based on tpi, gdh, and bg genes, all sequences of assemblage A showed complete homology with AI (GU564275, EF685696, and X85958, respectively) except for 1 isolate (CHC83) where substitution at 1 position was observed (T-G at position 25) (Table 2).


Occurrence and molecular identification of Giardia duodenalis from stray cats in Guangzhou, southern China.

Zheng G, Hu W, Liu Y, Luo Q, Tan L, Li G - Korean J. Parasitol. (2015)

Phylogenetic relationships of Giardia duodenalis assemblages from cats inferred by the neighbor-joining analysis of 4 gene loci. (A) Triose phosphate isomerase (tpi) gene. (B) Glutamate dehydrogenase (gdh) gene. (C) β-giardin (bg) gene. (D) Small subunit ribosomal RNA (18S rRNA) gene. Bootstrap values obtained from 1,000 replicates are indicated on branches in percentage.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4384803&req=5

f2-kjp-53-1-119: Phylogenetic relationships of Giardia duodenalis assemblages from cats inferred by the neighbor-joining analysis of 4 gene loci. (A) Triose phosphate isomerase (tpi) gene. (B) Glutamate dehydrogenase (gdh) gene. (C) β-giardin (bg) gene. (D) Small subunit ribosomal RNA (18S rRNA) gene. Bootstrap values obtained from 1,000 replicates are indicated on branches in percentage.
Mentions: The sequences of the 4 genes were included in the phylogenetic tree and homologous analysis, which were performed by using neighbor joining method and nucleotide base alignment, respectively. All sequences of assemblage A were placed in 1 cluster with AI reference sequence (GU564275) separating from AII and AIII at tpi gene by the neighbor joining tree with high bootstrap support (Fig. 2A). At gdh gene, the neighbor joining tree placed the 4 sequences (CHC62, CHC66, CHC77, and CHC83) in 1 cluster with AI reference sequence (EF685696) with high bootstrap support (Fig. 2B). Assemblage A, C, and F were significantly separated from each other at bg gene (Fig. 2C). All sequences of assemblage A (CBY20, CHC68, CHC77, and CHC79) were placed in 1 cluster with AI reference sequence (X85958) separating from AII and AIII. Assemblage A and F were also significantly separated from each other at 18S rRNA gene (Fig. 2D). Based on tpi, gdh, and bg genes, all sequences of assemblage A showed complete homology with AI (GU564275, EF685696, and X85958, respectively) except for 1 isolate (CHC83) where substitution at 1 position was observed (T-G at position 25) (Table 2).

Bottom Line: The phylogenetic trees were constructed using obtained sequences by MEGA5.2 software.Results show that 9.8% (10/102) feline fecal samples were found to be positive by microscopy, 10% (3/30) in Baiyun district and 9.7% (7/72) in Conghua district.Based on tpi, gdh, and bg genes, all sequences of assemblage A showed complete homology with AI except for 1 isolate (CHC83).

View Article: PubMed Central - PubMed

Affiliation: College of Veterinary Medicine, South China Agricultural University, Guangzhou, 510642, People's Republic of China.

ABSTRACT
The objective of this study was to genetically characterize isolates of Giardia duodenalis and to determine if zoonotic potential of G. duodenalis could be found in stray cats from urban and suburban environments in Guangzhou, China. Among 102 fresh fecal samples of stray cats, 30 samples were collected in Baiyun district (urban) and 72 in Conghua district (suburban). G. duodenalis specimens were examined using light microscopy, then the positive specimens were subjected to PCR amplification and subsequent sequencing at 4 loci such as glutamate dehydrogenase (gdh), triose phosphate isomerase (tpi), β-giardin (bg), and small subunit ribosomal RNA (18S rRNA) genes. The phylogenetic trees were constructed using obtained sequences by MEGA5.2 software. Results show that 9.8% (10/102) feline fecal samples were found to be positive by microscopy, 10% (3/30) in Baiyun district and 9.7% (7/72) in Conghua district. Among the 10 positive samples, 9 were single infection (8 isolates, assemblage A; 1 isolate, assemblage F) and 1 sample was mixed infection with assemblages A and C. Based on tpi, gdh, and bg genes, all sequences of assemblage A showed complete homology with AI except for 1 isolate (CHC83). These findings not only confirmed the occurrence of G. duodenalis in stray cats, but also showed that zoonotic assemblage A was found for the first time in stray cats living in urban and suburban environments in China.

Show MeSH
Related in: MedlinePlus