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Occurrence and molecular identification of Giardia duodenalis from stray cats in Guangzhou, southern China.

Zheng G, Hu W, Liu Y, Luo Q, Tan L, Li G - Korean J. Parasitol. (2015)

Bottom Line: Results show that 9.8% (10/102) feline fecal samples were found to be positive by microscopy, 10% (3/30) in Baiyun district and 9.7% (7/72) in Conghua district.Among the 10 positive samples, 9 were single infection (8 isolates, assemblage A; 1 isolate, assemblage F) and 1 sample was mixed infection with assemblages A and C.Based on tpi, gdh, and bg genes, all sequences of assemblage A showed complete homology with AI except for 1 isolate (CHC83).

View Article: PubMed Central - PubMed

Affiliation: College of Veterinary Medicine, South China Agricultural University, Guangzhou, 510642, People's Republic of China.

ABSTRACT
The objective of this study was to genetically characterize isolates of Giardia duodenalis and to determine if zoonotic potential of G. duodenalis could be found in stray cats from urban and suburban environments in Guangzhou, China. Among 102 fresh fecal samples of stray cats, 30 samples were collected in Baiyun district (urban) and 72 in Conghua district (suburban). G. duodenalis specimens were examined using light microscopy, then the positive specimens were subjected to PCR amplification and subsequent sequencing at 4 loci such as glutamate dehydrogenase (gdh), triose phosphate isomerase (tpi), β-giardin (bg), and small subunit ribosomal RNA (18S rRNA) genes. The phylogenetic trees were constructed using obtained sequences by MEGA5.2 software. Results show that 9.8% (10/102) feline fecal samples were found to be positive by microscopy, 10% (3/30) in Baiyun district and 9.7% (7/72) in Conghua district. Among the 10 positive samples, 9 were single infection (8 isolates, assemblage A; 1 isolate, assemblage F) and 1 sample was mixed infection with assemblages A and C. Based on tpi, gdh, and bg genes, all sequences of assemblage A showed complete homology with AI except for 1 isolate (CHC83). These findings not only confirmed the occurrence of G. duodenalis in stray cats, but also showed that zoonotic assemblage A was found for the first time in stray cats living in urban and suburban environments in China.

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PCR amplification of tpi (A), gdh (B), bg (C), and 18S rRNA (D) genes from the cat-derived Giardia. M, DL2000 DNA marker; 1,15-CBY19; 2,16-CBY20; 3,17-CHC79; 4-CHC83; 5,11,21- CHC66; 6-CHC77; 7,13-CHC62; 8-CHC80; 9,19,22-CHC68; 10-CHC83; 12,18-CHC77; 14,20-CBY01.
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f1-kjp-53-1-119: PCR amplification of tpi (A), gdh (B), bg (C), and 18S rRNA (D) genes from the cat-derived Giardia. M, DL2000 DNA marker; 1,15-CBY19; 2,16-CBY20; 3,17-CHC79; 4-CHC83; 5,11,21- CHC66; 6-CHC77; 7,13-CHC62; 8-CHC80; 9,19,22-CHC68; 10-CHC83; 12,18-CHC77; 14,20-CBY01.

Mentions: The amplification fragments of the tpi (334 bp), gdh (530 bp), bg (515 bp), and 18S rRNA (291 bp) genes were obtained from 9, 4, 6, and 3 feline samples collected in Guangzhou, respectively (Fig. 1). The assemblages or sub-assemblages of G. duodenalis were identified by PCR assay of each gene in these fecal samples (Table 1). A 9.8% (10/102) of feline fecal samples were found to be positive by microscopy, 10% (3/30) in Baiyun district and 9.7% (7/72) in Conghua district. The tpi PCR was able to genotype 90.0% (9/10) of the Giardia-positive samples. Among them, 100% (9/9) of the samples were found to contain potentially zoonotic assemblage AI. The gdh PCR was able to genotype 40.0% (4/10) of the Giardia-positive samples. Among them, all the 4 samples were zoonotic assemblage AI. The bg PCR was able to genotype 60.0% (6/10) of the Giardia-positive samples. Among them, assemblage AI was the most prevalent, comprising of 66.7% (4/6) samples, followed by 1 assemblage C and 1 assemblage F. Only 30.0% (3/10) of the Giardia-positive samples were typed by the 18S rRNA PCR, sequence analysis revealed assemblage A in 2 isolates, and assemblage F in 1 isolate.


Occurrence and molecular identification of Giardia duodenalis from stray cats in Guangzhou, southern China.

Zheng G, Hu W, Liu Y, Luo Q, Tan L, Li G - Korean J. Parasitol. (2015)

PCR amplification of tpi (A), gdh (B), bg (C), and 18S rRNA (D) genes from the cat-derived Giardia. M, DL2000 DNA marker; 1,15-CBY19; 2,16-CBY20; 3,17-CHC79; 4-CHC83; 5,11,21- CHC66; 6-CHC77; 7,13-CHC62; 8-CHC80; 9,19,22-CHC68; 10-CHC83; 12,18-CHC77; 14,20-CBY01.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4384803&req=5

f1-kjp-53-1-119: PCR amplification of tpi (A), gdh (B), bg (C), and 18S rRNA (D) genes from the cat-derived Giardia. M, DL2000 DNA marker; 1,15-CBY19; 2,16-CBY20; 3,17-CHC79; 4-CHC83; 5,11,21- CHC66; 6-CHC77; 7,13-CHC62; 8-CHC80; 9,19,22-CHC68; 10-CHC83; 12,18-CHC77; 14,20-CBY01.
Mentions: The amplification fragments of the tpi (334 bp), gdh (530 bp), bg (515 bp), and 18S rRNA (291 bp) genes were obtained from 9, 4, 6, and 3 feline samples collected in Guangzhou, respectively (Fig. 1). The assemblages or sub-assemblages of G. duodenalis were identified by PCR assay of each gene in these fecal samples (Table 1). A 9.8% (10/102) of feline fecal samples were found to be positive by microscopy, 10% (3/30) in Baiyun district and 9.7% (7/72) in Conghua district. The tpi PCR was able to genotype 90.0% (9/10) of the Giardia-positive samples. Among them, 100% (9/9) of the samples were found to contain potentially zoonotic assemblage AI. The gdh PCR was able to genotype 40.0% (4/10) of the Giardia-positive samples. Among them, all the 4 samples were zoonotic assemblage AI. The bg PCR was able to genotype 60.0% (6/10) of the Giardia-positive samples. Among them, assemblage AI was the most prevalent, comprising of 66.7% (4/6) samples, followed by 1 assemblage C and 1 assemblage F. Only 30.0% (3/10) of the Giardia-positive samples were typed by the 18S rRNA PCR, sequence analysis revealed assemblage A in 2 isolates, and assemblage F in 1 isolate.

Bottom Line: Results show that 9.8% (10/102) feline fecal samples were found to be positive by microscopy, 10% (3/30) in Baiyun district and 9.7% (7/72) in Conghua district.Among the 10 positive samples, 9 were single infection (8 isolates, assemblage A; 1 isolate, assemblage F) and 1 sample was mixed infection with assemblages A and C.Based on tpi, gdh, and bg genes, all sequences of assemblage A showed complete homology with AI except for 1 isolate (CHC83).

View Article: PubMed Central - PubMed

Affiliation: College of Veterinary Medicine, South China Agricultural University, Guangzhou, 510642, People's Republic of China.

ABSTRACT
The objective of this study was to genetically characterize isolates of Giardia duodenalis and to determine if zoonotic potential of G. duodenalis could be found in stray cats from urban and suburban environments in Guangzhou, China. Among 102 fresh fecal samples of stray cats, 30 samples were collected in Baiyun district (urban) and 72 in Conghua district (suburban). G. duodenalis specimens were examined using light microscopy, then the positive specimens were subjected to PCR amplification and subsequent sequencing at 4 loci such as glutamate dehydrogenase (gdh), triose phosphate isomerase (tpi), β-giardin (bg), and small subunit ribosomal RNA (18S rRNA) genes. The phylogenetic trees were constructed using obtained sequences by MEGA5.2 software. Results show that 9.8% (10/102) feline fecal samples were found to be positive by microscopy, 10% (3/30) in Baiyun district and 9.7% (7/72) in Conghua district. Among the 10 positive samples, 9 were single infection (8 isolates, assemblage A; 1 isolate, assemblage F) and 1 sample was mixed infection with assemblages A and C. Based on tpi, gdh, and bg genes, all sequences of assemblage A showed complete homology with AI except for 1 isolate (CHC83). These findings not only confirmed the occurrence of G. duodenalis in stray cats, but also showed that zoonotic assemblage A was found for the first time in stray cats living in urban and suburban environments in China.

Show MeSH
Related in: MedlinePlus