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Subtype distribution of Blastocystis in Thai-Myanmar border, Thailand.

Popruk S, Udonsom R, Koompapong K, Mahittikorn A, Kusolsuk T, Ruangsittichai J, Palasuwan A - Korean J. Parasitol. (2015)

Bottom Line: The percentage of bootstrapped trees in which the associated taxa clustered together was relatively high.Some sequences of Blastocystis positive samples (TK18, 39, 46, 71, and 90) were closely related to animals (pig and cattle) indicating zoonotic risks.Therefore, proper health education in parasitic prevention for the villagers should be promoted to improve their personal hygiene.

View Article: PubMed Central - PubMed

Affiliation: Department of Protozoology, Faculty of Tropical Medicine, Mahidol University, 420/6 Ratchawithi Road, Bangkok, 10400, Thailand.

ABSTRACT
Blastocystis sp. is a common zoonotic intestinal protozoa which has been classified into 17 subtypes (STs). A cross-sectional study was conducted to determine the prevalence and subtype distribution of Blastocystis in villagers living on the Thai-Myanmar border, where the risk of parasitic infection is high. A total of 207 stool samples were collected and DNA was extracted. PCR and sequencing using primers targeting small-subunit ribosomal RNA (SSU rRNA) gene were performed. The prevalence of Blastocystis infection was 37.2% (77/207). ST3 (19.8%; 41/207) was the predominant subtype, followed by ST1 (11.6%; 24/207), ST2 (5.3%; 11/207), and ST4 (0.5%; 1/207). A phylogenetic tree was reconstructed using the maximum likelihood (ML) method based on the Hasegawa-Kishino-Yano + G + I model. The percentage of bootstrapped trees in which the associated taxa clustered together was relatively high. Some sequences of Blastocystis positive samples (TK18, 39, 46, 71, and 90) were closely related to animals (pig and cattle) indicating zoonotic risks. Therefore, proper health education in parasitic prevention for the villagers should be promoted to improve their personal hygiene. Further longitudinal studies are required to monitor the prevalence of parasitic infections after providing health education and to investigate Blastocystis ST in animals living in these villages.

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Maximum-likelihood phylogenetic analysis among SSU rDNA sequences of Blastocystis. Bold letters are sequences identified in this study
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f3-kjp-53-1-13: Maximum-likelihood phylogenetic analysis among SSU rDNA sequences of Blastocystis. Bold letters are sequences identified in this study

Mentions: The SSU rRNA genes were analyzed for polymorphisms of the DNA sequence, which indicated 139 parsimony informative sites, 46 singleton variable sites, and 185 variable sites from a total of 1,039 sites. Intraspecific divergence and interspecific divergence ranged from 0.01-2.1% and 3.5-10.8%, respectively. ML tree of Blastocystis inferred from SSU rRNA gene sequences was shown in Fig. 3. The ML method is based on the Hasegawa-Kishino-Yano+G+I model. The percentage of bootstrapped trees in which the associated taxa clustered together was relatively high. From the ML tree, the SSU rRNA gene sequences of Blastocystis positive samples compared with 17 reference sequences from available GenBank database showed that TK27, 58, 62, and 90 were classified in ST1, TK6 in ST2, TK18, 39, 46, 71, and 100 in ST3, and TK75 in ST4. Some sequences of Blastocystis positive samples (TK18, 39, 46, 71, and 90) were closely related to animals (pig and cattle).


Subtype distribution of Blastocystis in Thai-Myanmar border, Thailand.

Popruk S, Udonsom R, Koompapong K, Mahittikorn A, Kusolsuk T, Ruangsittichai J, Palasuwan A - Korean J. Parasitol. (2015)

Maximum-likelihood phylogenetic analysis among SSU rDNA sequences of Blastocystis. Bold letters are sequences identified in this study
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4384802&req=5

f3-kjp-53-1-13: Maximum-likelihood phylogenetic analysis among SSU rDNA sequences of Blastocystis. Bold letters are sequences identified in this study
Mentions: The SSU rRNA genes were analyzed for polymorphisms of the DNA sequence, which indicated 139 parsimony informative sites, 46 singleton variable sites, and 185 variable sites from a total of 1,039 sites. Intraspecific divergence and interspecific divergence ranged from 0.01-2.1% and 3.5-10.8%, respectively. ML tree of Blastocystis inferred from SSU rRNA gene sequences was shown in Fig. 3. The ML method is based on the Hasegawa-Kishino-Yano+G+I model. The percentage of bootstrapped trees in which the associated taxa clustered together was relatively high. From the ML tree, the SSU rRNA gene sequences of Blastocystis positive samples compared with 17 reference sequences from available GenBank database showed that TK27, 58, 62, and 90 were classified in ST1, TK6 in ST2, TK18, 39, 46, 71, and 100 in ST3, and TK75 in ST4. Some sequences of Blastocystis positive samples (TK18, 39, 46, 71, and 90) were closely related to animals (pig and cattle).

Bottom Line: The percentage of bootstrapped trees in which the associated taxa clustered together was relatively high.Some sequences of Blastocystis positive samples (TK18, 39, 46, 71, and 90) were closely related to animals (pig and cattle) indicating zoonotic risks.Therefore, proper health education in parasitic prevention for the villagers should be promoted to improve their personal hygiene.

View Article: PubMed Central - PubMed

Affiliation: Department of Protozoology, Faculty of Tropical Medicine, Mahidol University, 420/6 Ratchawithi Road, Bangkok, 10400, Thailand.

ABSTRACT
Blastocystis sp. is a common zoonotic intestinal protozoa which has been classified into 17 subtypes (STs). A cross-sectional study was conducted to determine the prevalence and subtype distribution of Blastocystis in villagers living on the Thai-Myanmar border, where the risk of parasitic infection is high. A total of 207 stool samples were collected and DNA was extracted. PCR and sequencing using primers targeting small-subunit ribosomal RNA (SSU rRNA) gene were performed. The prevalence of Blastocystis infection was 37.2% (77/207). ST3 (19.8%; 41/207) was the predominant subtype, followed by ST1 (11.6%; 24/207), ST2 (5.3%; 11/207), and ST4 (0.5%; 1/207). A phylogenetic tree was reconstructed using the maximum likelihood (ML) method based on the Hasegawa-Kishino-Yano + G + I model. The percentage of bootstrapped trees in which the associated taxa clustered together was relatively high. Some sequences of Blastocystis positive samples (TK18, 39, 46, 71, and 90) were closely related to animals (pig and cattle) indicating zoonotic risks. Therefore, proper health education in parasitic prevention for the villagers should be promoted to improve their personal hygiene. Further longitudinal studies are required to monitor the prevalence of parasitic infections after providing health education and to investigate Blastocystis ST in animals living in these villages.

Show MeSH
Related in: MedlinePlus