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Subtype distribution of Blastocystis in Thai-Myanmar border, Thailand.

Popruk S, Udonsom R, Koompapong K, Mahittikorn A, Kusolsuk T, Ruangsittichai J, Palasuwan A - Korean J. Parasitol. (2015)

Bottom Line: The percentage of bootstrapped trees in which the associated taxa clustered together was relatively high.Some sequences of Blastocystis positive samples (TK18, 39, 46, 71, and 90) were closely related to animals (pig and cattle) indicating zoonotic risks.Therefore, proper health education in parasitic prevention for the villagers should be promoted to improve their personal hygiene.

View Article: PubMed Central - PubMed

Affiliation: Department of Protozoology, Faculty of Tropical Medicine, Mahidol University, 420/6 Ratchawithi Road, Bangkok, 10400, Thailand.

ABSTRACT
Blastocystis sp. is a common zoonotic intestinal protozoa which has been classified into 17 subtypes (STs). A cross-sectional study was conducted to determine the prevalence and subtype distribution of Blastocystis in villagers living on the Thai-Myanmar border, where the risk of parasitic infection is high. A total of 207 stool samples were collected and DNA was extracted. PCR and sequencing using primers targeting small-subunit ribosomal RNA (SSU rRNA) gene were performed. The prevalence of Blastocystis infection was 37.2% (77/207). ST3 (19.8%; 41/207) was the predominant subtype, followed by ST1 (11.6%; 24/207), ST2 (5.3%; 11/207), and ST4 (0.5%; 1/207). A phylogenetic tree was reconstructed using the maximum likelihood (ML) method based on the Hasegawa-Kishino-Yano + G + I model. The percentage of bootstrapped trees in which the associated taxa clustered together was relatively high. Some sequences of Blastocystis positive samples (TK18, 39, 46, 71, and 90) were closely related to animals (pig and cattle) indicating zoonotic risks. Therefore, proper health education in parasitic prevention for the villagers should be promoted to improve their personal hygiene. Further longitudinal studies are required to monitor the prevalence of parasitic infections after providing health education and to investigate Blastocystis ST in animals living in these villages.

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Gel electrophoresis of PCR amplification. Lane 1, 100 bp DNA marker; Lane 2, negative control; Lane 3, positive control with Blastocystis; and Lanes 4-6, positive samples with Blastocystis.
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f2-kjp-53-1-13: Gel electrophoresis of PCR amplification. Lane 1, 100 bp DNA marker; Lane 2, negative control; Lane 3, positive control with Blastocystis; and Lanes 4-6, positive samples with Blastocystis.

Mentions: The prevalence of Blastocystis infection in this study was 37.2 % (77/207) by nested PCR method. Fig. 2 shows the positive PCR results of Blastocystis. The PCR products were shown to be about 1,100 base pairs (bp) long.


Subtype distribution of Blastocystis in Thai-Myanmar border, Thailand.

Popruk S, Udonsom R, Koompapong K, Mahittikorn A, Kusolsuk T, Ruangsittichai J, Palasuwan A - Korean J. Parasitol. (2015)

Gel electrophoresis of PCR amplification. Lane 1, 100 bp DNA marker; Lane 2, negative control; Lane 3, positive control with Blastocystis; and Lanes 4-6, positive samples with Blastocystis.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4384802&req=5

f2-kjp-53-1-13: Gel electrophoresis of PCR amplification. Lane 1, 100 bp DNA marker; Lane 2, negative control; Lane 3, positive control with Blastocystis; and Lanes 4-6, positive samples with Blastocystis.
Mentions: The prevalence of Blastocystis infection in this study was 37.2 % (77/207) by nested PCR method. Fig. 2 shows the positive PCR results of Blastocystis. The PCR products were shown to be about 1,100 base pairs (bp) long.

Bottom Line: The percentage of bootstrapped trees in which the associated taxa clustered together was relatively high.Some sequences of Blastocystis positive samples (TK18, 39, 46, 71, and 90) were closely related to animals (pig and cattle) indicating zoonotic risks.Therefore, proper health education in parasitic prevention for the villagers should be promoted to improve their personal hygiene.

View Article: PubMed Central - PubMed

Affiliation: Department of Protozoology, Faculty of Tropical Medicine, Mahidol University, 420/6 Ratchawithi Road, Bangkok, 10400, Thailand.

ABSTRACT
Blastocystis sp. is a common zoonotic intestinal protozoa which has been classified into 17 subtypes (STs). A cross-sectional study was conducted to determine the prevalence and subtype distribution of Blastocystis in villagers living on the Thai-Myanmar border, where the risk of parasitic infection is high. A total of 207 stool samples were collected and DNA was extracted. PCR and sequencing using primers targeting small-subunit ribosomal RNA (SSU rRNA) gene were performed. The prevalence of Blastocystis infection was 37.2% (77/207). ST3 (19.8%; 41/207) was the predominant subtype, followed by ST1 (11.6%; 24/207), ST2 (5.3%; 11/207), and ST4 (0.5%; 1/207). A phylogenetic tree was reconstructed using the maximum likelihood (ML) method based on the Hasegawa-Kishino-Yano + G + I model. The percentage of bootstrapped trees in which the associated taxa clustered together was relatively high. Some sequences of Blastocystis positive samples (TK18, 39, 46, 71, and 90) were closely related to animals (pig and cattle) indicating zoonotic risks. Therefore, proper health education in parasitic prevention for the villagers should be promoted to improve their personal hygiene. Further longitudinal studies are required to monitor the prevalence of parasitic infections after providing health education and to investigate Blastocystis ST in animals living in these villages.

Show MeSH
Related in: MedlinePlus