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Diphyllobothrium nihonkaiense infections in a family.

Go YB, Lee EH, Cho J, Choi S, Chai JY - Korean J. Parasitol. (2015)

Bottom Line: They were treated with a single oral dose of praziquantel and then complained of no more symptoms.This is the first report of D. nihonkaiense infection in a family in Korea, and this report includes the 8th pediatric case in Korea.The current report is meaningful because D. nihonkaiense infection within a family is rare.

View Article: PubMed Central - PubMed

Affiliation: Department of Pediatrics, Myongji Hospital, Goyang 412-826, Korea.

ABSTRACT
Diphyllobothrium latum and Diphyllobothrium nihonkaiense are morphologically similar to each other, and only genetic method can differentiate clearly between the 2 species. A strobila of diphyllobothriid tapeworm discharged from a 7-year-old boy was analyzed to identify the species by mitochondrial cytochrome c oxidase subunit 1 (cox1) gene sequencing. He and his family (total 4 persons) ate slices of 3 kinds of raw fish 16 days before visiting our outpatient clinic. All family members complained of abdominal pain and watery diarrhea. They all expelled tapeworm strobilae in their stools. They were treated with a single oral dose of praziquantel and then complained of no more symptoms. The cox1 gene sequencing of the strobila from the boy revealed 99.9% (687/688 bp) similarity with D. nihonkaiense and only 93.2% (641/688 bp) similarity with D. latum. Thus, we assigned this tapeworm as D. nihonkaiense. This is the first report of D. nihonkaiense infection in a family in Korea, and this report includes the 8th pediatric case in Korea. The current report is meaningful because D. nihonkaiense infection within a family is rare.

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Related in: MedlinePlus

Neighbor-joining tree of human Diphyllobothrium tapeworms based on nucleotide sequences of the cox1 gene. Numbers above the branches detect the bootstrap values (1,000 replicates). The scale bar represents the estimated number of nucleotide substitutions per nucleotide site. The phylogenetic tree reveals that the cox1 gene from our case was closer to D. nihonkaiense than D. latum.
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f1-kjp-53-1-109: Neighbor-joining tree of human Diphyllobothrium tapeworms based on nucleotide sequences of the cox1 gene. Numbers above the branches detect the bootstrap values (1,000 replicates). The scale bar represents the estimated number of nucleotide substitutions per nucleotide site. The phylogenetic tree reveals that the cox1 gene from our case was closer to D. nihonkaiense than D. latum.

Mentions: The tapeworm specimen was fixed in 10% formalin, and sent to the Department of Parasitology and Tropical Medicine, Seoul National University College of Medicine, Seoul, Korea. Formalin was substituted with 1× PBS to remove the formalin for 24 hr. Before the molecular identification, the sample was identified as Diphyllobothrium sp., according to the microscopic examinations (×400 magnification) of eggs extracted from the uteri. Genomic DNA was then extracted from a single proglottid following a spin-column protocol using DNeasy Blood & Tissue Kit (Ǫiagen, Hilden, Germany). The partial cox1 gene was amplified by PCR using the forward primer (D1/nco1f1 5’-TAG CTG CTG CTA TAC AAT GTT GTT ATT-3’) and the reverse primer (D1/nco1r1 5’-ACG ACG TGG TAA ACG GCA CAC ACC AAA-3’). PCR was carried out with 1 cycle of 94˚C for 5 min, 35 cycles of 94˚C for 1 min, 46˚C for 1 min, and 72˚C for 1 min, plus a final cycle at 72˚C for 5 min. DNA sequencing and construction of a neighbor-joining tree using our samples were performed according to the protocol described by Jeon et al. [8]. The reference data we used for a phylogenetic study were D. nihonkaiense (GenBank no. AB684622, EF420138, AB268585), D. latum (AB504899, DQ985706), and S. erinaceieuropaei (AB369250). The cox1 sequences (688 bp) of the tapeworm showed 99.9% (687/688 bp) similarity with the reference sequence of D. nihonkaiense (EF420138), whereas the similarity with D. latum (DQ985706) was 93.2% (641/688 bp). Thus, the pathogen was identified as D. nihonkaiense (Fig. 1).


Diphyllobothrium nihonkaiense infections in a family.

Go YB, Lee EH, Cho J, Choi S, Chai JY - Korean J. Parasitol. (2015)

Neighbor-joining tree of human Diphyllobothrium tapeworms based on nucleotide sequences of the cox1 gene. Numbers above the branches detect the bootstrap values (1,000 replicates). The scale bar represents the estimated number of nucleotide substitutions per nucleotide site. The phylogenetic tree reveals that the cox1 gene from our case was closer to D. nihonkaiense than D. latum.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4384796&req=5

f1-kjp-53-1-109: Neighbor-joining tree of human Diphyllobothrium tapeworms based on nucleotide sequences of the cox1 gene. Numbers above the branches detect the bootstrap values (1,000 replicates). The scale bar represents the estimated number of nucleotide substitutions per nucleotide site. The phylogenetic tree reveals that the cox1 gene from our case was closer to D. nihonkaiense than D. latum.
Mentions: The tapeworm specimen was fixed in 10% formalin, and sent to the Department of Parasitology and Tropical Medicine, Seoul National University College of Medicine, Seoul, Korea. Formalin was substituted with 1× PBS to remove the formalin for 24 hr. Before the molecular identification, the sample was identified as Diphyllobothrium sp., according to the microscopic examinations (×400 magnification) of eggs extracted from the uteri. Genomic DNA was then extracted from a single proglottid following a spin-column protocol using DNeasy Blood & Tissue Kit (Ǫiagen, Hilden, Germany). The partial cox1 gene was amplified by PCR using the forward primer (D1/nco1f1 5’-TAG CTG CTG CTA TAC AAT GTT GTT ATT-3’) and the reverse primer (D1/nco1r1 5’-ACG ACG TGG TAA ACG GCA CAC ACC AAA-3’). PCR was carried out with 1 cycle of 94˚C for 5 min, 35 cycles of 94˚C for 1 min, 46˚C for 1 min, and 72˚C for 1 min, plus a final cycle at 72˚C for 5 min. DNA sequencing and construction of a neighbor-joining tree using our samples were performed according to the protocol described by Jeon et al. [8]. The reference data we used for a phylogenetic study were D. nihonkaiense (GenBank no. AB684622, EF420138, AB268585), D. latum (AB504899, DQ985706), and S. erinaceieuropaei (AB369250). The cox1 sequences (688 bp) of the tapeworm showed 99.9% (687/688 bp) similarity with the reference sequence of D. nihonkaiense (EF420138), whereas the similarity with D. latum (DQ985706) was 93.2% (641/688 bp). Thus, the pathogen was identified as D. nihonkaiense (Fig. 1).

Bottom Line: They were treated with a single oral dose of praziquantel and then complained of no more symptoms.This is the first report of D. nihonkaiense infection in a family in Korea, and this report includes the 8th pediatric case in Korea.The current report is meaningful because D. nihonkaiense infection within a family is rare.

View Article: PubMed Central - PubMed

Affiliation: Department of Pediatrics, Myongji Hospital, Goyang 412-826, Korea.

ABSTRACT
Diphyllobothrium latum and Diphyllobothrium nihonkaiense are morphologically similar to each other, and only genetic method can differentiate clearly between the 2 species. A strobila of diphyllobothriid tapeworm discharged from a 7-year-old boy was analyzed to identify the species by mitochondrial cytochrome c oxidase subunit 1 (cox1) gene sequencing. He and his family (total 4 persons) ate slices of 3 kinds of raw fish 16 days before visiting our outpatient clinic. All family members complained of abdominal pain and watery diarrhea. They all expelled tapeworm strobilae in their stools. They were treated with a single oral dose of praziquantel and then complained of no more symptoms. The cox1 gene sequencing of the strobila from the boy revealed 99.9% (687/688 bp) similarity with D. nihonkaiense and only 93.2% (641/688 bp) similarity with D. latum. Thus, we assigned this tapeworm as D. nihonkaiense. This is the first report of D. nihonkaiense infection in a family in Korea, and this report includes the 8th pediatric case in Korea. The current report is meaningful because D. nihonkaiense infection within a family is rare.

Show MeSH
Related in: MedlinePlus